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The rhizosphere microbial community response to a bio-organic fertilizer: finding the mechanisms behind the suppression of watermelon Fusarium wilt disease

  • Jia Zhao
  • Yuguo WangEmail author
  • Hong Liang
  • Jing Huang
  • Zhe Chen
  • Yuanjun Nie
Original Article

Abstract

We aimed to evaluate the capability of bio-organic fertilizer suppressing watermelon Fusarium wilt disease, compare the variations of the rhizosphere bacterial and fungal community compositions after treatment with different fertilizers, and explore mechanisms causing disease suppression in rhizosphere microbial community. A rhizobacterium (Bacillus amyloliquefaciens JDF35) was identified to control watermelon Fusarium wilt disease. Bio-organic fertilizer JDF35 (BOF) was generated by inoculating JDF35 into the organic fertilizer (OF) composed of cow and chicken manure compost (1:50 v/w). A three successive growing season pot experiment was designed to evaluate the effects of BOF compared with OF and chemical fertilizer (CF). Next-generation sequencing using the Illumina MiSeq platform was used to investigate the variations in rhizosphere microbial community composition. The growth of the watermelon plants, soil pH, and available N, P and K concentrations were the highest in the BOF treatment. The Fusarium wilt incidence in the BOF treatment was lower than that in the CF and OF treatment, and the differences for disease incidence were significant (P < 0.001). The diversity of the rhizosphere bacterial community was higher, and that of the fungal was lower in the BOF treatment. Most importantly, the BOF treatment had lowest abundances of Fusarium. The application of the BOF altered the composition of rhizosphere microbial community, suppressing Fusarium wilt disease and promoting plant growth.

Keywords

Watermelon Bacillus amyloliquefaciens Illumina MiSeq platform Fusarium wilt disease suppression Bio-organic fertilizer 

Notes

Acknowledgements

We thank Institute of agro-products quality safety and testing research for generously providing FON.

Compliance with ethical standards

Funding

This work was financially supported in part by grants from the Independent Innovation of Science and Technology Program of Shanxi Province (2015zzcx-22), Shanxi Academy of Agricultural Science Scientific and Technological Research Projects (YGG1414), and the Youth's Foundational Research Project of Shanxi Province (201701D221169).

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11738_2017_2581_MOESM1_ESM.tiff (7 mb)
Fig. S1 Phylogenetic tree (Neighbor-Joining Tree), based on 16S rRNA gene sequences, showing the relationship between Bacillus amyloliquefaciens strain JDF35 and other closely related strains. (TIFF 7151 kb)
11738_2017_2581_MOESM2_ESM.tif (2.4 mb)
Fig. S2 Rarefaction curves of bacteria (a) and fungi (b) at 97% similarity levels of the rhizosphere soil of each replicate (treated with bio-organic fertilizer (BOF), or cow and chicken manure compost (OF) or chemical fertilizer (CF)). (TIFF 2478 kb)
11738_2017_2581_MOESM3_ESM.docx (14 kb)
Supplementary material 3 (DOCX 13 kb)
11738_2017_2581_MOESM4_ESM.docx (14 kb)
Supplementary material 4 (DOCX 14 kb)

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2017

Authors and Affiliations

  • Jia Zhao
    • 1
    • 2
  • Yuguo Wang
    • 1
    • 2
    Email author
  • Hong Liang
    • 2
  • Jing Huang
    • 2
  • Zhe Chen
    • 2
  • Yuanjun Nie
    • 2
  1. 1.College of AgricultureShanxi Agriculture UniversityTaiguChina
  2. 2.Biotechnology Research CenterShanxi Academy of Agricultural SciencesTaiyuanChina

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