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Acta Physiologiae Plantarum

, 37:252 | Cite as

Expression of ethylene biosynthetic and signaling genes in relation to ripening of banana fruit after cold storage

  • Keqian Hong
  • Jianghui Xie
  • Ru Zou
  • Ezhen Zhang
  • Min Xin
  • Maokang Huang
  • Quanguang HeEmail author
Original Article

Abstract

Banana fruit are highly sensitive to chilling injury (CI), while the effect of different degrees of CI on the subsequent fruit ripening is largely unknown. In the present work, ripening characteristic of banana fruit after storage at 7 °C for 3 days or for 8 days, and expression levels of eight genes associated with ethylene biosynthetic and signaling, including MaACS1, MaACO1, MaERS1, MaERS3, and MaEIL14, were investigated. The results showed that banana fruit stored at 7 °C for 8 days exhibited more severe chilling symptoms than those at 7 °C for 3 days. Compared with banana fruit stored at 7 °C for 8 days, which showed abnormal ripening, more decrease in fruit firmness, while higher increase in ethylene production and hue angle were observed in banana fruit stored at 7 °C for 3 days, which could ripening normally. Moreover, gene expression profiles during ripening revealed that ethylene biosynthetic and signaling genes were differentially expressed in peel and pulp of banana fruit after storage at 7 °C for 3 days and 7 °C for 8 days. In the peel of fruit storage at 7 °C for 3 days, expression levels of MaACS1, MaACO1, MaEIL1, and MaEIL2 increased remarkably while MaERS3, MaEIL1, and MaEIL4 were enhanced in the fruit after storage at 7 °C for 8 days. In the pulp, with the exception of MaACO1 and MaERS3, expression levels of other genes did not exhibit a significant difference, between the banana fruit storage at 7 °C for 3 days and 7 °C for 8 days. Taken together, our results suggest that differential expression of ethylene biosynthetic and signaling genes such as MaERS3, MaACO1, and MaEIL2, may be related to ripening behavior of banana fruit with different degrees of CI after cold storage.

Keywords

Banana fruit Chilling injury Ripening Ethylene biosynthetic and signaling genes 

Notes

Acknowledgments

This work was supported in part by the National Natural Science Foundation of China (grant no. 31160406) and Guangxi Natural Science Foundation (grant no. 2012GXNSFBA053058).

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2015

Authors and Affiliations

  • Keqian Hong
    • 1
  • Jianghui Xie
    • 1
  • Ru Zou
    • 4
  • Ezhen Zhang
    • 2
  • Min Xin
    • 2
  • Maokang Huang
    • 2
  • Quanguang He
    • 2
    • 3
    Email author
  1. 1.Key Laboratory for Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province, South Subtropical Crop Research InstituteChinese Academy of Tropical Agricultural SciencesZhanjiangChina
  2. 2.Institute of Agro-food Science and TechnologyGuangxi Academy of Agricultural SciencesNanningChina
  3. 3.Guangxi Crop Genetic Improvement LaboratoryNanningChina
  4. 4.BGI-ShenzenShenzhenChina

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