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Antioxidant pool optimization in Carthamus tinctorius L. leaves under different NaCl levels and treatment durations

  • Sonia Zaoui
  • Hélène Gautier
  • Doriane Bancel
  • Ghaya Chaabani
  • Hanen Wasli
  • Mokhtar Lachaâl
  • Najoua Karray-Bouraoui
Original Article
  • 337 Downloads

Abstract

Safflower (Carthamus tinctorius L.) leaves are a good source of natural antioxidants, but their content is highly related to the plant response to NaCl constraint; therefore, optimizing culture conditions is required to improve leaf antioxidant accumulation while maintaining acceptable biomass production under stressful conditions. Leaves from plants grown at (0, 25, 50, and 75 mM NaCl) for three treatment durations (7, 15, and 30 days) were studied. NaCl concentrations and treatment durations for optimal biomass and biomolecules production were concluded. After 30 days of treatment, 25 mM NaCl enhanced total AsA leaf accumulation, while glutathione, soluble protein accumulation as well as SOD and GR activities were promoted at 75 mM NaCl. Non-significant changes were found for MDHAR and DHAR activity, added to, a very low level of reduced AsA, suggesting that ascorbate recycling was not an efficient system in safflower leaves under salinity. Our study demonstrates that C. tinctorius tolerates NaCl stress due to the complementary roles of powerful antioxidant molecules, such as ascorbate (for short-term treatments), glutathione (for long-term treatments), and increased SOD and GR activities.

Keywords

Safflower Salt stress Optimization Secondary metabolites Antioxidant enzyme activity Ascorbate recycling 

Abbreviations

AsA

Ascorbate

DHA

Dehydroascorbate

MDHA

Monodehydroascorbate

DHAR

Dehydroascorbate reductase

MDHAR

Monodehydroascorbate reductase

GSH

Glutathione

ROS

Reactive oxygen species

SOD

Superoxide dismutase

GR

Glutathione reductase

Notes

Acknowledgments

We the authors of this article do firstly thank the Tunisian Ministry of Scientific Research and Technology for its support. We secondly do express our grateful acknowledgement to Agreenium who funded the research activity and transport during the internship in INRA Avignon, France. We do also thank Rabeb Tej and Amel Hamdi for their technical support during the experiments and Sylvie Sérino for the instructions and discussion during ascorbate assays. We do finally heartily thank Mr Adel Khadhraoui for providing language help.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2016

Authors and Affiliations

  • Sonia Zaoui
    • 1
  • Hélène Gautier
    • 2
  • Doriane Bancel
    • 2
  • Ghaya Chaabani
    • 1
  • Hanen Wasli
    • 1
  • Mokhtar Lachaâl
    • 1
  • Najoua Karray-Bouraoui
    • 1
  1. 1.Unité de Physiologie et de Biochimie de la Réponse des Plantes aux Contraintes Abiotiques, Faculté des Sciences de TunisUniversité de Tunis El ManarTunisTunisia
  2. 2.INRA, UR1115, PSH, Plantes et Systèmes de Culture HorticolesAvignon Cedex 9France

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