Lipids

, 35:967

Lipid peroxidation as determined by plasma isoprostanes is related to disease severity in mild asthma

  • Lisa G. Wood
  • Dominic A. Fitzgerald
  • Peter C. Gibson
  • David M. Cooper
  • Manohar L. Garg
Article

DOI: 10.1007/s11745-000-0607-x

Cite this article as:
Wood, L.G., Fitzgerald, D.A., Gibson, P.C. et al. Lipids (2000) 35: 967. doi:10.1007/s11745-000-0607-x

Abstract

Oxidative stress is believed to play an important role in the pathophysiology of asthma. Recently discovered F2-isoprostanes, of which 8-iso-PGF is the most well-known isomer, have emerged as the most reliable marker of in vivo oxidative stress. The aim of this study was to examine 8-iso-PGF as a biomarker of oxidative stress in mild asthma in relation to endogenous and dietary antioxidant protection. Total (free and esterified) plasma 8-iso-PGF, plasma dietary antioxidants (vitamins E and C,β-carotene, Zn, and Se), and erythrocyte antioxidant enzyme activities (glutathione peroxidase and superoxide dismutase) were measured in 15 mild asthmatics and 15 age-and sex-matched controls. Total plasma 8-iso-PGF levels [median (quartile 1-quartile 3)] were significantly increased in the sthmatics [213 pg/mL (122–455) vs. 139 pg/mL (109–174), P=0.042]. The 8-iso-PGF levels were found to be associated with clinical asthma severity (P=0.044) and inhaled corticosteroid use (P=0.027) in asthmatics. No differences were observed in the plasma dietary antioxidant vitamins. The asthmatics had significantly lower plasma levels of Zn (P=0.027) and Se (P=0.006). Plasma Se correlated negatively with 8-iso-PGF (r=−0.725, P=0.002). No differences between the groups were observed for glutathione peroxidase or superoxide dismutase, however, superoxide dismutase activity was negatively associated with asthma severity (P=0.042). In conclusion, oxidative stress is increased in mild asthmatics, as reflected by increased plasma levels of 8-iso-PGF and a deficiency in plasma Zn and Se. The isoprostane 8-iso-PGF may provide a useful tool in intervention studies aimed at improving clinical status in asthma.

Abbreviations

BHT

butylated hydroxytoluene

EIA

enzyme immunoassay

FEV1

forced expiratory volume in one second

FVC

forced vital capacity

GC-MS

gas chromatography-mass spectrometry

GSHPx

glutathione peroxidase

Hb

hemoglobin

HPLC

high-performance liquid chromatography

TCP-MS

inductively coupled plasma-mass spectrometry

8-iso-PGF

isomer of F2-isoprostane

MDA

malondialdehyde

ROS

reactive oxygen species

SEM

standard error of the mean

SOD

superoxide dismutase

TBARS

thiobarbituric acid-reactive substance

TEAC

Trolox equivalent antioxidant capacity

TxB2

tritium-labeled thromboxane

Copyright information

© AOCS Press 2000

Authors and Affiliations

  • Lisa G. Wood
    • 1
  • Dominic A. Fitzgerald
    • 2
  • Peter C. Gibson
    • 3
  • David M. Cooper
    • 2
  • Manohar L. Garg
    • 1
  1. 1.Discipline of Nutrition and Dietetics Faculty of Medicine and Health SciencesUniversity of NewcastleNewcastleAustralia
  2. 2.Department of PaediatricsJohn Hunter Children's HospitalRankin ParkAustralia
  3. 3.Airway Research Centre, Department of Respiratory MedicineJohn Hunter HospitalRankin ParkAustralia
  4. 4.Department of Respiratory MedicineNew Children's HospitalParramattaAustralia

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