Acta Physiologiae Plantarum

, 41:179 | Cite as

An orthogonal test of the effect of NO3, PO43−, K+, and Ca2+ on the growth and ion absorption of Elaeagnus angustifolia L. seedlings under salt stress

  • Lingxiao Li
  • Tingting Zhu
  • Jing Liu
  • Chen Zhao
  • Lingyu Li
  • Min ChenEmail author
Original Article


Excess salts in soil severely inhibit plant growth and yield. Various nutrients can relieve the inhibitory effect of salt on plants. Elaeagnus angustifolia is a tree species that is utilized in landscaping and ecological restoration in China and shows tolerance to saline soils. In this experiment, we first determined the optimal concentration of single-nutrient inputs for promoting growth of E. angustifolia seedlings and alleviating salt stress. The optimal nitrogen (NO3), phosphorus (PO43−), potassium (K+), and calcium (Ca2+) concentrations under salt stress were 2, 0.6, 2, and 2 mM, respectively. Based on this result, we carried out an optimum nutrient ratio experiment by a four-factor and three-level orthogonal experiment. The results showed that under normal growth conditions, the optimal nutrient combinations for NO3, PO43−, K+, and Ca2+ were 2, 0.6, 5, and 1 mM; 5, 0.2, 5, and 2 mM; and 5, 0.6, 1, and 5 mM, respectively, which increased plant height, biomass, and the chlorophyll content of E. angustifolia seedlings. Under salt stress, the optimal nutrient combinations were 5, 0.2, 5, and 2 mM; and 5, 0.6, 1, and 5 mM, respectively. This study provides a theoretical basis for optimal fertilization of E. angustifolia seedlings in saline-alkali soils.


Elaeagnus angustifolia L. Orthogonal test Salt stress Nutrient 



This work was supported by the major projects of science and technology in Shandong province (2017CXGC0311, 2017CXGC0313) and the Program for Scientific Research Innovation Team in Colleges and Universities of Shandong Province.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.


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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2019

Authors and Affiliations

  • Lingxiao Li
    • 1
  • Tingting Zhu
    • 1
  • Jing Liu
    • 1
  • Chen Zhao
    • 1
  • Lingyu Li
    • 1
  • Min Chen
    • 1
    Email author
  1. 1.Shandong Provincial Key Laboratory of Plant Stress Research, College of Life ScienceShandong Normal UniversityJinanPeople’s Republic of China

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