Acta Physiologiae Plantarum

, 37:32 | Cite as

Effect of exogenous salicylic acid on manganese toxicity, mineral nutrients translocation and antioxidative system in polish wheat (Triticum polonicum L.)

  • Huajin Sheng
  • Jian Zeng
  • Fei Yan
  • Xiaolu Wang
  • Yi Wang
  • Houyang Kang
  • Xing Fan
  • Lina Sha
  • Haiqin Zhang
  • Yonghong ZhouEmail author
Original Paper


The present study investigated the morphological and physiological effect of salicylic acid (SA) on manganese toxicity in dwarf polish wheat (Triticum polonicum L.) seedlings grown hydroponically. Our findings showed that Mn stress could decrease plant growth, cause serious chlorosis and injury the photosynthetic apparatus. An increase of Mn accumulation and the inhibition of the K and Ca absorption and the Mg, Fe and Zn translocation were observed under Mn stress. Also, there was a considerable increase in H2O2 and TBARS (thiobarbituric acid-reactive substances) content in both the roots and leaves under Mn condition. The combination of SA and Mn treatment decreased the transport of Mn, Fe and Zn from roots to shoots and increased the Ca absorption and Mg translocation. In antioxidant system, such as CAT, APX, GR, DHAR, GSH and AsA, the combined treatment significantly increased the antioxidant content and antioxidative enzyme activities compared to the Mn stress alone. The level of ROS and lipid peroxidation significantly decreased under the combination of SA and Mn. These results suggested that SA-induced Mn tolerances in polish wheat are mainly by inhibiting Mn translocation, enhancing enzymatic activities and nonenzymatic antioxidants contents, and regulating nutrient absorption and distribution in plants.


Salicylic acid Mn stress Wheat plants Physiological characteristics Oxidative stress Antioxidants Mineral elements 



Ascorbate peroxidase






Dehydroascorbate reductase


Glutathione reductase






Reactive oxygen species


Salicylic acid


Superoxide dismutase


Thiobarbituric acid-reactive substances



The authors thank the National Natural Science Foundation of China (No. 31301349, 30870154, 30901052, 30900087), Bureau of Science and Technology and Bureau of Education of Sichuan Province, China.


  1. Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126CrossRefPubMedGoogle Scholar
  2. Ait Ali N, Bernal MP, Ater M (2004) Tolerance and bioaccumulation of cadmium by Phragmites australis grown in the presence of elevated concentrations of cadmium, copper, and zinc. Aquat Bot 80:163–176CrossRefGoogle Scholar
  3. Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399CrossRefPubMedGoogle Scholar
  4. Arya SK, Roy BK (2011) Manganese induced changes in growth, chlorophyll content and antioxidants activity in seedlings of broad bean (Vicia faba L.). J Environ Biol 32:707–711PubMedGoogle Scholar
  5. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287CrossRefPubMedGoogle Scholar
  6. Belkhadi A, Hediji H, Abbes Z, Nouairi I, Barhoumi Z, Zarrouk M, Djebali W (2010) Effects of exogenous salicylic acid pre-treatment on cadmium toxicity and leaf lipid content in Linum usitatissimum L. Ecotoxicol Environ Safe 73:1004–1011CrossRefGoogle Scholar
  7. Bowler C, Fluhr R (2000) The role of calcium and activated oxygens as signals for controlling cross-tolerance. Trends Plant Sci 5:241–246CrossRefPubMedGoogle Scholar
  8. Cheng H, Wang M, Wong MH, Ye Z (2014) Does radial oxygen loss and iron plaque formation on roots alter Cd and Pb uptake and distribution in rice plant tissues? Plant Soil 375:137–148CrossRefGoogle Scholar
  9. Cobbett CS (2000) Phytochelatins and their roles in heavy metal detoxification. Plant Physiol 123:825–832CrossRefPubMedCentralPubMedGoogle Scholar
  10. Drazic G, Mihailovic N (2005) Modification of cadmium toxicity in soybean seedlings by salicylic acid. Plant Sci 168:511–517CrossRefGoogle Scholar
  11. Foyer CH, Halliwell B (1976) The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta 133:21–25CrossRefPubMedGoogle Scholar
  12. Gordon LK, Minibayeva FV, Rakhmatullina DF, Alyabyev AJ, Ogorodnikova TI, Loseva NL, Valitova YN (2004) Heat production of wheat roots induced by the disruption of proton gradient by salicylic acid. Thermochim Acta 422:101–104CrossRefGoogle Scholar
  13. Guo B, Liang YC, Zhu YG, Zhao FJ (2007) Role of salicylic acid in alleviating oxidative damage in rice roots (Oryza sativa) subjected to cadmium stress. Environ Pollut 147:743–749CrossRefPubMedGoogle Scholar
  14. Hall JL (2002) Cellular mechanisms for heavy metal detoxification and tolerance. J Exp Bot 53:1–11CrossRefPubMedGoogle Scholar
  15. Hayat Q, Hayat S, Irfan M, Ahmad A (2010) Effect of exogenous salicylic acid under changing environment: a review. Environ Exp Bot 68:14–25CrossRefGoogle Scholar
  16. Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198CrossRefPubMedGoogle Scholar
  17. Hossain MA, Piyatida P, da Silva JAT, Fujita M (2012) Molecular mechanism of heavy metal toxicity and tolerance in plants: central role of glutathione in detoxification of reactive oxygen species and methylglyoxal and in heavy metal chelation. J Bot 2012:1–17CrossRefGoogle Scholar
  18. Knörzer OC, Burner J, Boger P (1996) Alterations in the antioxidative system of suspension-cultured soybean cells (Glycine max) induced by oxidative stress. Physiol Plant 97:388–396CrossRefGoogle Scholar
  19. Knudson LL, Tibbitts TW, Edwards GE (1977) Measurement of ozone injury by determination of leaf chlorophyll concentration. Plant Physiol 60:606–608CrossRefPubMedCentralPubMedGoogle Scholar
  20. Li Q, Chen LS, Jiang HX, Tang N, Yang LT, Lin ZH, Yang GH (2010) Effects of manganese-excess on CO2 assimilation, ribulose-1, 5-bisphosphate carboxylase/oxygenase, carbohydrates and photosynthetic electron transport of leaves, and antioxidant systems of leaves and roots in Citrus grandis seedlings. BMC Plant Biol 10:42CrossRefPubMedCentralPubMedGoogle Scholar
  21. Li P, Song A, Li Z, Fan F, Liang Y (2012) Silicon ameliorates manganese toxicity by regulating manganese transport and antioxidant reactions in rice (Oryza sativa L.). Plant Soil 354:407–419CrossRefGoogle Scholar
  22. Lidon FC, Barreiro MG, Ramalho JC (2004) Manganese accumulation in rice: implications for photosynthetic functioning. J Plant Physiol 161:1235–1244CrossRefPubMedGoogle Scholar
  23. Macfie SM, Taylor GJ (1992) The effects of excess manganese on photosynthetic rate and concentration of chlorophyll in Triticum aestivum grown in solution culture. Physiol Plant 85:467–475CrossRefGoogle Scholar
  24. Malamy J, Carr JP, Klessig DF, Raskin I (1990) Salicylic acid: a likely endogenous signal in the resistance response of tobacco to viral infection. Science 250:1002–1004CrossRefPubMedGoogle Scholar
  25. Marschner H, Rimmington GM (1988) Mineral nutrition of higher plants. Plant Cell Environ 11:147–148Google Scholar
  26. Millaleo R, Reyes-Diaz M, Ivanov A, Mora M, Alberdi M (2010) Manganese as essential and toxic element for plants: transport, accumulation and resistance mechanisms. J Soil Sci Plant Nutr 10:470–481CrossRefGoogle Scholar
  27. Mostofa MG, Fujita M (2013) Salicylic acid alleviates copper toxicity in rice (Oryza sativa L.) seedlings by up-regulating antioxidative and glyoxalase systems. Ecotoxicology 22:959–973CrossRefPubMedGoogle Scholar
  28. Nagalakshmi N, Prasad MNV (2001) Responses of glutathione cycle enzymes and glutathione metabolism to copper stress in Scenedesmus bijugatus. Plant Sci 160:291–299CrossRefPubMedGoogle Scholar
  29. Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar
  30. Ren LM, Liu P, Cai MZ, Xu GD, Fang XY, Cheng ZX (2007) Physiological response of polygonum hydropiper, comnyza canadensis, polygonum perfoliatum and phytolacca americana to manganese toxicity. J Soil Water Conser 21:81–85Google Scholar
  31. Seregin IV, Kozhevnikova AD (2006) Physiological role of nickel and its toxic effects on higher plants. Russ J Plant Physiol 53:257–277CrossRefGoogle Scholar
  32. Shah K, Kumar RG, Verma S, Dubey RS (2001) Effect of cadmium on lipid peroxidation, superoxide anion generation and activities of antioxidant enzymes in growing rice seedlings. Plant Sci 161:1135–1144CrossRefGoogle Scholar
  33. Sharma SS, Dietz KJ (2009) The relationship between metal toxicity and cellular redox imbalance. Trends Plant Sci 14:43–50CrossRefPubMedGoogle Scholar
  34. Shi Q, Zhu Z (2008) Effects of exogenous salicylic acid on manganese toxicity, element contents and antioxidative system in cucumber. Environ Exp Bot 63:317–326CrossRefGoogle Scholar
  35. Subrahmanyam D, Rathore VS (2001) Influence of manganese toxicity on photosynthesis in ricebean (Vigna umbellata) seedlings. Photosynthetica 38:449–453CrossRefGoogle Scholar
  36. Suresh R, Foy CD, Weidner JR (1987) Effects of excess soil manganese on stomatal function in two soybean cultivars. J Plant Nutr 10:749–760CrossRefGoogle Scholar
  37. Wang C, Zhang S, Wang P, Hou J, Qian J, Ao Y, Li L (2011) Salicylic acid involved in the regulation of nutrient elements uptake and oxidative stress in Vallisneria natans (Lour.) Hara under Pb stress. Chemosphere 84:136–142CrossRefPubMedGoogle Scholar
  38. Wang Q, Liang X, Dong Y, Xu L, Zhang X, Kong J, Liu S (2013) Effects of exogenous salicylic acid and nitric oxide on physiological characteristics of perennial ryegrass under cadmium stress. J Plant Growth Regul 32:721–731CrossRefGoogle Scholar
  39. Wiwart M, Suchowilska E, Kandler W, Sulyok M, Groenwald P, Krska R (2013) Can polish wheat (Triticum polonicum L.) be an interesting gene source for breeding wheat cultivars with increased resistance to fusarium head blight ? Genet Resour Crop Evol 60:2359–2373CrossRefGoogle Scholar
  40. Zhang F, Zhang H, Xia Y, Wang G, Xu L, Shen Z (2011) Exogenous application of salicylic acid alleviates cadmium toxicity and reduces hydrogen peroxide accumulation in root apoplasts of Phaseolus aureus and Vicia sativa. Plant Cell Rep 30:1475–1483CrossRefPubMedGoogle Scholar
  41. Zhou ZS, Guo K, Elbaz AA, Yang ZM (2009) Salicylic acid alleviates mercury toxicity by preventing oxidative stress in roots of Medicago sativa. Environ Exp Bot 65:27–34CrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2015

Authors and Affiliations

  • Huajin Sheng
    • 1
  • Jian Zeng
    • 2
  • Fei Yan
    • 2
  • Xiaolu Wang
    • 1
  • Yi Wang
    • 1
  • Houyang Kang
    • 1
  • Xing Fan
    • 1
  • Lina Sha
    • 1
  • Haiqin Zhang
    • 1
  • Yonghong Zhou
    • 1
    Email author
  1. 1.Triticeae Research InstituteSichuan Agricultural UniversityWenjiangChina
  2. 2.Institute of Ecological and Environmental SciencesSichuan Agricultural UniversityWenjiangChina

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