Stomatal responses to long-term high vapor pressure deficits mediated most limitation of photosynthesis in tomatoes
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Plants grown at high vapor pressure deficit (VPD) usually present decreased photosynthesis, but stomatal and mesophyll limitation to photosynthesis remain poorly quantified. To better understand the regulation of high VPD on photosynthesis and plant growth in tomatoes, we investigated the limitation of stomatal conductance and mesophyll conductance to photosynthesis and relative importance of stomatal morphology and function in stomatal conductance. Both the net photosynthesis rate and total biomass were significantly limited by high VPD. Meanwhile, stomatal conductance and mesophyll conductance were decreased under high VPD. The stomatal conductance limitation was responsible for 60% of the total photosynthetic limitation. Moreover, a reduction in stomatal density and stomatal size occurred under high VPD, which was significantly correlated with the down-regulation of stomatal conductance. The stomatal morphology contributed to more than half the change in stomatal conductance. Nevertheless, stomatal movement was also an important factor in regulating stomatal conductance. The decrease of hydraulic conductance and transpiration rate with no significant difference in relative water content, leaf water potential, and/or osmotic potential suggested passive hydraulic regulation in the feedforward responses of stomata to high VPD.
KeywordsPhotosynthesis Stomatal conductance Stomatal morphology Stomatal behavior Tomato Vapor pressure deficit
This work was supported by the National Science Foundation of China .
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Conflict of interest
The authors declare no conflicts of interest.
- Galle A, Florez-Sarasa I, Tomas M, Pou A, Medrano H, Ribas-Carbo M, Flexas J (2009) The role of mesophyll conductance during water stress and recovery in tobacco (Nicotiana sylvestris): acclimation or limitation? J Exp Bot 60:2379–2390. https://doi.org/10.1093/jxb/erp071 CrossRefPubMedGoogle Scholar
- Galmés J, Ochogavía JM, Gago J, Roldán EJ, Cifre J, Conesa M (2013) Leaf responses to drought stress in Mediterranean accessions of Solanum lycopersicum: anatomical adaptations in relation to gas exchange parameters. Plant Cell Environ 36:920–935. https://doi.org/10.1111/pce.12022 CrossRefPubMedGoogle Scholar
- Laisk AK (1977) Kinetics of photosynthesis and photorespiration in C3-plants. Nauka, MoscowGoogle Scholar
- Liu YY, Song J, Wang M, Li N, Niu CY, Hao GY (2015b) Coordination of xylem hydraulics and stomatal regulation in keeping the integrity of xylem water transport in shoots of two compound-leaved tree species. Tree Physiol 35:1333–1342. https://doi.org/10.1093/treephys/tpv061 CrossRefPubMedGoogle Scholar
- Martins SCV, McAdam SAM, Deans RM, DaMatta FM, Brodribb TJ (2016) Stomatal dynamics are limited by leaf hydraulics in ferns and conifers: results from simultaneous measurements of liquid and vapour fluxes in leaves. Plant Cell Environ 39:694–705. https://doi.org/10.1111/pce.12668 CrossRefPubMedGoogle Scholar
- Perez-Martin A, Flexas J, Ribas-Carbó M, Bota J, Tomás M, Infante JM, Diaz-Espejo A (2009) Interactive effects of soil water deficit and air vapour pressure deficit on mesophyll conductance to CO2 in Vitis vinifera and Olea europaea. J Exp Bot 60:2391–2405. https://doi.org/10.1093/jxb/erp145 CrossRefPubMedGoogle Scholar
- Qiu C, Ethier G, Pepin S, Dubé P, Desjardins Y, Gosselin A (2017) Persistent negative temperature response of mesophyll conductance in red raspberry (Rubus idaeus L.) leaves under both high and low vapour pressure deficits: a role for abscisic acid? Plant. Cell Environ 40:1940–1959. https://doi.org/10.1111/pce.12997 CrossRefGoogle Scholar
- Rodriguez-Dominguez CM, Buckley TN, Egea G, de Cires A, Hernandez-Santana V, Martorell S, Diaz-Espejo A (2016) Most stomatal closure in woody species under moderate drought can be explained by stomatal responses to leaf turgor. Plant Cell Environ 39:2014–2026. https://doi.org/10.1111/pce.12774 CrossRefPubMedGoogle Scholar
- Zhang DL, Zhang ZD, Li JM, Chang YB, Du QJ, Pan TH (2015) Regulation of vapor pressure deficit by greenhouse micro-fog systems improved growth and productivity of tomato via enhancing photosynthesis during summer season. PloS One 10:e0133919. https://doi.org/10.1371/journal.pone.0133919 CrossRefPubMedPubMedCentralGoogle Scholar