Lipids

, 36:461 | Cite as

Effects of dietary defatted squid on cholesterol metabolism and hepatic lipogenesis in rats

  • Kazunari Tanaka
  • Ikuo Ikeda
  • Hiroko Yoshida
  • Katsumi Imaizumi
Articles

Abstract

Male Sprague-Dawley rats were fed a cholesterol-free (Exp. 1) or cholesterol-supplemented (Exp. 2) diet containing 20% casein (control group) or 15% defatted squid and 5% casein (defatted squid group), as protein, for 14 d. Serum and hepatic cholesterol concentrations were lower in rats fed defatted squid than in those fed casein in both cholesterol-free (−20%, P<0.05 and −15%, P<0.05, respectively) and cholesterol-supplemented (−25%, P<0.05 and −15%, P<0.05, respectively) diets. Hepatic triglyceride concentration was lower in the defatted squid than in the control groups in both cholesterol-free (−51%, P<0.05) and cholesterol-supplemented diets (−38%, P<0.01). The activities of cytosolic fatty acid synthase and the NADPH-generating enzymes, malic enzyme and glucose-6-phosphate dehydrogenase, in the liver were lower in the defatted squid than in the control groups in both cholesterol-free (−21%, P<0.01, −33%, P<0.05, and −33%, P<0.01, respectively) and cholesterol-supplemented diets (−34%, P<0.05, −57%, P<0.05, and −67%, P<0.05, respectively). The activity of mitochondrial carnitine palmitoyltransferase in the liver was comparable between the control and defatted squid groups. The activity of Mg2+-dependent phosphatidate phosphohydrolase in the liver cytosol was lower in the defatted squid (−9%, P<0.05) than in the control groups only in the cholesterol-free diet. Fecal excretion of total steroids was stimulated by the feeding of defatted squid in both cholesterol-free (+77%, P<0.005) and cholesterol-supplemented diets (+29%, P<0.01). These results suggest that the nonlipid fraction of squid exerts a hypocholesterolemic effect by increasing the excretion of total steroids in feces. The fraction also induces a triglyceride-lowering activity in the liver by decreasing hepatic lipogenesis.

Abbreviations

CPT

carnitine palmitoyltransferase

DHA

docosahexaenoic acid

EPA

eicosapentaenoic acid

FAS

fatty acid synthase

G6PDH

glucose-6-phosphate dehydrogenase

HDL

high density lipoprotein

PAP

phosphatidate phosphohydrolase

PUFA

polyunsaturated fatty acids

VLDL

very low density lipoprotein

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Copyright information

© AOCS Press 2001

Authors and Affiliations

  • Kazunari Tanaka
    • 3
  • Ikuo Ikeda
    • 1
  • Hiroko Yoshida
    • 2
  • Katsumi Imaizumi
    • 1
  1. 1.Laboratory of Nutrition Chemistry, Faculty of AgricultureGraduate School Kyushu UniversityFukuokaJapan
  2. 2.Division of Food and NutritionNakamura Junior CollegeFukuokaJapan
  3. 3.Laboratory of Nutrition ChemistrySiebold University of NagasakiNishisonogi Gun, Nagasaki Pref.Japan

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