Acta Physiologiae Plantarum

, 40:207 | Cite as

Impact of nitric oxide on shelf life and quality of nectarine (Prunus persica var. nucipersica)

  • Smruthi Jayarajan
  • R. R. Sharma
Original Article


Nectarine is an emerging fruit crop in India which has immense nutritional quality and fairly good amount of antioxidants. In India, peach orchards are being replaced by nectarine primarily because of fuzzless peel. However, shelf life of nectarine is lean due to its climacteric behaviour. Hence, we attempted to observe the effect of nitric oxide (NO), using its donor compound, namely sodium nitroprusside (SNP) on postharvest life and quality of ‘Silver Queen’ nectarine fruit. In this study, fruit of ‘Silver Queen’ nectarine were treated with various concentrations of SNP (0.25 mM, 0.5 mM, 1.0 mM, 1.5 mM) after harvesting at climacteric stage of maturity. Fruit were stored at ambient condition for further analysis on daily basis, after air drying at room temperature. Our results revealed that among the various concentrations of SNP, 0.5 mM was found to be best in reducing physiological loss in weight (PLW), maintaining firmness and retaining higher phenolics, antioxidant activity, exhibiting slower increase in lipoxygenase (LOX) and pectin methylesterase (PME) activity and better quality fruit up to 8 days than 4 days of untreated fruit. Hence, postharvest dip of ‘Silver Queen’ nectarine fruit in 0.5 mM solution of SNP could be recommended for enhancing the shelf life by 4 days.


Antioxidant activity Lipoxygenase Nectarine Pectin methyl esterase Sodium nitroprusside 





Cupric reducing antioxidant activity




Nitric oxide




Physiological loss in weight


Pectin methyl esterase



We gratefully acknowledge Dr. Jayant Kumar, Associate Director, Regional Horticulture Research Station, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Bajaura (H.P.) for providing fruit of ‘Silver Queen’ nectarine for conducting the present study. Both the authors declare that there is no conflict of interest and approve the final article.


  1. Agar T, Polate A (1995) Effect of different packing materials on the storage quality of some apricot varieties. Acta Hortic 384:625–631CrossRefGoogle Scholar
  2. Aly MM, Agamy SZ, Biggs RH (1981) Ethylene production and firmness of peach and nectarine fruit as related to storage. Proc Fla State Hort Soc 94:291–294Google Scholar
  3. Apak R, Guclu K, Ozyurek M, Karademir SE (2004) Novel total antioxidants capacity index for dietary polyphenol and vitamins C and E using their cupric ion reducing capability in the presence of neocuprine: CUPRAC method. J Agric Food Chem 52:7970–7981CrossRefGoogle Scholar
  4. Bal JS (2006) Fruit growing. Kalyani Publishers, LudhianaGoogle Scholar
  5. Barman K. Biologically safe approaches to control sap burn, chilling injury and postharvest diseases of mango. Ph.D. Thesis. I.A.R.I., New Delhi, India, 2013Google Scholar
  6. Barman K, Siddiqui MW, Patel VB, Prasad M (2014) Nitric oxide reduces pericarp browning and preserves bioactive antioxidants in litchi. Sci Hortic 171:71–77CrossRefGoogle Scholar
  7. Colaric M, Robert V, Stampar F, Hudina M (2005) Evaluation of peach and nectarine fruit quality and correlations between sensory and chemical attributes. J Sci Food Agri 85:2611–2616CrossRefGoogle Scholar
  8. Duan XW, Su XG, You YL, Qu HX, Li YB, Jiang YM (2007) Effect of nitric oxide on pericarp browning of harvested longan fruit in relation to phenolics metabolism. Food Chem 104:571–576CrossRefGoogle Scholar
  9. Egea MI, Martinez-Madrid MC, Sanchez-Bel P, Muricia Romojaro F (2007) The influence of electron-beam ionization on ethylene metabolism and quality parameter in apricot (Prunus armeniaca L., cv. Builda), Swiss Soc. Food Sci Technol 40:1027–1035Google Scholar
  10. Flores F, Sanchez-Bel P, Valdenegro M, Romojaro F, Martinez-Madrid M, Egea M (2008) Effects of a pretreatment with nitric oxide on peach (Prunus persica L.) storage at room temperature, Eur. Food Res Technol 227:1599–1611CrossRefGoogle Scholar
  11. Gil MI, Tomas F, Betty BA, Pierce H, Kader AA (2002) Antioxidant capacities, phenolic compounds, carotenoids, and vitamin C contents of nectarine, peach, and plum cultivars from California. J Agric Food Chem 50:4976–4982CrossRefGoogle Scholar
  12. Hagerman AE, Austin PJ (1986) Continuous spectrophotometry assay for plant pectin methyl esterase. J Agri Food Chem 34:440–444CrossRefGoogle Scholar
  13. Jhalegar Md J, Sharma RR, Pal RK, Rana V (2012) Effect of postharvest treatments with polyamines on physiological and biochemical attributes of kiwifruit (Actinidia deliciosa). CV Allison Fruit 67:13–22CrossRefGoogle Scholar
  14. Kader AA, Kasmire RF, Mitchell EG, Reid MS, Sommer NF, Thompson JE (2002) Postharvest technology of horticultural crops. University of California, Division of Agriculture and Natural Resources, OaklandGoogle Scholar
  15. Ku VVV, Wills RBH, Leshem YY (2000) Use of nitric oxide to reduce postharvest water loss from horticultural produce. J Hort Sci Biotechnol 75:268–270CrossRefGoogle Scholar
  16. Manjunatha G, Lokesh V, Neelwarne B (2010) Nitric oxide in fruit ripening: trends and opportunities, Biotechnol. Adv 28:489–499Google Scholar
  17. Panse VG, Sukhatme PV (1984) Statistical methods for agricultural workers, 3rd edn. Indian Council of Agricultural Research, New DelhiGoogle Scholar
  18. Perez AG, Sanz C, Olias R, Olias JM (1999) Lipoxygenase and hydroperoxidelyase activities in ripening strawberry fruit. J Agric Food Chem 47:249–253CrossRefGoogle Scholar
  19. Ranganna S (1999) Handbook of analysis and quality control for fruit and vegetable products, 2nd edn. Tata McGraw-Hill Publishing Company Ltd, New DelhiGoogle Scholar
  20. Sharma RR, Krishna H (2016) Fruit production: minor fruit. Daya Publishing House, DelhiGoogle Scholar
  21. Sharma S, Sharma RR (2015) Nitric oxide inhibits activities of PAL and PME enzymes and reduces chilling injury in ‘Santa Rosa’ Japanese plum (Prunus salicina Lindell). J Plant Biochem Biotechnol 24:292–297CrossRefGoogle Scholar
  22. Sharma S, Sharma RR, Pal RK, Jhalegar MJ, Singh J, Srivastav M, Dhiman MR (2011) Ethylene absorbents influence fruit firmness and activity of enzymes involved in fruit softening of Japanese plum (Prunus salicina Lindell) cv. Santa Rosa. Fruit 67:257–266CrossRefGoogle Scholar
  23. Sharma RR, Jhalegar MJ, Jha SK, Rana V (2015) Genotypic variation in total phenolics, antioxidant activity, enzymatic activity and quality attributes among kiwifruit cultivars. J Plant Biochem Biotechnol 24:114–119CrossRefGoogle Scholar
  24. Singh SP, Swinny EE (2009) Post harvest nitric oxide fumigation delays fruit ripening and alleviates chilling injury during cold storage of Japanese plums (Prunus salicina L), Postharvest Biol. Technol 53:101–108Google Scholar
  25. Singh Z, Khan AS, Zhu S, Payne AD (2013) Nitric oxide in the regulation of fruit ripening: challenges and thrusts. Stewart Postharvest Rev 9:1–11Google Scholar
  26. Singleton VL, Orthofer R, Lamuela- Raventos RM (1999) Analysis of total phenols, other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 29:152–178CrossRefGoogle Scholar
  27. Wen IC, Koch KE, Sherman WB (1995) Comparing fruit and tree characteristics of two peaches and their nectarine mutants. J Am Soc Hort Sci 120:101–106Google Scholar
  28. Wendehenne D, Durner J, Klessig DF (2004) Nitric oxide: a new player in plant signaling and defense responses. Curr Opin Plant Biol 7:449–455CrossRefGoogle Scholar
  29. Wrolstad RE, Durst RW, Lee J (2005) Tracking color and pigment changes in anthocyanin products. Trends Food Sci Technol 16:423–428CrossRefGoogle Scholar
  30. Zhang DD, Cheng GP, Li J, Yi C, Yang E, Qu HX, Jiang YM, Duan XW (2008) Effect of nitric oxide on disorder development and quality maintenance of plum fruit stored at low temperature. Acta Hortic 804:549–554CrossRefGoogle Scholar
  31. Zhu SH, Zhou J (2007) Effect of nitric oxide on ethylene production in strawberry fruit during storage. Food Chem 100:1517–1522CrossRefGoogle Scholar
  32. Zhu S, Lina S, Mengchen L, Jie Z (2008) Effect of nitric oxide on reactive oxygen species and antioxidant enzymes in kiwifruit during storage. J Sci Food Agric 88:2324–2331CrossRefGoogle Scholar
  33. Zhu S, Sun L, Zhou J (2009) Effects of nitric oxide fumigation on phenolic metabolism of postharvest Chinese winter jujube (Zizyphus jujuba Mill. cv. Dongzao) in relation to fruit quality. LWT-Food Sci Technol 42:1009–1014CrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2018

Authors and Affiliations

  1. 1.Division of Food Science and Postharvest TechnologyICAR-IARINew DelhiIndia

Personalised recommendations