Acta Physiologiae Plantarum

, 39:57 | Cite as

Immunological evidence for the presence of peroxiredoxin in pea leaf peroxisomes and response to oxidative stress conditions

  • Francisco J. CorpasEmail author
  • José R. Pedrajas
  • José M. Palma
  • Raquel Valderrama
  • Marta Rodríguez-Ruiz
  • Mounira Chaki
  • Luis A. del Río
  • Juan B. Barroso
Original Article


Peroxiredoxins (Prxs) constitute a group of thiol-specific antioxidant enzymes which are present in bacteria, yeasts, and in plant and animal cells. Although Prxs are mainly localized in the cytosol, they are also present in mitochondria, chloroplasts, and nuclei, but there is no evidence of the existence of Prxs in plant peroxisomes. Using soluble fractions (matrices) of peroxisomes purified from leaves of pea (Pisum sativum L.) plants, the immunological analysis with affinity-purified IgG against yeast Prx1 revealed the presence of an immunoreactive band of about 50 kDa. The apparent molecular mass of the peroxisomal Prx was not sensitive to oxidizing and reducing conditions what could be a mechanism of protection against the oxidative environment existing in peroxisomes. Postembedment, EM immunocytochemical analysis with affinity-purified IgG against yeast Prx1 antibodies, confirmed that this protein was present in the peroxisomal matrix, mitochondria, and chloroplasts. In pea plants grown under oxidative stress conditions, the protein level of peroxisomal Prx was differentially modulated, being slightly induced by growth of plants with 50 µM CdCl2, but being significantly reduced by treatment with the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The presence in the matrix of peroxisomes of a protein immunorelated to Prx of about 50 kDa, which is in the range of molecular mass of the dimeric form of other Prxs, opens new questions on the molecular properties of Prxs, but also on their function in the metabolism of reactive oxygen and nitrogen species (ROS/RNS) in these plant cell organelles, where they could be involved in the regulation of hydrogen peroxide and/or peroxynitrite.


Peroxiredoxin Peroxisomes Pea leaves Pisum sativum Oxidative stress Nitrosative stress 



This work was supported by ERDF-cofinanced grants from the Ministry of Science and Innovation (BIO2012-33904) and Junta de Andalucía (research groups CVI 192 and CVI 286). Present research for FJC and JMP is supported by the ERDF-cofinanced grant AGL2015-65104-P and for JBB the BIO2015-66390-P grant both from the Ministry of Economy and Competitiveness (MINECO), Spain. The electron microscopy assays were carried out at the Centre of Scientific Instrumentation of the University of Granada. The valuable technical assistance of Mr. Carmelo Ruíz-Torres is acknowledged.

Supplementary material

11738_2017_2356_MOESM1_ESM.pptx (57 kb)
Supplementary material 1 (PPTX 56 kb)


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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2017

Authors and Affiliations

  • Francisco J. Corpas
    • 1
    Email author
  • José R. Pedrajas
    • 2
  • José M. Palma
    • 1
  • Raquel Valderrama
    • 2
  • Marta Rodríguez-Ruiz
    • 1
  • Mounira Chaki
    • 2
  • Luis A. del Río
    • 1
  • Juan B. Barroso
    • 2
  1. 1.Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of PlantsEstación Experimental del Zaidín, CSICGranadaSpain
  2. 2.Group of Biochemistry and Cell Signaling in Nitric Oxide, Department of Biochemistry and Molecular BiologyUniversity of JaénJaénSpain

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