A comparative cytogenetic study of 17 Avena species using Am1 and (GAA)6 oligonucleotide FISH probes
- 19 Downloads
Fluorescence in situ hybridization (FISH) was performed to explore the genomic and species relationships among 17 taxa using Am1 (oligo-Am1) and (GAA)6 oligonucleotide probes. Oligo-Am1 (51 bp) hybridized strongly over almost the entire length of all chromosomes in the C genome. Six translocations between the A and C genomes were found in AACC tetraploids and AACCDD hexaploids, four minor translocations between the C and D genomes were found in AACCDD hexaploids, and two large translocations between the C and D genomes were found in A. sativa. In the 17 Avena species, (GAA)6 regions mainly appear as sharp, thin bands at pericentromeric positions in the A, B, and C genomes and at termini in the B genome. However, no (GAA)6 signal loci were observed in the D genome. The (GAA)6 signal number was constant in both AA and CC diploids, though with different signal intensities. The (GAA)6 signal pattern was diverse in AABB, AACC, and AACCDD polyploids, with each species exhibiting one signal pattern. The (GAA)6 signal number was consistent in diploids and varied in polyploids, describing an intragenomic relationship among Avena species. This study is the first to test these two oligonucleotides, which are based on synthesized repeat units (18–51 bp), in the genus Avena. Our approach paves the way for future studies in which FISH probes can be used to assign other landmark genomic sequence oligonucleotides to physical chromosomes.
KeywordsFISH GAA Oligonucleotides Pericentromeric band
This study was supported by the Natural Science Foundation of China (31500993). The authors greatly appreciate the American National Plant Germplasm System (Pullman, WA, USA) and Plant Gene Resources of Canada (Saskatoon, SK, Canada) for providing material for this investigation.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
- Baum BR (1977) Oats: wild and cultivated. In: A monograph of the genus Avena L. (Poaceae). Biosystematics Research Institute, Department of Agriculture, Research Branch. Ottawa, CanadaGoogle Scholar
- Fominaya A, Loarce Y, Montes A, Ferrer E (2017) Chromosomal distribution patterns of the (AC)10 microsatellite and other repetitive sequences, and their use in chromosome rearrangement analysis of species of the genus Avena. Genome 60(3):216–227. https://doi.org/10.1139/gen-2016-0146 CrossRefPubMedGoogle Scholar
- Jellen EN (2016) C-banding of plant chromosomes. In: Kianian SF, Kianian PMA (eds) Plant cytogenetics. Springer, New York, pp 1–5Google Scholar
- Leggett JM (1996) Using and conserving Avena genetic resources. In: Scoles GJ, Rossnagel BG (eds) Proceedings of the 5th international oat conference and 7th international barley genetic. Symposium. University of Saskatchewan, Saskatoon, pp 128–132Google Scholar
- Luo X, Tinker NA, Zhang H, Wight CP, Kang H, Fan X, Wang Y, Sha L, Zhou Y (2015) Centromeric position and genomic allocation of a repetitive sequence isolated from chromosome 18D of hexaploid oat, Avena sativa L. Genet Resour Crop Evol 62(1):1–4. https://doi.org/10.1007/s10722-014-0170-x CrossRefGoogle Scholar
- Luo X, Tinker NA, Zhou Y, Liu J, Wan W, Chen L (2018b) Chromosomal distributions of oligo-Am 1 and (TTG)6 trinucleotide and their utilization in genome association analysis of sixteen Avena species. Genetic Resources & Crop Evolution 2018:1–11. https://doi.org/10.1007/s10722-018-0639-0 Google Scholar
- Moyzis RK, Buckingham JM, Cram LS, Dani M, Deaven LL, Jones MD, Meyne J, Ratliff RL, Wu JR (1988) A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes. Proc Natl Acad Sci 85(18):6622–6626. https://doi.org/10.1073/pnas.85.18.6622 CrossRefPubMedGoogle Scholar
- Rajhathy T, Thomas H (1974) Cytogenetics of oats (Avena L.). Genetics Society of Canada, OttawaGoogle Scholar
- Röder MS, Lapitan NL, Sorrells ME, Tanksley SD (1993) Genetic and physical mapping of barley telomeres.[J]. Molecular & General Genetics 238(1–2):294–303 PMID: 8479435Google Scholar
- Rodionov AV, Tyupa NB, Kim ES, Machs EM, Loskutov IG (2005) Genomic configuration of the autotetraploid oat Species Avena macrostachya inferred from comparative analysis of ITS1 and ITS2 sequences: on the oat karyotype evolution during the early events of the Avena species divergence. Russ J Genet 41(5):518–528. https://doi.org/10.1007/s11177-005-0120-y CrossRefGoogle Scholar
- Thomas H (1989) New species of Avena. In: Proceedings of the 3rd international oat conference, Lund, pp 18–23Google Scholar
- Yan H, Bekele WA, Wight CP, Peng Y, Langdon T, Latta RG, Fu YB, Diederichsen A, Howarth CJ, Jellen EN, Boyle B, Wei Y, Tinker NA (2016) High-density marker profiling confirms ancestral genomes of Avena species and identifies D-genome chromosomes of hexaploid oat. Theor Appl Genet 129(11):2133–2149. https://doi.org/10.1007/s00122-016-2762-7 CrossRefPubMedPubMedCentralGoogle Scholar