Acta Physiologiae Plantarum

, 37:237 | Cite as

The effect of methyl jasmonate on enzyme activities in wheat genotypes infected by the crown and root rot pathogen Fusarium culmorum

  • Parastoo Motallebi
  • Vahid Niknam
  • Hassan Ebrahimzadeh
  • Sattar Tahmasebi Enferadi
  • Majid Hashemi
Original Article
First Online:
Received:
Revised:
Accepted:

Abstract

The hemibiotrophic pathogen Fusarium culmorum (Fc) causes crown and root rot (CRR) in wheat. In this study, MeJA treatment was done 6 h after pathogen inoculation (hai) to focus the physiological and biochemical responses in root tissue of the susceptible wheat cv Falat, partially resistant cv Pishtaz and the tolerant cv Sumai3 at the beginning of the necrotrophic stage. The results indicate that treatment with MeJA at 6 hai significantly delayed the necrotic progress in cv Falat, whereas no significant difference was seen in other cultivars. The activities of pathogen responsive defense-related enzymes (SOD, CAT, POX, PPO, LOX and PAL), total phenols and callose contents were higher in Sumai3, while treatment with MeJA significantly increased these enzymes activities and total phenols content in Falat, signifying the most sensitive cultivar which had a weak reaction to the pathogen but a strong response to MeJA treatment. Additionally, MeJA treatment decreased the level of H2O2 and MDA contents particularly in cv Falat. This is the first work reporting the regulation of defense-related enzymes by MeJA treatment at particular time point of 6 hai suggests the possible role of JA in regulating basal resistance in CRR pathogen–wheat interaction. Taken together, our data add new insights into the mechanism of wheat defense including enzymatic events controlling wheat protection against Fc infection.

Keywords

Fusarium culmorum Defense responses Methyl jasmonate Necrotrophic stage Wheat 

Abbreviations

Fc

Fusarium culmorum

CRR

Crown and root rot

FHB

Fusarium head blight

JA

Jasmonic acid

MeJA

Methyl jasmonate

SOD

Superoxide dismutase

CAT

Catalase

POX

Peroxidases

PPO

Polyphenol oxidase

LOX

Lipoxygenase

PAL

Phenylalanine ammonia-lyase

H2O2

Hydrogen peroxide

MDA

Malondialdehyde

PCs

Phenolic compounds

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Notes

Acknowledgments

The financial support of this research was provided by University of Tehran. We thank the entire staff of School of Biology for their assistance in this experiment.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2015

Authors and Affiliations

  • Parastoo Motallebi
    • 1
  • Vahid Niknam
    • 1
  • Hassan Ebrahimzadeh
    • 1
  • Sattar Tahmasebi Enferadi
    • 2
  • Majid Hashemi
    • 3
  1. 1.Department of Plant Biology, and Center of Excellence in Phylogeny of Living Organisms, School of Biology, College of ScienceUniversity of TehranTehranIran
  2. 2.National Institute of Genetic Engineering and Biotechnology (NIGEB)TehranIran
  3. 3.Department of Plant PathologySeed and Plant Improvement InstituteKarajIran

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