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Acta Physiologiae Plantarum

, Volume 20, Issue 3, pp 229–233 | Cite as

Chromatographic properties of the cadmium induced SOD isoform in Rhizopogon roseolus

  • Zbigniew Miszalski
  • Ewa Niewiadomska
  • Andrzej Skoczowski
  • Bernard Botton
Article

Abstract

Exposure of Rhizopogon roseolus mycelia to 15 mmol·dm−3 cadmium for 24 h induces a different pattern of Mn-SOD on polyacrylamide gels, probably being a changed form of an originally existing one. The presence of cadmium affects the chromatographic properties of this enzyme and its mobility through the acrylamide gel. This new isoform was purified using DEAE Trisacryl chromatography. Cadmium induced isoform adsorbed stronger to the ligands and was eluated with a Tris-HCl buffer containing 0.1 mol·dm−3 NaCl. SOD from control samples (not treated with cadmium) was eluated with the same buffer without NaCl.

Keywords

cadmium SOD Rhizopogon roseolus 

List of abbreviations

DEAE

diethylaminoethyl

NBT

nitro blue tetrazolium

PAGE

polyacrylamide gel electrophoresis

SOD

superoxide dismutase [1.15.1.1]

TEMED

(N,N,N,N,′-tetramethylethylene-diamine), Tris-tris(hydroxymethyl)aminomethane

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Copyright information

© Department of Plant Physiology 1998

Authors and Affiliations

  • Zbigniew Miszalski
    • 1
  • Ewa Niewiadomska
    • 1
  • Andrzej Skoczowski
    • 2
  • Bernard Botton
    • 3
  1. 1.The Franciszek Górski Department of Plant PhysiologyPolish Academy of SciencesCracowPoland
  2. 2.The Franciszek Górski Department of Plant PhysiologyPolish Academy of SciencesCracowPoland
  3. 3.Laboratoire de Biologie Forestière, associè INRAUniversité Henri Poincaré Nancy IVandoeuvre-les-Nancy CedexFrance

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