Lipids

, Volume 37, Issue 9, pp 925–928 | Cite as

Apolipoproteins A-I and A-II downregulate neutrophil functions

  • Cristiane Jaciara Furlaneto
  • Fernanda Pereira Ribeiro
  • Elaine Hatanaka
  • Glaucia Mendes Souza
  • Marco A. Cassatella
  • Ana Campa
Communication

Abstract

This work reports the effect of the apolipoproteins A-I and A-II (apoA-I and apoA-II) on the release of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-8, and IL-1 receptor antagonist (IL-1Ra) and on the oxidative burst of human neutrophils. By themselves, apoA-I and apoA-II do not affect the basal liberation of these cytokines, whereas apoA-I affects the release of IL-1β from lipopolysaccharide (LPS)-stimulated neutrophils and apoA-II affects IL-8 released from LPS-stimulated neutrophils. ApoA-II also decreases the production of IL-8 released by neutrophils stimulated with the acute phase apolipoprotein serum amyloid A. Both apoA-I and apoA-II exerted ∼30% inhibition on the oxidative burst of neutrophils stimulated by opsonized zymosan, as revealed by the luminol-enhanced chemiluminescence assay. These findings give additional support to the idea that the role of human plasma lipoproteins and apolipoproteins goes beyond their function in lipid transport and metabolism. HDL apolipoproteins appear to be a class of mediators that can participate in the regulation of the activity of neutrophils.

Abbreviations

apoA-I

apolipoproteins A-I

apoA-II

apolipoproteins A-II

IL

interleukin

IL-1Ra

interleukin-1 receptor antagonist

LPS

hypopolysaccharide

RPA

RNAse protection assay

SAA

serum amyloid A

TNF

tumor necrosis factor

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Copyright information

© AOCS Press 2002

Authors and Affiliations

  • Cristiane Jaciara Furlaneto
    • 1
  • Fernanda Pereira Ribeiro
    • 1
  • Elaine Hatanaka
    • 1
  • Glaucia Mendes Souza
    • 2
  • Marco A. Cassatella
    • 3
  • Ana Campa
    • 1
  1. 1.Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências FarmacêuticasUniversidade de São PauloSão PauloBrazil
  2. 2.Departamento de Bioquímica, Instituto de QuímicaUniversidade de São PauloSão PauloBrazil
  3. 3.Departamento di Patologia, Sezione di Patologia GeneraleUniversitá di VeronaVeronaItaly

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