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Manipulation of cellular energy reveals the relationship between ultraweak luminescence and cellular energy during senescence of strawberry (Fragaria × ananassa) fruits

  • Jinli Guo
  • Huan Liu
  • Yang Bai
  • Yutong Yan
  • Lianguo Li
Original Article
  • 43 Downloads

Abstract

The goal of this study was to investigate the ultraweak luminescence (UWL) of strawberry fruits in relation to mitochondrial functions and energy production during strawberry senescence. Fully ripe strawberry fruits and mitochondria isolated from those fruits were treated with either adenosine triphosphate (ATP) or the respiratory chain uncoupler 2,4-dinitrophenol (DNP). The activities of H+-ATPase and Ca2+-ATPase, the content of ATP, the free radical O2 as well as the UWL intensity were measured. Our results showed that activities of H+-ATPase and Ca2+-ATPase as well as the ATP content gradually decreased during fruit senescence in all three groups. Compared with the control, DNP treatment exacerbated, while ATP treatment reduced the decrease of H+-ATPase and Ca2+-ATPase activity, the energy charge and UWL intensity. UWL intensity was positively correlated with mitochondrial function and ATP content. Our results strongly suggest that mitochondria are a major source of UWL of strawberry fruits, and that the cellular energy ATP plays important roles in senescence of strawberry fruits, and in UWL formation. Our study provides convincing evidence of the interrelationship between cellular energy and UWL, which helps researchers to better understand the process of senescence in strawberry fruits.

Keywords

Cellular energy Mitochondria Strawberry fruit senescence Ultraweak luminescence 

Notes

Acknowledgements

This work was financially supported by the Natural Science Foundation of China (Grant No. 31260455).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Human and animal rights statement

This study did not involve human participants and/or animals.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2018

Authors and Affiliations

  • Jinli Guo
    • 1
  • Huan Liu
    • 1
  • Yang Bai
    • 1
  • Yutong Yan
    • 1
  • Lianguo Li
    • 1
  1. 1.College of AgronomyInner Mongolia Agricultural UniversityHohhotChina

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