Lipids

, Volume 37, Issue 1, pp 27–32

Assessment of dietary and genetic factors influencing serum and adipose fatty acid composition in obese female identical twins

  • Marie Kunešová
  • Vojtěch Hainer
  • Eva Tvrzická
  • Stephen D. Phinney
  • Vladimír Štich
  • Jana Pařízková
  • Aleš Zák
  • Albert J. Stunkard
Articles

DOI: 10.1007/s11745-002-0860-z

Cite this article as:
Kunešová, M., Hainer, V., Tvrzická, E. et al. Lipids (2002) 37: 27. doi:10.1007/s11745-002-0860-z

Abstract

Fourteen pairs of obese female monozygotic twins were recruited for a study of genetic influences on serum and adipose fatty acid (FA) composition. Following 1 wk of inpatient stabilization, fasting serum and adipose tissue obtained by surgical excision were analyzed by thin-layer and gas chromatography. Intrapair resemblances (IPR) for individual FA were assessed by Spearman rank correlation and by analysis of variance and were found in serum cholesteryl esters (CE), triglycerides (TG), and adipose TG. With two exceptions (CE linoleate and adipose eicosapentaenoate), these IPR were limited to the nonessential FA. Palmitate had significant IPR in four lipid fractions; in serum CE and adipose TG palmitate was strongly correlated with multiple measures of adiposity. In contrast to other lipid fractions, serum phosphatidylcholine (PC) FA had 12 IPR, of which 6 were essential FA including arachidonate (r=0.76, P<0.0005), eicosapentaenoate (r=0.78, P<0.0005), and docosahexaenoate (r=0.86, P<0.0001). The PC IPR could not be explained by analysis of preadmission 7-d food records. After dividing the pairs into two groups differing and nondiffering according to fat intake of individuals in the pair, there was no evidence of a gene-environment interaction between fat intake and FA composition. The IPR for nonessential FA indicate that there is active genetic control of either food choices or postabsorptive metabolic processing. The high level of IPR in the PC fraction in contrast to the other lipid fractions suggests strong genetic influence over selection of specific FA for this membrane fraction independent of diet.

Abbreviations

AT

adipose tissue

CE

cholesteryl esters

FA

fatty acid(s)

FAME

fatty acid methyl esters

GC

gas chromatography

IPR

intra-pair resemblance

PC

phosphatidylcholine

TG

triglycerides

Copyright information

© AOCS Press 2002

Authors and Affiliations

  • Marie Kunešová
    • 5
  • Vojtěch Hainer
    • 5
  • Eva Tvrzická
    • 1
  • Stephen D. Phinney
    • 2
  • Vladimír Štich
    • 3
  • Jana Pařízková
    • 5
  • Aleš Zák
    • 1
  • Albert J. Stunkard
    • 4
  1. 1.Fourth Medical DepartmentCharles UniversityPrague
  2. 2.Galileo LaboratoriesSanta Clara
  3. 3.Third Medical SchoolCharles UniversityPragueCzech Republic
  4. 4.Department of PsychiatryUniversity of PennsylvaniaPhiladelphia
  5. 5.Obesity Management Centre, Third Medical Department, First Medical SchoolCharles UniversityPragueCzech Republic

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