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Acta Physiologiae Plantarum

, Volume 21, Issue 2, pp 127–132 | Cite as

Role of spermidine in the stabilization of the apoprotein of the light-harvesting chlorophyll a/b-protein complex of photosystem II during leaf senescence process

  • Jolanta Legocka
  • Irena Zajchert
Article

Abstract

Barley leaf discs maintained in dark accumulated a massive amount of putrescine (Put), lost chlorophyll and senescenced rapidly. At the same time RNase activity increased significantly. Exogenous spermidine (Spd) inhibited RNase activity, the loss of chlorophyll and degradation of the proteins from thylakoid membranes. Using SDS-PAGE and immunoblot analysis it was shown that spermidine was effective in the retardation of the loss of LHCPII observed in water-treated detached leaves. Analysis of PSII particles isolated from leaf fragments floated in water in the dark revealed the presence of Put, Spd and Spm. In spermidine treated leaves the level of this polyamine in photosystem II was above 5-fold higher than in control. The experimental findings obtained in this study provide evidence that applied spermidine interacts directly with thylakoid membranes so that they become more stable to degradation during senescence.

Key words

barley LHCPII photosystem II senescence spermidine thylakoid membrane proteins 

List of abbreviations

Chl

chlorophyll

LHCII

light harvesting chlorophyll a/b-protein complex

LHCPII

apoprotein of the light-harvesting chlorophyll a/b-protein complex of photosystem II

PAs

polyamines

PCA

perchloric acid

PSII

photosystem II

Put

putrescine

Spd

spermidine

Spm

spermine

SDS-PAGE

sodium dodecylsulphate polyacrylamide gel electrophoresis

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Copyright information

© Department of Plant Physiology 1999

Authors and Affiliations

  • Jolanta Legocka
    • 1
  • Irena Zajchert
    • 1
  1. 1.Laboratory of Plant Physiology, Faculty of BiologyAdam Mickiewicz UniversityPoznań

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