Acaricidal active fractions from acetone extract of Aloe vera L. against Tetranychus cinnabarinus and Panonychus citri
- 170 Downloads
This study aimed to isolate acaricidal active fractions from acetone extract of Aloe vera L. and investigate the toxicity of these fractions against Tetranychus cinnabarinus (T. cinnabarinus) and Panonychus citri (P. citri). Acetone extract of A. vera L. was isolated by immersing in acetone for 72 h, and diverse fractions were fractionated by column chromatography. The acaricidal activity of each fractions was evaluated by corrected mortality of T. cinnabarinus through slide-dip bioassay. The 8th and 13th fractions of acetone extract with good acaricidal activity were indentified by LC/MS, and the toxicity of these two fractions to T. cinnabarinus and P. citri was identified by regression analysis. Acetone extract of A. vera L. exhibited obvious acaricidal activity, from which a total of 18 fractions were isolated. The 8th and 13th fractions with strong acaricidal activity against T. cinnabarinus were identified to be 3-O-alpha-d-mannopyranosyl-d-mannopyranose (OAMM) and aloe emodin. When compared with spirodiclofen, both OAMM and aloe emodin exhibited higher toxicity to T. cinnabarinus, while only OAMM exhibited a higher toxicity to P. citri (P < 0.05). OAMM and aloe emodin isolated from acetone extract of A. vera L. exhibited obvious acaricidal activities against T. cinnabarinus and P. citri.
KeywordsAcetone extract A. vera L. Acaricidal activity Mites
This work was supported by the Natural Science Fund of China (31272058). We are very grateful to Meng Li, Lijuan Ding and Kai Zhang for their assistance in this work. And we thank anonymous reviewers for their valuable comments and suggestions for our manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
- Arunkumar S, Muthuselvam M (2009) Analysis of phytochemical constituents and antimicrobial activities of Aloe vera L. against clinical pathogens. World J Agric Sci 5:207–221Google Scholar
- Daizyr B, Harminderpal S, Ravinderkumar K, Shalinder K (2009) Eucalyptus essential oil as a natural pesticide. For Ecol Manag 256:2166–2174Google Scholar
- Elkhayat EF, Rady GH, Abdelzahar TR, Omar MO, Kalmosh FS (2014) Repellency and toxicity effect of some leaf extracts of Aloe vera L. against adult females of Tetranychus urticae Koch (Acari: Tetranychidae). Glob J Environ Sci Toxicol 1:145–155Google Scholar
- Omotoso OT (2008) Insecticidal and insect productivity reduction capacities of aloe vera and Bryophyllum pinnatum on Tribolium castaneum (herbst). Afr J Appl Zool Environ Biol 7:95–100Google Scholar
- Rahman MS, Ali MY, Ali MU (2009) In vitro screening of flavonoid compounds isolated from Cassia alata L. Leaves for fungicidal activities. J Bio Sci 16:139–142Google Scholar
- Senegačnik J (2000) Resistance of varroa mites against chemical acaricides. Sodob Kmet 33:111–116Google Scholar
- Zhang Q, Ding LJ, Li M, Cui WW, Wei D, Luo JX, Zhang YQ (2013) Action modes of Aloe vera L. extracts against Tetranychus cinnabarinus Boisduval (Acarina: Tetranychidae). Agric Sci 4:117–122Google Scholar