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Acta Physiologiae Plantarum

, 42:20 | Cite as

Grain protein content comparison and proteomic analysis of foxtail millet (Setaria italica L.) seed response to different drought stress levels

  • Binqin Xu
  • Xiaoli Gao
  • Kongjun Dong
  • Xia Li
  • Pu Yang
  • Tianyu Yang
  • Baili FengEmail author
Original Article
  • 21 Downloads

Abstract

As one of the main indicators of nutritional values of foxtail millet, grain protein contents (GPCs) of seeds can be affected by soil water conditions. Studies in this report indicated that intensified drought in filling stage increased the GPCs. To gain better insight into the interaction mechanism between the GPCs and soil water deficits, the proteomic profiles of mature seeds under three drought stress levels (moderate drought-W2; combined drought-W1; severe drought-W0) were conducted on two varieties, Longgu6 (LG6) and Huangjinmiao (HJM) by two-dimensional electrophoresis, with acidic (pH 4–7) gels. There were 83 protein spots in total identified successfully in two comparison groups (W1 vs W2 and W0 vs W2), associated with four major biochemical and metabolic pathways, including storage protein, amino acid and protein metabolisms, energy metabolism, response to stress and defense, and chaperones. Further analysis revealed that the increase of GPCs in LG6 under both W0 and W1 may be owing to the up-regulated storage proteins and the up-regulated protein response to drought stress. The levels of DEPs related to protein and amino acid biosynthesis and starch metabolism increased greatly in LG6 under W1, and this may also result in the high GPC level of seeds in LG6 under W1. Protein biosynthesis was up-regulated and drought stress-related pathways were enhanced in HJM under W1, thereby resulting in the increase of GPC in HJM under W1. These results will contribute to the existing knowledge on the complexity of protein changes leading to the GPC changes in response to drought.

Keywords

Drought stress Grain protein content Foxtail millet Two-dimensional electrophoresis Differentially expressed protein 

Notes

Acknowledgement

This study was supported by China Agriculture Research System (CARS-06), the National Natural Science Foundation of China (31371529), and the Minor Grain Crops Research and Development System of Shaanxi Province (2014–2017).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interests.

Supplementary material

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2020

Authors and Affiliations

  1. 1.State Key Laboratory of Crop Stress Biology in Arid AreasA&F UniversityYanglingChina
  2. 2.Gansu Academy of AgriculturalLanzhouChina

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