Journal of Surfactants and Detergents

, Volume 9, Issue 1, pp 69–75 | Cite as

Effect of temperature on the biodegradation of linear alkylbenzene sulfonate and alcohol ethoxylate

  • Daniel Prats
  • Carmen López
  • Diana Vallejo
  • Pedro Varó
  • Víctor M. León


The effect of temperature on the biodegradation of linear alkylbenzene sulfonate (LAS) and alcohol ethoxylate (AE) was evaluated using method OECD 303 A, Confirmatory test (Husmann units). The experiments were performed using an initial surfactant concentration of 10 mg/L and working temperatures of 25, 15, and 9°C, keeping the biodegradation units inside a thermostatic chamber. In all cases, the removal of both surfactants tested, LAS and AE, was higher than 90%, regardless of the temperature used in the test. We observed that longer acclimation periods were needed by the microorganisms at lower temperatures.

Key Words

Activated sludge alcohol ethoxylates biodegradation linear alkylbenzene sulfonate temperature 



alcohol ethoxylate


bismuth active substances


colony-forming units


dissolyed oxygen


dissolved organic carbon


ethylene oxide


high-performance liquid chromatography


linear alkylbenzene sulfonate


methylene-blue active substances


mixed liquor suspended solids


nonobserved effect concentration


Organisation for Economic Co-operation and Development


sludge retention time


wastewater treatment plant(s)


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  1. 1.
    Berna, J.L., and L. Cavalli, Linear Alkylbenzene Sulfonate, in Facts & Figures, European Council on Studies on LAB and LAS (ECOSOL), Australia, December 1999.Google Scholar
  2. 2.
    Berna, J.L., J. Ferrer, A. Moreno, D. Prats, and F. Ruiz Beviá, The Fate of LAS in the Environment, Tenside Surfact. Deterg. 26:101 (1989).Google Scholar
  3. 3.
    Kiewiet, A.T., J.R. Parsons, and H.A.J. Govers, Prediction of the Fate of Alcohol Ethoxylates in Sewage Treatment Plants, Chemosphere 34:1795 (1997).CrossRefGoogle Scholar
  4. 4.
    Matthijs, E., M.S. Holt, A. Kiewiet, and G.B. Rijs, Fate of Surfactants in Activated Sludge Wastewater Treatment Plants, Tenside Surfact. Deterg. 34:238 (1997).Google Scholar
  5. 5.
    Matthijs E., M.S. Holt, A. Kiewiet, and G.B. Rijs, Environmental Monitoring for LAS, AE, AES, AS, and Soap, Environ. Toxicol. Chem. 18:2634 (1999).CrossRefGoogle Scholar
  6. 6.
    Moreno, A., J. Ferrer, and J.L. Berna, Biodegradability of LAS in a Sewer System, Tenside Surfact. Deterg. 27:312 (1990).Google Scholar
  7. 7.
    Holt, M.S., K.K. Fox, M. Burford, M. Daniel, and H. Buckland, UK Monitoring Study on the Removal of Linear Alkylbenzene Sulphonate in Trickling Filter Type Sewage Treatment Plants, Contribution to GREAT-ER Project #2, Sci. Total Environ. 210/211:255 (1998).CrossRefGoogle Scholar
  8. 8.
    Field, J.A., T.P. Field, H. Siegrist, and W. Giger, Fate of Secondary Alkane Sulfonate Surfactants During Municipal Wastewater Treatment, Water Res. 29:1301 (1995).CrossRefGoogle Scholar
  9. 9.
    McAvoy, D.C., S.D. Dyer, N.J. Fendinger, W.S. Eckhoff, D.L. Lawrence, and W.M. Begley, Removal of Alcohol Ethoxylates, Alkyl Ethoxylate Sulfates, and Linear Alkylbenzene Sulfonates in Water Treatment, Environm. Toxicol. Chem. 17:1705 (1998).CrossRefGoogle Scholar
  10. 10.
    Trehy, M.L., W.E. Gledhill, J.P. Mierue, J.E. Adamove, A.M. Nielsen, H.O. Perkins, and W.S. Eckhoff, Environmental Monitoring for Linear Alkylbenzene Sulfonates, Dialkyltetralin Sulfonates and Their Biodegradation Intermediates, Environ. Toxicol. Chem. 15:233 (1996).CrossRefGoogle Scholar
  11. 11.
    Holt, M.S., M. Daniel, H. Buckland, and K.K. Fox, Monitoring Studies in the UK Designed for the Validation of the Geo-referenced Exposure Assessment Tool for European Rivers (GREAT-ER), in Proceedings of 5th World Cesio Congress, Firenze, Italy, 2000, p. 1358.Google Scholar
  12. 12.
    Cavalli, L., G. Cassani, and M. Lazzarin, Biodegradation of LAS and Alcohol Ethoxylate (AE). Application of Specific Analytical Methodologies, Tenside Surfact. Deterg. 33:158 (1996).Google Scholar
  13. 13.
    Cavalli, L., R. Clerici, P. Radici, and L. Valtorta, Update on LAB/LAS, Tenside Surfact. Deterg. 36:254 (1999).Google Scholar
  14. 14.
    Battersby, N.S., A.J. Sherren, R.N. Bumpus, R. Eagle, and I.K. Modale, The Fate of Linear Alcohol Ethoxylates During Activated Sludge Sewage Treatment, Chemosphere 45:109 (2001).CrossRefGoogle Scholar
  15. 15.
    HERA, Human and Environmental Risk Assessment of Linear Alkylbenzene Sulfonate, Cler Rev. 9:1 (2004).Google Scholar
  16. 16.
    García, M.T., E. Campos, M. Dalmau, I. Ribosa, and J. Sánchez-Leal, Structure-Activity Relationships for Association of Linear Alkylbenzene Sulfonates with Activated Sludge, Chemosphere 49:279 (2002).CrossRefGoogle Scholar
  17. 17.
    Games, L.M., Field Validation of Exposure Analysis Modelling System (EXAMS) in a Flowing Stream, in Modelling the Fate of Chemicals in the Aquatic Environment, edited by K.L. Dickson, A.W. Maki, and J. Cairns, Ann Arbor Science Publishers, Ann Arbor, MI, 1982, p. 325.Google Scholar
  18. 18.
    Swisher, R.D., Surfactant Biodegradation: Chemical Structure and Primary Biodegradation, Surfactant Science Series, Marcel Dekker, New York, 1987, Vol. 18, p. 415.Google Scholar
  19. 19.
    Gerike, P., and W. Jasiak, How Completely Are Surfactants Biodegraded? Tenside Deterg. 23:300 (1986).Google Scholar
  20. 20.
    Kravetz, L., H. Chung, K.F. Guin, W.T. Shebs, and L.S. Smith, Primary and Ultimate Biodegradation of an Alcohol Ethoxylate and a Nonylphenol Ethoxylate Under Average Winter Conditions in the United States, Tenside Deterg. 21:1 (1984).Google Scholar
  21. 21.
    Verstraete, W., and E. van Vaerenberg, Aerobic Activated Sludge, in Biotechnology, edited by H.J. Rehm and G. Reed, Microbial Degradations Series, VCH, Weinheim, 1986, Vol. 8, p. 43.Google Scholar
  22. 22.
    Birch, R.R., Prediction of the Fate of Detergent Chemicals During Sewage Treatment, J. Chem. Technol. Biotechnol. 50:411 (1991).CrossRefGoogle Scholar
  23. 23.
    Organisation for Economic Co-operation and Development (OECD), Simulation Test—Aerobic Sewage Treatment 303 A, OECD, Paris, 1996.Google Scholar
  24. 24.
    American Public Health Association, AWWA & APHA Standard Methods for the Examination of Water and Wastewater, 20th edn., American Public Health Association (APHA), American Water Works Association (AWWA), and Water Pollution Control Federation (WPCF), Washington, DC, 1998.Google Scholar
  25. 25.
    Eaton, A.D., L.S. Clesceri, and A.E. Greenberg (eds.), Standards Methods for the Examination of Water and Wastewater, 20th edn., American Public Health Association, Washington, DC, 1998.Google Scholar
  26. 26.
    European Economic Community (EEC), Directive 82/243/EEC, DO L 109 (1982).Google Scholar
  27. 27.
    Matthijs, E., and H. De Henau, Determination of Linear Alkylbenzene Sulfonates in Aqueous Samples Sediments, Sludges and Soils Using HPLC, Tenside Surfact. Deterg. 24:193 (1987).Google Scholar
  28. 28.
    Wickbold, R., Zur Bestimmung nichtionischer Tenside Fluss und Abwasser, Tenside Surfact. Deterg, 9:173 (1972).Google Scholar
  29. 29.
    Water, J., and F. Longman, A Colorimetric Modification of the Wickbold Procedure for the Determination of Non-ionic Surfactants in Biodegradation Test Liquors, Anal. Chim. Acta 93:341 (1977).CrossRefGoogle Scholar
  30. 30.
    Water, J., J. Garrigan, and A. Paulson, Investigations into the Scope and Limitations of the Bismuth Active Substances Procedure (Wickbold) for the Determination of Non-ionic Surfactants in Environmental Samples, Water Res. 20:247 (1986).CrossRefGoogle Scholar
  31. 31.
    Tian, S., L. Lishman, and K.L. Murphy, Investigations into Excess Activated Sludge Accumulation at Low Temperatures, Water Res. 28:501 (1994).CrossRefGoogle Scholar
  32. 32.
    International Organization for Standardization (ISO). Water Quality—Evaluation of Elimination and Biodegradability of Organic Compounds in an Aqueous Medium—Activated Sludge Simulation Test, ISO, Geneva, Switzerland, 1995, ISO Standard 11733.Google Scholar
  33. 33.
    León, V.M., and E. González-Mazo, Biodegradation of Linear Alkylbenzene Sulfonates in the Marine Environment, in Analysis and Fate of Surfactants in the Aquatic Environment, edited by T. Knepper, D. Barceló, and P. de Vogt, Comprehensive Analytical Chemistry Series 40, Elsevier Science, Dordrecht, The Netherlands, 2003, p. 591.Google Scholar
  34. 34.
    Moreno, A., J. Ferrer, J. Bravo, J.L. Berna, and L. Cavalli, Effect of the Alkyl Chain and Concentration of Linear Alkylbenzene Sulfonate on Biodegradation Kinetics, Tenside Surfact. Deterg. 35:375 (1998).Google Scholar

Copyright information

© AOCS Press 2006

Authors and Affiliations

  • Daniel Prats
    • 1
  • Carmen López
    • 1
  • Diana Vallejo
    • 1
  • Pedro Varó
    • 1
  • Víctor M. León
    • 1
  1. 1.Department of Chemical EngineeringAlicante UniversityAlicanteSpain

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