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Identification and transcriptional analysis of dehydrin gene family in cucumber (Cucumis sativus)

  • Yong Zhou
  • Lifang Hu
  • Shuiyan Xu
  • Lunwei Jiang
  • Shiqiang Liu
Original Article
  • 98 Downloads

Abstract

Dehydrins (DHNs) are a group II late embryogenesis abundant (LEA) proteins that play essential roles in plant growth, development and responses to diverse environmental stimuli. Here, four DHNs in cucumber genome were identified using bioinformatics-based methods according to the highly conserved K-, Y- and S-segments, including 1 YnKn-type, 2 YnSKn-type, and 1 SKn-type DHNs. All of them are intrinsically disordered proteins (IDPs) and possess a large number of disorder-promoting amino acids. Secondary structure prediction revealed that each of them is composed of high proportion of alpha helix and random coil. Gene structure and phylogenetic analyses with DHNs from cucumber and several other species revealed that some closely related DHN genes had similar gene structures. A number of cis-elements involved in stress responses and phytohormones were found in each CsDHN promoter. The tissue expression profiles suggested that the CsDHN genes have overlapping, but different expression patterns. qRT-PCR results showed that three selected CsDHN genes could respond to heat, cold, osmotic and salt stresses, as well as to signaling molecules such as H2O2 and ABA. These results lay a solid foundation for future functional investigation of the cucumber dehydrin gene family in tissue development and stress responses in plants.

Keywords

Cucumber Dehydrin (DHN) Gene family Intrinsically disordered protein (IDP) Expression pattern Abiotic stress 

Notes

Acknowledgements

This work was financially supported by the Key Project of Youth Science Foundation of Jiangxi Province (20171ACB21025), the National Natural Science Foundation of China (31460522 and 31660578).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing financial interests.

Supplementary material

11738_2018_2715_MOESM1_ESM.doc (43 kb)
Supplementary material 1 (DOC 43 KB)
11738_2018_2715_MOESM2_ESM.doc (84 kb)
Supplementary material 2 (DOC 84 KB)
11738_2018_2715_MOESM3_ESM.xls (34 kb)
Supplementary material 3 (XLS 33 KB)

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2018

Authors and Affiliations

  1. 1.College of ScienceJiangxi Agricultural University, Nanchang Economic and Technological Development DistrictNanchangChina
  2. 2.Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, College of AgronomyJiangxi Agricultural UniversityNanchangChina

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