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Genetic mapping and salt tolerance of a novel D1-allelic mutant of rice (Oryza sativa L.)

  • Wei-jiang Tian
  • Xiao-qiong Zhang
  • Xiao-wen Wang
  • Jia Xie
  • Yang-yang Li
  • Ying Sun
  • Yi-ran Tao
  • Yu-zhen Xiong
  • Xian-chun Sang
Original Article

Abstract

Plant height and grain shape are important traits that may affect yield in rice, and they therefore have enormous importance in breeding. A dwarf small-grain mutant (S525) was identified among progeny of the Indica rice restorer line ‘Xida 1B’ (wild type) raised from seeds treated with ethyl methanesulfonate. The dwarf and small-grain phenotypes were stably inherited after multi-generation selfing. Field-grown mutant plants showed the phenotypes of dwarfism, broad leaves, and small round grains. Genetic mapping and sequencing confirmed that S525 was a novel d1-allelic mutant. A single-base transition (G to A) in the functional dwarfism gene D1 at the conjunction site of the 11th intron caused excision or duplication of the 11th exon in the mRNA and resulted in translation of a defective Gα protein. The S525 showed enhanced salt tolerance compared with the wild type (WT), and the expression of genes associated with salt tolerance quantitatively increased in response to treatment with 200 mM NaCl. The S525 may be useful for future investigation of Gα functions and in the breeding of new dwarf rice cultivars.

Keywords

Rice D1 Gene mapping Salt tolerance 

Notes

Acknowledgements

This work was supported by Chongqing Science and Technology Key Project (cstc2017shms-xdny80057) and the Fundamental Research Funds for the Central Universities (XDJK2015C118).

Author contribution statement

The contribution of each author to the manuscript: XS and WT planned and designed the research. WT, XZ, XW, and JX performed experiments. XS and WT conducted fieldwork. YL, YS, YT, and YX analyzed data. XS, WT, and XZ wrote the manuscript.

Supplementary material

11738_2018_2717_MOESM1_ESM.docx (25 kb)
Supplementary material 1 (DOCX 24 KB)

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2018

Authors and Affiliations

  1. 1.Key Laboratory of Application and Safety Control of Genetically Modified Crops, College of Agronomy and BiotechnologySouthwest UniversityChongqingChina

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