Morpho-physiological and biochemical changes in black gram (Vigna mungo L. Hepper) genotypes under drought stress at flowering stage

  • S. Gurumurthy
  • Basudeb SarkarEmail author
  • M. Vanaja
  • Jyoti Lakshmi
  • S. K. Yadav
  • M. Maheswari
Original Article


The response of drought stress on morpho-physiological and biochemical characters was assessed in black gram genotypes in a pot culture experiment. Water stress was applied at flowering stage of the crop and various morpho-physiological and biochemical characters were analyzed under control and water stress conditions. The genotypes, water levels and their interaction varied significantly for majority of the traits quantified revealing the presence of substantial genetic diversity. Based on these studies, genotypes PGRU95016, COBG05, IPU99209, IPU941 and IPU243 were identified as tolerant to drought stress conditions. Photosynthesis, stomatal conductance, transpiration rate, total chlorophyll, proline content and peroxidase activity could be useful to screen for drought tolerance in black gram.


Black gram Biochemical Morphological and physiological traits Drought stress Genetic diversity 



The research was carried out under National Innovations on Climate Resilient Agriculture (NICRA) Project at Central Research Institute for Dryland Agriculture (CRIDA). Authors are thankful to the Project Coordinator, MULLaRP, Indian Institute for Pulses Research (IIPR), Kanpur, India for providing the seed material of genotypes used in the present investigation.


  1. Anjum SA, Xie X, Wang L, Saleem MF, Man C, Lei W (2011) Morphological, physiological and biochemical responses of plants to drought stress. Afr J Agric Res 6:2026–2032Google Scholar
  2. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15PubMedPubMedCentralGoogle Scholar
  3. Baburai Nagesh AK (2006) The physiological and genetic bases of water use efficiency in winter wheat. PhD Thesis, School of Biosciences, University of Nottingham, UKGoogle Scholar
  4. Baroowa B, Gogoi N (2012) Effect of induced drought on different growth and biochemical attributes of black gram (Vigna mungo L.) and green gram (Vigna radiata L.). J Env Res Dev 6:584–593Google Scholar
  5. Baroowa B, Gogoi N (2013) Biochemical changes in two Vigna sp. during drought and subsequent recovery. Indian J Plant Physiol 18:319–325Google Scholar
  6. Baroowa B, Gogoi N, Farooq M (2016) Changes in physiological, biochemical and antioxidant enzyme activities of green gram (Vigna radiata L.) genotypes under drought. Acta Physiol Plant 38:219.
  7. Basu PS, Ali M, Chaturvedi SK (2004) Adaptation of photosynthetic components of chickpea to water stress. In: 4th Int Crop Science Congress. Brisbane Australia, 26th Sept–10th Oct 2004Google Scholar
  8. Bates LS, Waldren RP, Teari D (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207Google Scholar
  9. Bayoumi TY, Eid MH, Metwali EM (2008) Application of physiological and biochemical indices as a screening technique for drought tolerance in wheat genotypes. Afr J Biotech 7:2341–2352Google Scholar
  10. Beauchamp F (1971) Superoxide dismutase: Improved assays and assay applicable to acrylamide gels. Anal Biochem 44:276–287Google Scholar
  11. Bhatt RM, Srinivasa Rao NK (2005) Influence of pod load response of okra to water stress. Indian J Plant Physiol 10:54–59Google Scholar
  12. Chaparzadeh N, D’Amico ML, Khavari Nejad RA, Izzo R, Navari Izzo F (2004) Antioxidative responses of Calendula officinalis under salinity conditions. Plant Physiol Biochem 42:695–701PubMedGoogle Scholar
  13. Cornic G, Massacci A (1996) Leaf photosynthesis under drought stress. In: Baker NR (ed) Advances in photosynthesis: photosynthesis and the environment, vol 5. Kluwer Academic Publishers, Dordrecht, pp 347–366Google Scholar
  14. Cortes PM, Suidaira TR (1986) Gas exchange of field grown soybean under drought. Agron J 78:454–458Google Scholar
  15. Deshmukh PS, Sairam RK, Shukla DS (1991) Measurement of ion leakage as a screening technique for drought resistance in wheat genotypes. Indian J Plant Physiol 34:89–91Google Scholar
  16. Dhindsa RH, Plumb Dhindsa R, Thorpe TA (1981) Leaf senescence correlated with increased level of membrane permeability, lipid peroxidation and decreased level of Superoxide Dismutase and Catalase. J Exp Bot 32:93–101Google Scholar
  17. Flexas J, Medrano H (2002) Drought-inhibition of photosynthesis in C-3 plants: Stomatal and non-stomatal limitation revisited. Ann Bot 89:183–189PubMedPubMedCentralGoogle Scholar
  18. Gueta-Dahan Y, Yaniv Z, Zilinskas BA, BenHayyim G (1997) Salt and oxidative stress: similar and specific responses and their relation to salt tolerance in citrus. Planta 203:460–469PubMedGoogle Scholar
  19. Hasheminasab H, Assad MT, Aliakbari A, Sahhafi R (2012) Influence of drought stress on oxidative damage and antioxidant defense systems in tolerant and susceptible wheat genotypes. J Agric Sci 4(8):20–30. Google Scholar
  20. Heath RL, Packer L (1968) Photo peroxidation in isolated chloroplast: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198PubMedGoogle Scholar
  21. Herbinger K, Tausz M, Wonisch A, Soja G, Sorger A, Grill D (2002) Complex interactive effects of drought and ozone stress on the antioxidant defence systems of two wheat cultivars. Plant Physiol Biochem 40:691–696Google Scholar
  22. Jain M, Mathur G, Koul S, Sarin NB (2001) Ameliorating effects of proline on salt stress lipid peroxidation in cell lines of groundnut (Arachis hypogea L.). Plant Cell Rep 20:463–468Google Scholar
  23. Jaleel CA, Gopi R, Sankar B, Manivannan P, Kishorekumar A, Sridharan R, Panneerselvam R (2007) Studies on germination, seedling vigour, lipid peroxidation and proline metabolism in Catharanthus roseus seedlings under salt stress. South Afr J Bot 73:190–195Google Scholar
  24. Katsuhara M, Otsuka T, Ezaki B (2005) Salt stress-induced lipid peroxidation is reduced by glutathione S-transferase but this reduction of lipid peroxides is not enough for a recovery of root growth in Arabidopsis. Plant Sci 169:369–373Google Scholar
  25. Kiani SP, Maury P, Sarrafi A, Grieu P (2008) QTL analysis of chlorophyll fluorescence parameters in sunflower (Helianthus annuus L.) under well-watered and water-stressed conditions. Plant Sci 175:565–573Google Scholar
  26. Kochert G (1978) Carbohydrate determination by phenol sulphuric acid method. In: Hellebust JA, Craigie JS (eds) Handbook of physiological methods. Cambridge University Press, Cambridge, pp 95–97Google Scholar
  27. Kpyoarissis A, Petropoulou Y, Manetas Y (1995) Summer survival of leaves in a soft-leaved shrub (Phlomis fruticose L., Labiatae) under Mediterranean field conditions: avoidance of photo-inhibitory damage through decreased chlorophyll contents. J Exp Bot 46:1825–1831Google Scholar
  28. Kumar RR, Karajol K, Naik GR (2011) Effect of polyethylene glycol induced water stress on physiological and biochemical responses in pigeon pea (Cajanus cajan L. Mill sp.). Recent Res Sci Tech 3:148–152Google Scholar
  29. Largrimini LM (1991) Wound-induced deposition of polyphenols in transgenic plants over expressing peroxidase. Plant Physiol 96(2):577–583Google Scholar
  30. Maheswari M, Vijaya Lakshmi T, Varalaxmi Y, Sarkar B, Yadav SK, Singh J, Seshu Babu G, Kumar A, Sushma A, Jyothilakshmi N, Vanaja M (2016) Functional mechanisms of drought tolerance in maize through phenotyping and genotyping under well watered and water stressed conditions. Eur J Agron 79:43–57Google Scholar
  31. Manivannan P, Jaleel CA, Sankar B, Kishorekumar A, Somasundaram R, Alagu Lakshmanan GM, Panneerselvam R (2007) Growth, biochemical modifications and proline metabolism in Helianthus annuus L. as induced by drought stress. Colloids Surf B Biointerfaces 59:141–149PubMedGoogle Scholar
  32. Marcinska I, CzyczyoMysza I, Skrzypek E, Filek M, Grzesiak S, Grzesiak MT, Janowiak F, Hura T, Dziurka M, Dziurka K, Nowakowska A, Quarrie SA (2013) Impact of osmotic stress on physiological and biochemical characteristics in drought susceptible and drought-resistant wheat genotypes. Acta Physiol Plant 35:451–461Google Scholar
  33. Mohammadkhani N, Heidari R (2008) Drought induced accumulation of soluble sugars and proline in two maize varieties. World Appl Sci J 3(3):448–453Google Scholar
  34. Mondal C, Bandopadhyay P, Alipatra A, Banerjee H (2012) Performance of summer mungbean [Vigna radiata (L.) Wilczek] under different irrigation regimes and boron levels. J Food Legumes 25:37–40Google Scholar
  35. Mwale SS, Amzad-Ali SN, Massawe FJ (2007) Growth and development of bambara groundnut in response to soil moisture: 1. Dry matter and yield. Eur J Agron 26:345–353Google Scholar
  36. Nilsen ET, Orcutt DM (1996) The physiology of plants under stress. Wiley, New York, pp 322–361Google Scholar
  37. Pireivatloum J, Qasimov N, Maralian H (2010) Effect of soil water stress on yield and proline content of four wheat lines. Afr J Biotechnol 9:36–40Google Scholar
  38. Pratap V, Sharma YK (2010) Impact of osmotic stress on seed germination and seedling growth in black gram (Phaseolus mungo). J Environ Biol 31(5):721–726PubMedGoogle Scholar
  39. Rahdari P, Hosseini SM, Tavakoli S (2012) The studying effect of drought stress on germination, proline, sugar, lipid, protein and chlorophyll content in purslane (Portulaca oleracea L.) leaves. J Med Plants Res 6:1539–1547Google Scholar
  40. Salwa AR, Hammad Osama AM, Ali (2014) Physiological and biochemical studies on drought tolerance of wheat plants by application of amino acids and yeast extract. Annals Agri Sci 59:133–145Google Scholar
  41. Shanker AK, Maheswari M, Yadav SK, Desai S, Bhanu D, Attal NB, Venkateswarlu B (2014) Drought stress responses in crops. Funct Integr Genomics 14:11–22PubMedGoogle Scholar
  42. Singh DP, Ahlawat IPS (2005) Green gram (Vigna radiata L. Wilczek) and black gram (Vigna mungo L. Hepper) improvement in India: past, present and future prospects. Indian J Agr Sci 75:243–250Google Scholar
  43. Subramanian VB, Maheswari M (1990a) Stomatal conductance, photosynthesis and transpiration in mungbean during and after relief of water stress. Indian J Exp Biol 28:542–544Google Scholar
  44. Subramanian VB, Maheswari M (1990b) Physiological responses of groundnut to water stress. Indian J Plant Physiol 33:130–135Google Scholar
  45. Tenhunen JD, Pearcy RW, Lange OL (1987) Diurnal variations in leaf conductance and gas exchange in natural environments. In: Zeiger E, Farquhar GD, Cowan IR (eds) Stomatal Function. Stanford University Press, Stanford, pp 323–351Google Scholar
  46. Uprety DC, Bhatia A (1989) Effect of water stress on the photosynthesis, productivity and water status of mungbean (Vigna radiata L. Wilczek). J Agron Crop Sci 163:115–123Google Scholar
  47. Verbruggen N, Hermans C (2008) Proline accumulation in plants: a review. Amino Acids 35:753–759PubMedGoogle Scholar
  48. Vinocur B, Altman A (2005) Recent advances in engineering plant tolerance to abiotic stress: Achievements and limitations. Curr Opin Biotechnol 16:123–132PubMedGoogle Scholar
  49. Yancy PH, Clark ME, Hand SC, Bowlus RD, Somero GN (1982) Living with water stress: evolution of osmolyte systems. Science 217:1214–1222Google Scholar
  50. Yokota A, Takahara K, Akashi K (2006) Water stress. In: Madhava Rao KV, Raghavendra AS, Janardhan Reddy K (eds) Physiology and molecular biology of stress tolerance in plants. Springer, Dordrecht, pp 15–39. Google Scholar
  51. Zlatev Z, Lidon FC (2012) An overview on drought induced changes in plant growth, water relations and photosynthesis. Emir J Food Agric 24:57–72Google Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2019

Authors and Affiliations

  1. 1.Division of Crop SciencesICAR-Central Research Institute for Dryland AgricultureSantoshnagar, HyderabadIndia
  2. 2.Indian Institute of Pulses ResearchKalyanpur, KanpurIndia

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