Mitigation of As toxicity in wheat by exogenous application of hydroxamate siderophore of Aspergillus origin
Siderophores are secondary metabolites having molecular weight less than 10 KD. They are specifically meant for chelation of ferric ions. They also tend to chelate metals under heavy metal stress, thus reducing their toxic effects. In the current study, experiments have been conducted on wheat plants to analyse siderophore’s ability to counteract the adverse impact of arsenic (As) toxicity on physiology of plant seedlings along with biochemical response. As toxicity has been observed to adversely affect the lengths of root and shoot, chlorophyll and carotenoid contents, and activities of various antioxidative enzymes. The present study revealed that the application of hydroxamate-type siderophore isolated from Aspergillus nidulans under toxic condition significantly recovered the growth and helped in amending the enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) of wheat genotype (NW1014). At the same time, injury caused by lipid peroxidation was significantly reduced. In silico studies revealed better binding affinity of ferricrocin–arsenate complex leading to thermodynamically stable complex. Encouraging results of As containment by organic biomolecule-siderophore can lead to an emerging bioremediation mechanism brimming with opportunities for agricultural field and environmental clean-up.
KeywordsSiderophore As stress Triticum aestivum Hydroxamate Aspergillus nidulans
Authors Azmi Khan and Pratika Singh are obliged to the UGC, New Delhi for financial support in the form of fellowship.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Aebi H (1984) Catalase in vitro. Methods in enzymology, vol 105. Academic Press, Florida, pp 114–121Google Scholar
- Gusain P, Singh V (2016) Hydroxamate and catecholate siderophore synthesizing As resistant pgpr. J Environ Appl Biores 4:01–04Google Scholar
- Hoagland DR, Arnon DI (1938) The water-culture method for growing plants without soil. Circ Calif Agric Exp Sta 347:1–35Google Scholar
- Morton WE, Dunnette DA (1994) Health effects of environmental As. In: Nriagu JO (ed) As in the environment. Part II. Human health and ecosystem effects. Wiley, New York, pp 17–34Google Scholar
- Olsen SR, Cole CV, Watanabe FS, Dean LA (1954) Estimation of available phosphorus in soils by extraction with sodium bicarbonate. US Department of Agric. Circ p. 939Google Scholar
- Puschenreiter M, Gruber B, Wenzel WW, Schindlegger Y, Hann S, Spangl B, Schenkeveld WDC, Kraemer SM, Oburger E (2017) Phytosiderophore-induced mobilization and uptake of Cd, Cu, Fe, Ni, Pb and Zn by wheat plants grown on metal enriched soils. Environ Exp Bot 138:67–76. https://doi.org/10.1016/j.envexpbot.2017.03.011 CrossRefGoogle Scholar
- Stoeva N, Bineva T (2003) Oxidative changes and photosynthesis in oat plants grown in As-contaminated soil. Bulg J Plant Physiol 29:87–95Google Scholar
- Zhang FQ, Wang YS, Lou ZP, Dong JD (2007) Effect of heavy metal stress on antioxidative enzymes and lipid peroxidation in leaves and roots of two mangrove plant seedlings (Kandelia candel and Bruguiera gymnorrhiza). Chemosphere 67:44–50. https://doi.org/10.1016/j.chemosphere.2006.10.007 CrossRefGoogle Scholar
- Zhang WD, Liu DS, Tian JC, He FL (2009) Toxicity and accumulation of As in wheat (Triticum aestivum L.) varieties of China. Phyton (Buenos Aires) 78:147–154Google Scholar