Acta Physiologiae Plantarum

, Volume 36, Issue 12, pp 3231–3241 | Cite as

Olive (Olea europaea L.) freezing tolerance related to antioxidant enzymes activity during cold acclimation and non acclimation

  • Abuzar Hashempour
  • Mahmood Ghasemnezhad
  • Reza Fotouhi Ghazvini
  • Mohammad Mehdi Sohani
Original Paper


The changes in the antioxidant enzymes activity, total protein and proline content and their correlations with freezing tolerance (FT) (expressed as LT50) were investigated at 11 different olive cultivars at cold-acclimation (CA, in February) and non-acclimation (NA, in August) stages. Leaf samples were collected from each cultivar and were divided into two groups. The first group was immediately frozen in liquid nitrogen for further biochemical analysis. The second ones was subjected to different freezing temperatures (−5, −10, −15 and −20 °C) for 10 h, in order to determine their FT. The unfrozen control samples were kept at 4 °C. The results showed that Fishomi, Mission and Shengeh were the most freezing tolerant among other cultivars. In contrast, Zard, Manzanilla and Amigdalolia were the most sensitive ones. The cold acclimation enhanced the activities of superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), catalase (CAT), polyphenol oxidase (PPO) and total protein content. However, proline content and phenylalanine ammonia-lyase (PAL) activity did not change or even decreased slightly at CA stage, compare to those samples at NA stage. It was found that LT50 to be closely correlated to POD, CAT, and PPO activity at CA and NA stages. Overall, higher leaf POD, CAT, and PPO activity could be used as important selection criteria in screening tolerant olive cultivars for cold zone climatic.


Olea europaea L. Freezing tolerance Acclamation Antioxidant enzymes 



The authors wish to express sincere gratitude to the the staffs of Olive Research Station of Roudbar, especialy Dr. Ramezani malkroudi for providing plant materials.


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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2014

Authors and Affiliations

  • Abuzar Hashempour
    • 1
  • Mahmood Ghasemnezhad
    • 1
  • Reza Fotouhi Ghazvini
    • 1
  • Mohammad Mehdi Sohani
    • 2
  1. 1.Department of Horticultural Sciences, Faculty of Agriculture SciencesUniversity of GuilanRashtIran
  2. 2.Department of Biotechnology, Faculty of Agriculture SciencesUniversity of GuilanRashtIran

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