, Volume 38, Issue 8, pp 805–811 | Cite as

Supplementation with CLA: Isomer incorporation into serum lipids and effect on body fat of women

  • Anatoli Petridou
  • Vassilis Mougios
  • Angelos Sagredos


Animal studies have suggested that CLA, a natural component of meat and dairy products, may confer beneficial effects on health. However, human studies using supplementation with CLA have produced contradictory results. The aim of the present study was to further investigate the effect of CLA supplementation on human body fat, serum leptin, and serum lipids, as well as the incorporation of CLA isomers into serum lipids classes. Sixteen young healthy nonobese sedentary women received 2.1 g of CLA (divided equally between the cis,trans-9,11 and trans,cis-10,12 isomers) daily for 45 d and placebo for 45 d in a randomized double-blind crossover design. Body fat was estimated (by measurement of skinfold thickness at 10 sites), and blood was sampled at the beginning, middle, and end of the entire intervention period; an additional blood sample was obtained 2 wk thereafer. No significant differences in energy, carbohydrate, lipid, or protein intake existed between the CLA and placebo intake periods. No significant differences were found in body fat or serum leptin, TAG, total cholesterol, HDL-cholesterol, and alanine aminotransferase between CLA and placebo. The CLA isomer content of serum TAG, phospholipids, and total lipids increased 2–5 times with CLA supplementation (P<0.05). In contrast, the CLA content of cholesteryl esters did not change significantly. The period of 2 wk after the end of CLA supplementation was sufficient for its washout from serum lipids. These data indicate that supplementation with 2.1 g of CLA daily for 45 d increased its levels in blood but had no effect on body composition or the lipidemic profile of nonobese women.



alanine aminotransferase


cholesteryl esters




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  1. 1.
    Pariza, M.W., Park, Y., and Cook, M.E. (2001) The Biologically Active Isomers of Conjugated Linoleic Acid, Progr. Lipid Res. 40, 283–298.CrossRefGoogle Scholar
  2. 2.
    Blankson, H., Stakkestad, J.A., Fagertun, H., Thom, E., Wadstein, J., and Gudmundsen, O. (2000) Conjugated Linoleic Acid Reduces Body Fat Mass in Overweight and Obese Humans, J. Nutr. 130, 2943–2948.PubMedGoogle Scholar
  3. 3.
    Mougios, V., Matsakas, A., Petridou, A., Ring, S., Sagredos, A., Melissopoulou, A., Tsigilis, N., and Nikolaidis, M. (2001) Effect of Supplementation with Conjugated Linoleic Acid on Human Serum Lipids and Body Fat, J. Nutr. Biochem. 12, 585–592.PubMedCrossRefGoogle Scholar
  4. 4.
    Riserus, U., Berglund, L., and Vessby, B. (2001) Conjugated Linoleic Acid (CLA) Reduced Abdominal Adipose Tissue in Obese Middle-Aged Men with Signs of the Metabolic Syndrome: A Randomised Controlled Trial, Int. J. Obes. Relat. Metab. Disord. 25, 1129–1135.PubMedCrossRefGoogle Scholar
  5. 5.
    Smedman, A., and Vessby, B. (2001) Conjugated Linoleic Acid Supplementation in Humans—Metabolic Effects, Lipids 36, 773–781.PubMedCrossRefGoogle Scholar
  6. 6.
    Thom, E., Wadstein, J., and Gudmundsen, O. (2001) Conjugated Linoleic Acid Reduces Body Fat in Healthy Exercising Humans, J. Int. Med. Res. 29, 392–396.PubMedGoogle Scholar
  7. 7.
    Atkinson, R.L. (1999) Conjugated Linoleic Acid for Altering Body Composition and Treating Obesity, in Advances in Conjugated Linoleic Acid Research, Vol. 1 (Yurawecz, M.P., Mossoba, M.M., Kramer, J.K.G., Pariza, M.W., and Nelson, G., eds.), pp. 348–353, AOCS Press, Champaign.Google Scholar
  8. 8.
    Zambell, K.L., Keim, N.L., Van Loan, M.D., Gale, B., Benito, P., Kelley, D.S., and Nelson, G.J. (2000) Conjugated Linoleic Acid Supplementation in Humans: Effects on Body Composition and Energy Expenditure, Lipids 35, 777–782.PubMedGoogle Scholar
  9. 9.
    Kreider, R.B., Wilson, M., Ferreira, M.P., Greenwood, M., and Almada, L.A. (2002) Effects of Conjugated Linoleic Acid Supplementation During Resistance Training on Body Composition, Bone Density, Strength, and Selected Hematological Markers, J. Strength Cond. Res. 16, 325–334.PubMedCrossRefGoogle Scholar
  10. 10.
    Benito, P., Nelson, G.J., Kelley, D.S., Bartolini, G., Schmidt, P.C., and Simon, V. (2001) The Effect of Conjugated Linoleic Acid on Plasma Lipoproteins and Tissue Fatty Acid Composition in Humans, Lipids 36, 229–236.PubMedCrossRefGoogle Scholar
  11. 11.
    Parizkova, J. (1968) Body Composition and Physical Fitness, Curr. Anthropol. 9, 273–287.CrossRefGoogle Scholar
  12. 12.
    Williams, C.A., and Bale, P. (1998) Bias and Llimits of Agreement Between Hydrodensitometry, Bioelectrical Impedance and Skinfold Calipers Measures of Percentage Body Fat, Eur. J. Appl. Physiol. Occup. Physiol. 77, 271–277.PubMedCrossRefGoogle Scholar
  13. 13.
    Kramer, J.K.G., Fellner, V., Dugan, M.E.R., Sauer, F.D., Mossoba, M.D., and Yurawecz, M.P. (1997) Evaluating Acid and Base Catalysts in the Methylation of Milk and Rumen Fatty Acids with Special Emphasis on Conjugated Dienes and Total trans Fatty Acids, Lipids 32, 1219–1228.PubMedCrossRefGoogle Scholar
  14. 14.
    Holland, B., Welch, A.A., Unwin, I.D., Buss, D.H., Paul, A.A., and Southgate, D.A.T. (1991) McCance and Widdowson's the Composition of Foods, Royal Society of Chemistry, Cambridge, United Kingdom.Google Scholar
  15. 15.
    Fellingham, G.W., Bryce, G.R., and Carter, M.W. (1978) Latin Square Changeover Design in Physical Education Research, Res. Quart. 49, 125–134.Google Scholar
  16. 16.
    Park, S.J., Park, C.W., Kim, S.J., Kim, J.K., Kim, Y.R., Park, K.A., Kim, J.O., and Ha, Y.L. (2002) Methylation Methods for the Quantitative Analysis of Conjugated Linoleic Acid (CLA) Isomers in Various Lipid Samples, J. Agric. Food Chem. 50, 989–996.PubMedCrossRefGoogle Scholar
  17. 17.
    Werner, S.A., Luedecke, L.O., and Shultz, T.D. (1992) Determination of Conjugated Linoleic Acid Content and Isomer Distribution in Three Cheddar-type Cheeses: Effects of Cheese Cultures, Processing, and Aging, J. Agric. Food Chem. 40, 1817–1821.CrossRefGoogle Scholar
  18. 18.
    Lönnqvist, F., Nordfors, L., Jansson, M., Thörne, A., Schalling, M., and Arner, P. (1997) Leptin Secretion from Adipose Tissue in Women. Relationship to Plasma Levels and Gene Expression, J. Clin. Invest. 99, 2398–2404.PubMedCrossRefGoogle Scholar
  19. 19.
    Medina, E.A., Horn, W.F., Keim, N.L., Havel, P.J., Benito, P., Kelley, D.S., Nelson, G.J., and Erickson, K.L. (2000) Conjugated Linoleic Acid Supplementation on Humans: Effects on Circulating Leptin Concentrations and Appetite, Lipids 35, 783–788.PubMedGoogle Scholar
  20. 20.
    Noone, E.J., Roche, H.M., Nugent, A.P., and Gibney, M.J. (2002) The Effects of Dietary Supplementation Using Isomeric Blends of Conjugated Linoleic Acid on Lipid Metabolism in Healthy Human Subjects, Br. J. Nutr. 88, 243–251.PubMedCrossRefGoogle Scholar
  21. 21.
    Khosla, P., and Fungwe, T.V. (2001) Conjugated Linoleic Acid: Effects on Plasma Lipids and Cardiovascular Function, Curr. Opin. Lipidol. 12, 31–34.PubMedCrossRefGoogle Scholar
  22. 22.
    Belury, M.A., Moya-Camarena, S.Y., Liu, K.L., and Vanden-Heuvel, J.P. (1997) Dietary Conjugated Linoleic Acid Induces Peroxisome-Specific Enzyme Accumulation and Ornithine Decarboxylase Activity in Mouse Liver, J. Nutr. Biochem. 8, 579–584.CrossRefGoogle Scholar
  23. 23.
    Fogerty, A.C., Ford, G.L., and Svoronos, D. (1988) Octadeca-9,11-dienoic Acid in Foodstuffs and in the Lipids of Human Blood and Breast Milk, Nutr. Rep. Int. 38, 937–944.Google Scholar
  24. 24.
    Ha, Y.L., Grimm, N.K., and Pariza, M.W. (1989) Newly Recognized Anticarcinogenic Fatty Acids: Identification and Quantification in Natural and Processed Cheeses, J. Agric. Food Chem. 37, 75–81.CrossRefGoogle Scholar

Copyright information

© AOCS Press 2003

Authors and Affiliations

  • Anatoli Petridou
    • 1
  • Vassilis Mougios
    • 1
  • Angelos Sagredos
    • 2
  1. 1.Department of Physical Education and Sport ScienceTEFAA, University of ThessalonikiThessalonikiGreece
  2. 2.Department of Physical Education and Sport ScienceConsultingHamburgGermany

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