Somatic embryogenesis-mediated plant regeneration of Eclipta alba (L.) Hassk. and its conservation through synthetic seed technology
- 7 Downloads
Plant regeneration by means of somatic embryogenesis has been standardized for the first time in Eclipta alba (L.) Hassk. Explants like nodal segment, shoot tip, and leaf were tested on Murashige and Skoog medium supplemented with picloram, 2,4-dichlorophenoxyacetic acid, or α-naphthalene acetic acid for callus induction. Nodal segment exhibited the maximum response (92.80%) to callus induction on culture medium-containing 2.0 mg l−1 picloram. Furthermore, highest rate (88.40%) and number (34.83) of somatic embryo induction were found on medium fortified with 2.0 mg l−1 picloram, 0.5 mg l−1 thidiazuron, and 0.25 mg l−1 abscisic acid. Influence of abscisic acid on the somatic embryo regeneration was also tested with the highest frequency (95.50%) attained on medium-containing 0.75 mg l−1 N6-benzyladenine and 0.5 mg l−1 abscisic acid. Somatic embryo of torpedo stage was efficiently encapsulated with 2.5% sodium alginate and 75 mM calcium chloride, and exhibited 93.33% germination rate. The study revealed that storage at low temperature (8 °C) gave superior results where 86.67% of the synthetic seeds sprouted even after 60 days with a decline to 46.67% in 90 days post-storage. The rate of germination at 25 °C had been constantly low and failed to sprout after 75 days. The acclimatization of the regenerated plantlets was successfully taken place in garden soil, sand, and vermicompost in the ratio of 1:1:1 with 95% survival rate.
KeywordsAbscisic acid Eclipta alba Somatic embryogenesis Synthetic seed
Murashige and Skoog (1962)
α-Naphthalene acetic acid
Plant growth regulator
N-Phenyl-N′-(1,2,3-thidiazol-5-yl) urea or Thidiazuron
Authors acknowledge the laboratory, as well as library assistance from the Bidhan Chandra Krishi Viswavidyalaya, West Bengal, India.
This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Compliance with ethical standards
Conflict of interest
The authors declare that there are no conflicts of interest.
- Bhojwani SS, Razdan MK (1996) Plant tissue culture: theory and practice. Elsevier, AmsterdamGoogle Scholar
- Devendra BN, Srinivas N, Sandeep Reddy A (2011) High frequency somatic embryogenesis and plant regeneration in nodal explant cultures of Eclipta alba L. Hassk. Ann Biol Res 2:143–149Google Scholar
- Evans DE, Coleman JOD, Kearns A (2003) Callus cultures. In: Basics plant tissue culture. BIOS Scientific Publishers, New YorkGoogle Scholar
- Gantait S, Kundu S, Ali MN (2015a) Influence of encapsulating agent and matrix levels on synseed production of Bacopa monnieri (L.) Pennell. Med Plants 7:182–187Google Scholar
- Gantait S, Kundu S, Yeasmin L, Ali N (2017) Impact of differential levels of sodium alginate, calcium chloride and basal media on germination frequency of genetically true artificial seeds of Rauvolfia serpentina (L.) Benth. ex Kurz. J Appl Res Med Aromat Plants 4:75–81Google Scholar
- Kumar V, Chandra S (2014) High frequency somatic embryogenesis and synthetic seed production of the endangered species Swertia chirayita. Biology 69:186–192Google Scholar
- Micheli M, Standardi A (2016) From somatic embryo to synthetic seed in Citrus spp. through the encapsulation technology. In: Germanà MA, Lambardi M (eds) In vitro embrogenesis in higher plants methods in molecular biology. Springer, Heidelberg, pp 515–522Google Scholar
- Nirala NK, Das DK, Reddy MK, Srivastava PS, Sopory SK, Upadhyaya KC (2010) Encapsulated somatic embryos of grape (Vitis vinifera L.): an efficient way for storage, transport and multiplication of pathogen free plant material. Asia Pac J Mol Biol Biotechnol 18:159–162Google Scholar
- Ozudogru EA, Lambardi M (2016) Cryotechniques for the long-term conservation of embryogenic cultures from woody plants. In: Germanà MA, Lambardi M (eds) In vitro embrogenesis in higher plants. Methods in molecular biology. Springer, Heidelberg, pp 537–550Google Scholar
- Prakash P, Sharumathy D, Sunkar S, Nandagopal D, Narendrakumar G (2015) Micropropagation of E. alba using humic acid as media component. Plant Arch 15:181–185Google Scholar
- Salma U, Kundu S, Ali MN, Mandal N (2017) An efficient micropropagation protocol for Eclipta alba (L.) Hassk.: an endangered, medicinally important plant. J Crop Weed 13:49–54Google Scholar
- Satish L, Rency AS, Rathinapriya P, Ceasar SA, Pandian S, Rameshkumar R, Rao TB, Balachandran SM, Ramesh M (2015) Influence of plant growth regulators and spermidine on somatic embryogenesis and plant regeneration in four Indian genotypes of finger millet (Eleusine coracana (L.) Gaertn). Plant Cell Tissue Org Cult 10:100. https://doi.org/10.1007/s11240-015-0870-8 CrossRefGoogle Scholar
- Sharan AK, Dubey SR, Kumar R, Chandra V, Singh BP, Kumar G, Kumari S (2014) A novel protocol for propagation of Eclipta alba (L) Hassak. Int J Pure Appl Biosci 2:137–141Google Scholar
- Vahdati K, Jariteh M, Niknam V, Mirmasoumi M, Ebrahimzadeh H (2006) Somatic embryogenesis and embryo maturation in Persian walnut. Acta Hortic 705:199–205Google Scholar