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Expression regulation of a mature intronic miR3029 by 5′ UTR-like

  • Qingwei Wang
  • Fosheng Li
  • Shuhua Zhu
  • Shenghua Wang
  • Wenguo Wang
  • Yang He
Original Article
  • 67 Downloads

Abstract

5′ UTR of the coding gene affected gene expression at the transcription and translation levels. For miRNA, 5′ UTR (termed 5′ UTR-like) is similar to UTR of encoding protein gene. However, the function of 5′ UTR-like has not been reported in the regulation of pre-miRNA formation and the process of miRNA’s formation. In this study, the regulated elements of 5′ UTR-like of intron miR3029 were analyzed by bioinformatics. The function analysis of primary structure of 5′ UTR-like was studied by the transient expression system of Arabidopsis thaliana protoplast. The function of second-structure stem loops of 5′ UTR-like was conducted by detecting the expression of miRNA3029 and pri-miRNA3029 in transgenic tobacco. In this study, we found that 5′ UTR-like region of miR3029 existences low temperature (LTR), oxygen stress element (ARE) and stem loop structure. These elements and structure may control miRNA’s stability and thereby affect the formation of mature miR3029. There also existed a translation enhancer similar to that of the UTRs of coding genes. The function analysis of second structure found that the predicted stem loop 5 can promote the expression of mature miR3029; stem loops 1, 2, 3 and 4 can inhibit the formation of mature miR3029. To sum up, this study lays a foundation for exploring the mechanism of how the primary transcript is cleaved into pre-miRNA, and provides reference for the study of the regulation of miRNA.

Keywords

5′ UTR-like Intronic miRNA Biogenesis Rice 

Abbreviations

CBC

Nuclear cap-binding complex

SE

The C2H2 zinc-finger protein SERRATE

HYL1

The dsRNA-binding protein HYPONASTIC LEAVES1

UTR

Untranslational region

DCL1

DICER-LIKE1

LUC

Luciferase gene

pre-miRNA

Precursor miRNA

pri-miRNA

The primary of miRNA

ORF

The open reading frame

IRES

Internal ribosome entry site

Notes

Acknowledgements

This research was supported by the National Science Fund of China (Nos. 31070276 and 31270360) and Genetically Modified Organisms Breeding Major Projects (2016ZX08010001-10).

Compliance with ethical standards

Conflict of interest

All of the authors declare that they have no competing interests.

Supplementary material

11738_2018_2713_MOESM1_ESM.doc (97 kb)
Supplementary material 1 (DOC 97 KB)
11738_2018_2713_MOESM2_ESM.doc (40 kb)
Supplementary material 2 (DOC 40 KB)

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2018

Authors and Affiliations

  1. 1.The Sichuan Provincial Key Laboratory for Human Disease Gene StudyHospital of the University of Electronic Science and Technology of China and Sichuan Provincial People’s HospitalChengduChina
  2. 2.School of Medical TechnologyChengdu University of Traditional Chinese MedicineChengduChina
  3. 3.National and Local Joint Engineering Laboratory for Energy Plant Bio-oil Production and Application, College of Life ScienceSichuan UniversityChengduChina
  4. 4.Biogas Institute of Ministry of AgricultureChengduChina

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