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Mitigation of As toxicity in wheat by exogenous application of hydroxamate siderophore of Aspergillus origin

  • Sneha Kumari
  • Azmi Khan
  • Pratika Singh
  • Sharad Kumar Dwivedi
  • Krishna Kumar Ojha
  • Amrita SrivastavaEmail author
Original Article

Abstract

Siderophores are secondary metabolites having molecular weight less than 10 KD. They are specifically meant for chelation of ferric ions. They also tend to chelate metals under heavy metal stress, thus reducing their toxic effects. In the current study, experiments have been conducted on wheat plants to analyse siderophore’s ability to counteract the adverse impact of arsenic (As) toxicity on physiology of plant seedlings along with biochemical response. As toxicity has been observed to adversely affect the lengths of root and shoot, chlorophyll and carotenoid contents, and activities of various antioxidative enzymes. The present study revealed that the application of hydroxamate-type siderophore isolated from Aspergillus nidulans under toxic condition significantly recovered the growth and helped in amending the enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) of wheat genotype (NW1014). At the same time, injury caused by lipid peroxidation was significantly reduced. In silico studies revealed better binding affinity of ferricrocin–arsenate complex leading to thermodynamically stable complex. Encouraging results of As containment by organic biomolecule-siderophore can lead to an emerging bioremediation mechanism brimming with opportunities for agricultural field and environmental clean-up.

Keywords

Siderophore As stress Triticum aestivum Hydroxamate Aspergillus nidulans 

Notes

Acknowledgements

Authors Azmi Khan and Pratika Singh are obliged to the UGC, New Delhi for financial support in the form of fellowship.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2019

Authors and Affiliations

  • Sneha Kumari
    • 1
  • Azmi Khan
    • 1
  • Pratika Singh
    • 1
  • Sharad Kumar Dwivedi
    • 2
  • Krishna Kumar Ojha
    • 3
  • Amrita Srivastava
    • 1
    Email author
  1. 1.Department of Life Science, School of Earth, Biological and Environmental SciencesCentral University of South BiharGayaIndia
  2. 2.ICAR Research Complex for Eastern RegionPatnaIndia
  3. 3.Department of BioinformaticsCentral University of South BiharGayaIndia

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