Advertisement

Anther culture of Anthurium: a review

  • Jaime A. Teixeira da SilvaEmail author
  • Budi WinartoEmail author
  • Judit DobránszkiEmail author
  • Songjun ZengEmail author
Review

Abstract

The use of anther culture to produce haploid plants is a useful breeding technique for Anthurium, but has only seen limited applications thus far. This review describes the advances achieved thus far, and provides practical tips and protocols that would allow researchers new to the field, or already experienced Anthurium tissue culture scientists, to establish a new field of research, or a novel way to derive haploid plants for breeding programs. Anther culture can result in considerable somaclonal variation, but this has translated into a novel way of inducing new color variants of the spathe, the most marketable part of this ornamental plant. Considering this variability in vitro, anther culture is most likely not the most suitable method for clonal propagation of important germplasm, but is certainly a viable method to induce new genetically stable leaf and flower color variants.

Keywords

Anther culture Araceae Double-haploids Organogenesis Winarto-Teixeira medium 

Notes

Compliance with ethical standards

Conflict of interest

No authors have any conflicts of interest to declare.

References

  1. Aiti FJ, Benlhabib O, Sharma HC, El Jaafari S, El Hadrami I (1999) Genotypic variation in anther culture and effect of ovary coculture in durum wheat. Plant Cell Tissue Organ Cult 59:71–76CrossRefGoogle Scholar
  2. Bajaj YPS (1990) In vitro production of haploids and their use in cell genetics and plant breeding. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry. Part1. Haploids in crop improvement, vol 12. Springer, Berlin, pp 1–44CrossRefGoogle Scholar
  3. Bejoy M, Sumitha VR, Anish NP (2008) Foliar regeneration in Anthurium andreanum Hort. cv. Agnihothri. Biotechnology (Pakistan) 7(1):134–138CrossRefGoogle Scholar
  4. Callotte V (2004) Anthurium aristocracy. NZ Gard J 7(1):3–5Google Scholar
  5. Chen C, Hou XL, Zhang HX, Wang GL, Tian LM (2011) Induction of Anthurium andraeanum Arizona tetraploid by colchicine in vitro. Euphytica 181:137–145. doi: 10.1007/s10681-010-0344-3 CrossRefGoogle Scholar
  6. Custer JBM (2004) Preliminary research in anther culture of Anthurium. HAPLIN Report, Number 20. HORTIN Project, pp 1–6Google Scholar
  7. Datta SK (2005) Androgenic haploids: factors controlling development and its application in crop improvement. Curr Sci 89:1870–1878Google Scholar
  8. De Klerk GJ (2009) Adventitious regeneration. In: Flickinger MC (ed) Encyclopedia of industrial biotechnology: bioprocess, bioseparation, and cell technology, vol 1. Wiley, London, pp 72–87Google Scholar
  9. Du BG, Huang LJ, Zhang ZS, Li YH, Liang CH, Yi MS (2009) Anther culture of Anthurium andraeanum Linden. Biotechnol Bull Supplement Issue:189–195Google Scholar
  10. Dufour L, Guérin V (2006) Main environmental factors affecting flowering of Anthurium. In: Teixeira da Silva JA (ed) Floriculture, ornamental and plant biotechnology: advances and topical issues, vol III. Global Science Books, Ltd., Isleworth, pp 172–182Google Scholar
  11. Eeckhaut T, Werbrouck S, Dendauw J, Bockstaele EV, Debergh P (2001) Induction of homozygous Spathiphyllum wallisii genotypes through gynogenesis. Plant Cell Tissue Org Cult 67:181–189CrossRefGoogle Scholar
  12. Geier T (1990) Anthurium. In: Amirato PV, Evans DA, Sharp WR, Bajaj YPS (eds) Handbook of plant cell culture, ornamental species, vol 5. McGraw-Hill, New York, pp 228–252Google Scholar
  13. Germanà MA (2011) Anther culture for haploid and doubled haploid production. Plant Cell Tissue Organ Cult 104:283–300. doi: 10.1007/s11240-010-9852-z CrossRefGoogle Scholar
  14. Grafi G (2004) How cells dedifferentiate: a lesson from plants. Dev Biol 268:1–6. doi: 10.1016/j.ydbio.2003.12.027 CrossRefPubMedGoogle Scholar
  15. Guha S, Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature 204:497CrossRefGoogle Scholar
  16. Higaki T, Lichty JS, Moniz D (1995) Anthurium culture in Hawai’i. University of Hawaii, HITAHR Res. Extension Ser. 152, Honolulu, p 28Google Scholar
  17. Jahan MT, Islam MR, Khan R, Mamun ANK, Ahmed G, Hakim L (2009) In vitro clonal propagation of anthurium (Anthurium andraeanum L.) using callus culture. Plant Tissue Cult Biotech 19(1):61–69. doi: 10.3329/ptcb.v19i1.4961 Google Scholar
  18. Jain SM, Sopory SK, Vielleux RE (1996) In viro haploid production in higher plants, vol 1. Kluwer Academic Publishers, Netherlands, pp 145–176Google Scholar
  19. Lichter R (1982) Induction of haploid plants from isolated pollen of Brassica napus. Z Pflanzenphysiol 105:427–434CrossRefGoogle Scholar
  20. Maluszynski M, Kasha KJ, Forster BP, Szarejsko I (2003) Double haploid production in crop plants: a manual. Kluwer Academic Publishers, DordrechtCrossRefGoogle Scholar
  21. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–479CrossRefGoogle Scholar
  22. Rachmawati F (2007) Anther culture on anthurium (Anthurium andraeanum Linden ex. Andre). MSc thesis, Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agriculture Institute, Bogor, IndonesiaGoogle Scholar
  23. Smulders MJM, De Klerk GJ (2011) Epigenetics in plant tisuue culture. Plant Growth Regul 63:137–146. doi: 10.1007/s10725-010-9531-4 CrossRefGoogle Scholar
  24. Teixeira da Silva JA, Dobránszki J, Winarto B, Zeng S-J (2015a) Anthurium in vitro: a review. Sci Hortic 186:266–298. doi: 10.1016/j.scienta.2014.11.024 CrossRefGoogle Scholar
  25. Teixeira da Silva JA, Winarto B, Dobránszki J, Zeng S-J (2015b) Disinfection procedures for in vitro propagation of Anthurium. Folia Hortic 27(1):3–14. doi: 10.1515/fhort-2015-0009 Google Scholar
  26. Tian LM, Ma YL (2008) Polyploid induction and ploidy analysis of Anthurium andraenum Linden. J Anhui Agric Sci 36(32):13977–13978, 13983. doi: 10.13989/j.cnki.0517-6611.2008.32.113
  27. Tian DQ, Pan XY, Ge YY, Liu XJ, Pan GM (2013) In vitro tetraploid induction of Anthurium andraeanum by colchicine. Jiejiang Agric Sci Technol 9:1125–1127Google Scholar
  28. Us-Camas R, Rivera-Solís G, Duarte-Aké F, De-la-Peña C (2014) In vitro culture: an epigenetic challenge for plants. Plant Cell Tissue Organ Cult 118:187–201. doi: 10.1007/s11240-014-0482-8 CrossRefGoogle Scholar
  29. Wang SM, Li XL, Yang LL, Wu HZ, Zheng SX, Zhang XY, Zhang LM (2011) Microspore culture of Zantedeschia aethiopica: the role of monosaccharides in sporophytic development. Afr J Biotechnol 10(50):10287–10292. doi: 10.5897/AJB11.704 Google Scholar
  30. Winarto B (2009) Androgenesis: a breakthrough effort for preparing haploid or double-haploid plants in Anthurium. PhD Dissertation, Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agriculture InstituteGoogle Scholar
  31. Winarto B (2010) Improving growth and regeneration of explants derived from anther culture of Anthurium via improving culture media. J Hortic 20(4):342–351Google Scholar
  32. Winarto B (2011a) Effect of glumatine and serine on anther culture of Anthurium andreanum cv. ‘Tropical’. J Hortic 21(4):295–305Google Scholar
  33. Winarto B (2011b) Chromosome staining and its application on determining ploidy level of plantlets derived from anther culture of Anthurium. J Hortic 21(2):113–123Google Scholar
  34. Winarto B (2013) Effect of basic medium and ammonium nitrate on formation and regeneration of calli and shoot multiplication derived from anther culture of anthurium. J Hortic 23(1):9–20Google Scholar
  35. Winarto B (2014) Anther culture study on Anthurium andreanum cv. Casino, Laguna and Safari. Proc. Nat. Floric. Sem. Auditorium of Indonesian Ornamental Crop Research Institute, Pacet-Cianjur, West Java-Indonesia, 29 August 2013 and published 2014, pp 271–281Google Scholar
  36. Winarto B, Mattjik NA (2009a) Response of Anthurium andreanum Linden ex André cv. Carnaval anther in medium with various combinations of plant growth regulator concentrations. J Agron Indones 37(2):138–144Google Scholar
  37. Winarto B, Mattjik NA (2009b) Studies on anther culture of local accessions of Anthurium. J Hortik 19(4):386–395Google Scholar
  38. Winarto B, Rachmawati F (2007) Preliminary study of anther culture of Anthurium. J Hortic 17(2):127–137Google Scholar
  39. Winarto B, Teixeira da Silva JA (2012) Influence of isolation technique of half-anthers and of initiation culture medium on callus induction and regeneration in Anthurium andreanum. Plant Cell Tissue Organ Cult 110(3):401–411. doi: 10.1007/s11240-012-0161-6 CrossRefGoogle Scholar
  40. Winarto B, Hayati NA, Purwito A, Marwoto B (2009) Effect of sucrose and glucose on successful induction and regeneration of callus derived from anther culture of Anthurium. J Biol Res 14(2):165–172Google Scholar
  41. Winarto B, Hayati NA, Purwito A, Marwoto B (2010a) Improvement of selected induction media on callus induction in anther culture of Anthurium and a histological study on its callus formation. J Nat Indones 12(2):93–101Google Scholar
  42. Winarto B, Hayati NA, Purwito A, Marwoto B (2010b) Application of 2,4-D and TDZ in formation and regeneration of callus derived from anther culture of anthurium. J Hortic 20(1):1–9Google Scholar
  43. Winarto B, Mattjik NA, Purwito A, Marwoto B (2010c) Improvement of selected induction culture media on callus induction in anther culture of Anthurium and a histological study on its callus formation. J Nat Indones 12(2):93–101Google Scholar
  44. Winarto B, Mattjik NA, Teixeira da Silva JA, Purwito A, Marwoto B (2010d) Ploidy screening of anthurium (Anthurium andreanum Linden ex André) regenerants derived from anther culture. Sci Hortic 127:86–90. doi: 10.1016/j.scienta.2010.09.004 CrossRefGoogle Scholar
  45. Winarto B, Rachmawati F, Pramanik D, Teixeira da Silva JA (2011a) Morphological and cytological diversity of regenerants derived from Anthurium anther culture. Plant Cell Tissue Organ Cult 105(3):363–374. doi: 10.1007/s11240-010-9876-4 CrossRefGoogle Scholar
  46. Winarto B, Rachmawati F, Teixeira da Silva JA (2011b) New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical. Plant Growth Regul 65(3):513–529. doi: 10.1007/s10725-011-9622-x CrossRefGoogle Scholar
  47. Yang X, Wang L, Yuan D, Lindsey K, Zhang X (2013) Small DNA and degradome sequencing reveal complex miRNA regulation during cotton somatic embryogenesis. J Exp Bot 64:1521–1536. doi: 10.1093/jxb/ert013 PubMedCentralCrossRefPubMedGoogle Scholar
  48. Zhang ZS, Li YF, Jiang L, Li Y, Wang ZY, Xia J, Yi MS (2007) In vitro tetraploid induction and its identification in Anthurium andraeanum. Acta Hortic Sin 34(3):729–734Google Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2015

Authors and Affiliations

  1. 1.IkenobeJapan
  2. 2.Tissue Culture DepartmentIndonesian Ornamental Crops Research InstitutePacet-CianjurIndonesia
  3. 3.Research Institute of NyíregyházaUniversity of DebrecenNyíregyházaHungary
  4. 4.Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical GardenChinese Academy of SciencesGuangzhouChina

Personalised recommendations