Antagonism of croton oil inflammation by topical emu oil in CD-1 mice
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Emu oil is derived from the emu (Dromaius novaehollandiae), which originated in Australia, and has been reported to have anti-inflammatory properties. Inflammation was induced in anesthetized CD-1 mice by applying 50 μL of 2% croton oil to the inner surface of the left ear. After 2 h, the area was treated with 5μL of emu, fish, flaxseed, olive, or liquified chicken fat, or left untreated. Animals were euthanized at 6 h postapplication of different oils, and earplugs (FP) and plasma samples were collected. Inflammation was evaluated by change in earlobe thickness, increase in weight of EP tissue (compared to the untreated ear), and induction in cytokines interleukin (IL)-1α and tumor necrosis factor-α (TNF-α) in EP homogenates. Al-though reductions relative to control (croton oil) were noted for all treatments, auricular thickness and EP weights were, significantly reduced (−72 and −71%, respectively) only in the emu oil-treated group. IL-1α levels in homogenates of auricular tissue were significantly reduced in the fish oil (−57%) and emu oil (−70%) groups relative to the control group. The cytokine TNF-α from auricular homogenates was significantly reduced in the olive oil (−52%) and emu oil (−60%) treatment groups relative to the control group. Plasma cytokine levels were not changed by croton oil treatment. Although auricular thickness and weight were significantly correlated with each other (r=0.750, P<0.003), auricular thickness but not weight was significantly correlated with cytokine IL-1α (r=0.750, P<0.006) and TNF-α (r=0.690, P<0.02). These studies indicate that topical emu oil has anti-inflammatory properties in the CD-1 mouse that are associated with decreased auricular thickness and weight, and with the cytokines IL-1α and TNF-α.
KeywordsCroton Hesperidin Polysporin Common Superscript Roman Letter Periapical Bone Destruction
tumor necrosis factor-α
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- 3.Whitehouse, M.W., and Turner, A.G. (1997) Emu Oil(s): A Source of Non-toxic Transdermal Anti-inflammatory Agents in Aborginal Medicine, Am. Emu Assoc. Newslett. 6, 15–16.Google Scholar
- 14.Huang, M.T., Ho, C.T., Wang, Z.Y., Ferraro, T., Finnegan-Olive, T., Lou, Y.R., Mitchell, J.M., Laskin, J.D., Newmark, H., Yang, C.S., and Conney, A.H. (1992) Inhibitory Effect of Topical Application of a Green Tea Polyphenol Fraction on Tumour Initiation and Promotion in Mouse Skin, Carcinogenesis 13, 947–954.PubMedGoogle Scholar
- 16.Hamsen, M., Lerche, A., Kassis, V., Lorenzen., J., and Sondergaard, J. (1983) Treatment of Rheumatoid Arthritis with Prostaglandin E1, Precursors, cis-Linolenic Acid and γ-Linolenic Acid, Scand. J. Rheumatol 12, 85–88.Google Scholar
- 20.Berger, A., Monnard, I., Baur, M., Charbonnet, C., Safonova, I., and Jomard, A. (2002) Epidermal Anti-inflammatory Properties of 5,11,14, 20∶3: Effects on Mouse Ear Edema, PGE2 Levels in Cultured Keratinocytes and PPAR Activation, Lipids Health Dis. 1, 12–17.Google Scholar
- 25.Instute of Laboratory Animal Research, 1996, Guide for the Care and Use of Laboratory Animals, 140 pp. National Academies Press, Washington, D.C.Google Scholar
- 26.Matsumoto, K., Fujimoto, M., Ito, K., Tanaka, H., and Hirano, I. (1990) Comparison of the Effects of Biolobol and 12-O-Tetradecanoylphenol-13-acetate on Skin, and Test of Tumor Promoting Potential of Bilobol in CD-1 Mice, Toxicol. Sci. 15, 39–46.Google Scholar
- 27.Wixson, S.K. (1994) Rabbits and Rodents: Anesthesia and Analgesia, in Research Animal Anesthesia, Analgesia and Surgery (Smith, A.C., and Swindle, M.M., eds.), Scientist Center for Animal Welfare, Greebelt, Maryland, pp. 59–71.Google Scholar
- 30.Snedecor, G.W., and Cochran, W.G. (1980) Statistical Methods, The Iowa State University Press, Ames, Iowa.Google Scholar