One of the most important stresses imperiling plant production in arid and semi-arid areas is salinity. By slow pyrolysis, a solid organic material, which is called biochar (BC), has been produced from waste organic substances. In this research, a pot factorial arrangement on the basis of randomized complete design was accomplished to evaluate the influence of BC on some physiological traits and growth at two different growth stages of summer savory (Satureja hortensis L.) under salinity stress. The treatments were included of three levels of BC (0, 1 and 2% w/w in the soil) and four salinity levels (0, 40, 80 and 120 mM of NaCl) with four replications. According to the findings, application of BC, especially at 2% w/w in the soil under salinity stress, reduced electrolyte leakage (EL) and antioxidant enzyme activities, i.e., ascorbate peroxidase (APX), catalase (CAT), superoxide dismutase (SOD), guaiacol peroxidase (GPX) activities and MDA content at vegetative and flowering stages. On the other side, the highest amounts of biomass and water content were observed when using BC 2% w/w of soil without salinity stress. The results confirmed that BC usage can contribute to the protection of the summer savory against salinity stress by alleviating the oxidative stress.
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Reactive oxygen species
Akbari S, Kordi S, Fatahi S, Ghanbari F (2013) Physiological responses of summer savory (Satureja hortensis L.) under salinity stress. Int J Agric Crop Sci 5(15):1702–1708
Akhtar SS, Andersen MN, Liu F (2015) Residual effects of biochar on improving growth, physiology and yield of wheat under salt stress. Agric Water Manag 158:61–68
Ali S, Rizwan M, Qayyum MF, Ok YS, Ibrahim M, Riaz M, Arif MS, Hafeez F, Al-Wabel MI, Shahzad AN (2017) Biochar soil amendment on alleviation of drought and salt stress in plants: a critical review. Environ Sci Pollut Res 24(14):12700–12712
Attia H, Arnaud N, Karray N, Lachaâl M (2008) Long-term effects of mild salt stress on growth, ion accumulation and superoxide dismutase expression of Arabidopsis rosette leaves. Physiol Plant 132:293–305
Bączek-Kwinta R (2017) Swailing affects seed germination of plants of European bio-and agricenosis in a different way. Open Life Sci 12(1):62–75
Beers RF, Sizer IW (1952) A spectrophotometric method of measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–140
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254
Cheraghi SAM (2004) Institutional and scientific profiles of organizations working on saline agriculture in Iran. In: Prospects of saline agriculture in the Arabian Peninsula: proceedings of the international seminar on prospects of saline agriculture in the GCC countries 18–20 March 2001, Dubai, United Arab Emirates, Taha FK, Ismail S, Jaradat A (eds). Amherst Scientific Publishers: Amherst, pp 399–412
Delavari P, Baghizadeh A, Enteshari S, Kalantari KM, Yazdanpanah A, Mousavi E (2010) The effects of salicylic acid on some of biochemical and morphological characteristic of Ocimum basilicucm under salinity stress. Aust J Basic Appl Sci 4(10):4832–4845
English JP, Colmer TD (2013) Tolerance of extreme salinity in two stem-succulent halophytes (Tecticornia species). Funct Plant Biol 40:897–912
Farhangi-Abriz S, Torabian S (2017) Antioxidant enzyme and osmotic adjustment changes in bean seedlings as affected by biochar under salt stress. Ecotoxicol Environ Saf 137:64–70
Flowers TJ, Galal HK, Bromham L (2010a) Evolution of halophytes: multiple origins of salt tolerance in land plants. Funct Plant Biol 37:604–612
Flowers TJ, Gaur PM, Gowda CLL, Krishnamurthy L, Samineni S, Siddique KH, Tuner NC, Vadez V, Varshney RK, Colmer TD (2010b) Salt sensitivity in chickpea. Plant Cell Environ 33:490–509
Fu J, Huang B (2001) Involvement of anti-oxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environ Exp Bot 45:105–114
Gapińska M, Skłodowska M, Gabara B (2008) Effect of short-and long-term salinity on the activities of antioxidative enzymes and lipid peroxidation in tomato roots. Acta Physiol Plant 30(1):11–18
Giannopolitis CN, Ries SK (1977) Superoxide dismutases: I. Occurrence in higher plants. Plant Physiol 59(2):309–314
Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48(12):909–930
Haddadi BS, Hassanpour H, Niknam V (2016) Effect of salinity and waterlogging on growth, anatomical and antioxidative responses in Mentha aquatica L. Acta Physiol Plant 38(5):119–125
Hu L, Li H, Pang H, Fu J (2012) Responses of antioxidant gene, protein and enzymes to salinity stress in two genotypes of perennial ryegrass (Lolium perenne) differing in salt tolerance. J Plant Physiol 169(2):146–156
Jakovljević DZ, Topuzović MD, Stanković MS, Bojović BM (2017) Changes in antioxidant enzyme activity in response to salinity-induced oxidative stress during early growth of sweet basil. Hortic Environ Biotechnol 58(3):240–246
Jbir-Koubaa R, Charfeddine S, Ellouz W, Saidi MN, Drira N, Gargouri-Bouzid R, Nouri-Ellouz O (2015) Investigation of the response to salinity and to oxidative stress of interspecific potato somatic hybrids grown in a greenhouse. Plant Cell Tissue Organ Cult 120(3):933–947
Jebara S, Jebara M, Limam F, Aouani ME (2005) Changes in ascorbate peroxidase, catalase, guaiacol peroxidase and superoxide dismutase activities in common bean (Phaseolus vulgaris) nodules under salt stress. J Plant Physiol 162(8):929–936
Jia L, Xu W, Li W, Ye N, Liu R, Shi L, Bin Rahman AR, Fan M, Zhang J (2013) Class III peroxidases are activated in proanthocyanidin-deficient Arabidopsis thaliana seeds. Ann Bot 111(5):839–847
Khan MN, Siddiqui MH, Mohammad F, Naeem M, Khan MMA (2010) Calcium chloride and gibberellic acid protect linseed (Linum usitatissimum L.) from NaCl stress by inducing antioxidative defense system and osmoprotectant accumulation. Acta Physiol Plant 32:121–132
Kim HS, Kim KR, Yang JE, Ok YS, Owens G, Nehls T, Wessolek G, Kim KH (2016) Effect of biochar on reclaimed tidal land soil properties and maize (Zea mays L.) response. Chemosphere 142:153–159
Liu ZJ, Guo Y, Bai JG (2010) Exogenous hydrogen peroxide changes antioxidant enzyme activity and protects ultrastructure in leaves of two cucumber ecotypes under osmotic stress. Plant Growth Regul 29(2):171–183
Momtaz S, Abdollahi M (2010) An update on pharmacology of Satureja species; from antioxidant, antimicrobial, antidiabetes and anti-hyperlipidemic to reproductive stimulation. Int J Pharmacol 6(4):346–353
Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22(5):867–880
Nelissen V, Rutting T, Huygens D, Staelens J, Ruysschaert G, Boeckx P (2012) Maize biochars accelerate short-term soil nitrogen dynamics in a loamy sand soil. Soil Biol Biochem 55:20–27
Prommer J, Wanek W, Hofhansl F, Trojan D, Offre P, Urich T, Schleper C, Sassmann S, Kitzler B, Soja G, Hood-Nowotny RC (2014) Biochar decelerates soil organic nitrogen cycling but stimulates soil nitrification in a temperate arable field trial. PLoS One 9(1):86388–86400
Rajapaksha AU, Chen SS, Tsang DC, Zhang M, Vithanage M, Mandal S, Gao B, Bolan NS, Ok YS (2016) Engineered/designer biochar for contaminant removal/immobilization from soil and water: potential and implication of biochar modification. Chemosphere 148(27):276–291
Rizwan M, Ali S, Qayyum MF, Ibrahim M, Zia-ur-Rehman M, Abbas T, Ok YS (2016) Mechanisms of biochar-mediated alleviation of toxicity of trace elements in plants: a critical review. Environ Sci Pollut Res 23(3):2230–2248
Sheng M, Tang M, Chan H, Yang B, Zhang F, Huang Y (2008) Influence of arbuscular mycorrhizae on photosynthesis and water status of maize plants under salt stress. Mycorrhiza 18:287–296
Sohi SP, Krull E, Lopez-Capele E, Bol R (2010) A review of biochar and its use and function in soil. Adv Agron 105:47–82 (Chapter 2)
Soleimani Z, Afshar A, Nematpour F (2017) Responses of antioxidant gene and enzymes to salinity stress in the Cuminum cyminum L. Russ J Plant Physiol 64(3):361–367
Stewart RR, Bewley JD (1980) Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiol 65(2):245–248
Taarit MB, Msaada K, Hosni K, Marzouk B (2011) Physiological changes and essential oil composition of clary sage (Salvia sclarea L.) rosette leaves as affected by salinity. Acta Physiol Plant 33(1):153–162
Thomas SC, FryeS Gale N, Garmon M, Launchbury R, Machado N, Melamed S, Murray J, Petroff A, Winsborough C (2013) Biochar mitigates negative effects of salt additions on two herbaceous plant species. J Environ Manag 129:62–68
Torabi S, Niknam V (2011) Effects of iso-osmotic concentrations of NaCl and mannitol on some metabolic activity in calluses of two Salicornia species. Vitro Cell Dev Biol Plant 47(6):734–742
Unal BT, Aktas L, Guven A (2014) Effects of salinity on antioxidant enzymes and proline in leaves of barley seedlings in different growth stages. Bulg J Agric Sci 20(4):883–887
Waling I, Van W, Vark VJG, Houba JJ, Van Der Lee L (1989) Soil and plant analysis, a series of syllabi. Part 7. Plant analysis procedures. Wageningen Agriculture University, Wageningen
Wang W, Vinocur B, Altman A (2003) Plant responses to drought, salinity and extreme temperatures: toward genetic engineering for stress tolerance. Planta 218:1–14
Wu GQ, Wang SM (2012) Calcium regulates K+/Na+ homeostasis in rice (Oryza sativa L.) under saline conditions. Plant Soil Environ 58:121–127
Yamauchi Y, Furutera A, Seki K, Toyoda Y, Tanaka K, Sugimoto Y (2008) Malondialdehyde generated from peroxidized linolenic acid causes protein modification in heat-stressed plants. Plant Physiol Biochem 46(8):786–793
Yue LJ, Ma Q, Li SX, Zhou XR, Wu GQ, Bao AK, Zhang JL, Wang SM (2012) NaCl stimulates growth and alleviates water stress in the xerophyte Zygophyllum xanthoxylum. J Arid Environ 87:153–160
This research was supported by Ferdowsi University of Mashhad, Iran. The authors appreciate Lucia Marone from McGill University because of editing the manuscript as a native English speaker.
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Communicated by R. Baczek-Kwinta.
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Mehdizadeh, L., Moghaddam, M. & Lakzian, A. Alleviating negative effects of salinity stress in summer savory (Satureja hortensis L.) by biochar application. Acta Physiol Plant 41, 98 (2019). https://doi.org/10.1007/s11738-019-2900-3
- Salt stress
- Antioxidant enzyme activities
- Electrolyte leakage