Acta Physiologiae Plantarum

, 39:63 | Cite as

Glyphosate affects chlorophyll, photosynthesis and water use of four Intacta RR2 soybean cultivars

  • Fábio Henrique KrenchinskiEmail author
  • Leandro Paiola Albrecht
  • Alfredo Junior Paiola Albrecht
  • Victor José Salomão Cesco
  • Danilo Morilha Rodrigues
  • Roberto Luis Portz
  • Luiz Henrique Saes Zobiole
Original Article


The planting of RR2 Intacta soybeans by farmers has been expanding strongly. However, some visual injuries have been noted after glyphosate application. The aim of this study was to evaluate the influence of glyphosate application on chlorophyll, photosynthesis and water use of four Intacta RR2 soybean cultivars. The experiment was conducted in a greenhouse, in a randomized block design with a 3 × 4 factorial scheme, consisting of three glyphosate rates and four soybean cultivars. The glyphosate formula used was isopropylamine salt + potassium salt. The parameters analyzed were phytotoxicity at 7, 14, 21 and 28 days after application, and total chlorophyll index at 0, 3, 7, 14, 21, 28, 35, 42 and 49 days after application. Furthermore, 40 days after application, the net CO2 assimilation rate (A), transpiration rate (E), stomatal conductance (G), and internal CO2 concentration (Ci) were evaluated as well. The water-use efficiency (WUE) and carboxylation efficiency were calculated. The data were submitted to analysis of variance and compared by the Tukey’s test (p ≤ 0.05), followed by regression analysis. The phytotoxicity influence could be seen until 21 days after application, in which Monsoy 6210 IPRO cultivar was the most injured. The increasing doses promoted a reduction of the chlorophyll level up to 35 days after application in Monsoy 6410 IPRO. The cultivars tested here showed similar chlorophyll index values. On the 3rd, 7th and 14th DAA (Fig. 5a–c), there was a significant linear decline in the chlorophyll index with rising glyphosate dose for all four cultivars. The chlorophyll index cultivars were not influenced by the doses on the 42nd and 49th DAA. There was no difference in water use and carboxylation efficiency. The parameters A, E and A/Ci showed a positive correlation as the doses increased, while Ci declined, in both cultivars. The application of glyphosate on these soybean cultivars causes different injuries according to the sensitivity. In general, RR2 soybeans have the ability to recover from visual intoxication injuries and reestablish the normal chlorophyll production and photosynthetic parameters after glyphosate application.


Roundup ready 2 soybeans Chlorophyll index CO2 assimilation rate 



Days after application


Net CO2 assimilation rate


Transpiration rate


Stomatal conductance


Internal CO2 concentration


Water-use efficiency


Carboxylation efficiency


Aminomethylphosphonic acid


Aminolaevulinic acid



Funding was provided by Universidade Federal do Parana.


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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2017

Authors and Affiliations

  • Fábio Henrique Krenchinski
    • 1
    Email author
  • Leandro Paiola Albrecht
    • 1
  • Alfredo Junior Paiola Albrecht
    • 1
  • Victor José Salomão Cesco
    • 1
  • Danilo Morilha Rodrigues
    • 1
  • Roberto Luis Portz
    • 1
  • Luiz Henrique Saes Zobiole
    • 2
  1. 1.Federal University of Parana (UFPR)-Setor PalotinaPalotinaBrazil
  2. 2.Researcher Crop Protection R & D, Leading Research Projects for New Herbicides Dow Brasil, Dow AgrosciencesSao PauloBrazil

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