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Responses of canola (Brassica napus L.) cultivars under contrasting temperature regimes during early seedling growth stage as revealed by multiple physiological criteria

  • Jing Zhang
  • Fangwei Jiang
  • Ping Yang
  • Jing Li
  • Guijun YanEmail author
  • Liyong HuEmail author
Original Paper

Abstract

Investigations were carried out to study the responses of canola (Brassica napus) under contrasting temperature regimes (day/night °C): 35/30 (high temperature) and 15/10 (low temperature) in comparison with optimal temperature (25/20) at early seedling stage. The results indicated that high temperature inhibited seedling establishment, while low temperature restrained seed germination. Both high and low temperatures showed detrimental effects on seedling growth as revealed by reduced establishment percentage, seedling vigor index, and fresh weight due to accumulated reactive oxygens. The antioxidant enzymes responded to high and low temperature differently. Under high temperature, superoxide dismutase (SOD) and peroxidase (POD) activities reduced, while catalase (CAT) activities increased. Under low temperature, however, SOD activities increased, while POD activities reduced, with CAT activities unchanged. Proline played an important role in temperature stress tolerance and can be used as an indicator for tolerance to unfavorable temperature conditions in canola seedlings. Huayouza 9 showed much stronger tolerance to both high and low temperature compared with other cultivars tested in this study. The full extent of tolerance mechanisms need to be further studied.

Keywords

Canola Temperature Germination Early seedling growth Antioxidant enzymes 

Abbreviations

CAT

Catalase

Chl

Chlorophyll

EP

Establishment percentage

FGP

Final germination percentage

FW

Fresh weight

POD

Peroxidase

Pro

Proline

SOD

Superoxide dismutase

SVI

Seedling vigor index

Notes

Acknowledgments

The research was funded by the National Natural Science Foundation of China (31271664) and National Key Technology Research and Development Program (2010BAD01B01). The authors sincerely thank the graduate students Mei Yan, Hongyu Zheng and Hongkun Peng from Huazhong Agricultural University for their help during this study.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2015

Authors and Affiliations

  1. 1.Ministry of Agriculture (MOA) Key Laboratory of Huazhong Crop Physiology, Ecology and Production, College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.School of Plant Biology, Faculty of Science and The UWA Institute of AgricultureThe University of Western AustraliaCrawleyAustralia

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