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Acta Physiologiae Plantarum

, 39:200 | Cite as

Proteomic changes in response to low-light stress during cotton fiber elongation

  • Wei Hu
  • Mi Zheng
  • Shanshan Wang
  • Yali Meng
  • Youhua Wang
  • Binglin Chen
  • John L. Snider
  • Zhiguo ZhouEmail author
Original Article

Abstract

Numerous studies have illustrated that low light is one of the major abiotic stresses limiting cotton (Gossypium hirsutum L.) fiber length, but studies addressing molecular mechanisms contributing to reduced fiber growth under low light are lacking. To investigate the molecular mechanisms of cotton fiber elongation in response to low light, an experiment of low light caused by shading was conducted with cotton cultivar NuCOTN 33B. The results showed that low light resulted in shorter fiber length. Proteomic analysis of four developmental stages (5, 10, 15 and 20 days post-anthesis) showed that 49 proteins were significantly responsive to low light. 39 differentially expressed proteins that included some known as well as some novel low-light stress-responsive proteins were identified. These differentially expressed proteins were involved in signal transduction, carbohydrate/energy metabolism, cell wall component synthesis, protein metabolism, cytoskeleton, nitrogen metabolism and stress responses. The results also showed that the decrease in fiber length might be because the levels of signal-related protein (phospholipase D), cytoskeletal proteins (two annexins isoforms), cell wall component-related proteins (sucrose synthase, UDP-d-glucuronic acid 4-epimerase and rhamnose synthase), carbohydrate metabolism-proteins (phosphofructokinase, dihydrolipoamide dehydrogenase, vacuolar H+-ATPase catalytic subunit, malate dehydrogenase and isocitrate dehydrogenase), and stress-related proteins (peroxisomal catalase, short chain alcohol dehydrogenase) were decreased under low light.

Keywords

Cotton (Gossypium hirsutum L.) Low light Fiber length Proteomics 

Abbreviations

CRLR

Crop relative light rate

FB

Fruiting branch

DPA

Days post-anthesis

PAR

Photosynthetic active radiation

2-DE

Two-dimensional electrophoresis

MDT

Mean daily temperature

MDTmax

Mean daily maximum temperature

MDTmin

Mean daily minimum temperature

LRP

Low-light-responsive protein

CBL

Calcineurin B-like protein

PLD

Phospholipase D

CAP

Cyclase binding protein

Notes

Acknowledgements

We are grateful for financial support from the National Natural Science Foundation of China (31571606), the Special Fund for Agro-scientific Research in the Public Interest (201303002), Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP) and China Agriculture Research System (CARS-18-20).

Supplementary material

11738_2017_2499_MOESM1_ESM.docx (1.3 mb)
Supplementary material 1 (DOCX 1380 kb)

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2017

Authors and Affiliations

  • Wei Hu
    • 1
  • Mi Zheng
    • 1
    • 2
  • Shanshan Wang
    • 1
  • Yali Meng
    • 1
  • Youhua Wang
    • 1
  • Binglin Chen
    • 1
  • John L. Snider
    • 3
  • Zhiguo Zhou
    • 1
    Email author
  1. 1.College of AgricultureNanjing Agricultural UniversityNanjingPeople’s Republic of China
  2. 2.State Key Laboratory of Tree Genetics and BreedingNortheast Forestry UniversityHarbinPeople’s Republic of China
  3. 3.Department of Crop and Soil SciencesUniversity of GeorgiaTiftonUSA

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