Acta Physiologiae Plantarum

, Volume 36, Issue 8, pp 1981–1991 | Cite as

Multiple, concentration-dependent effects of sucrose, auxins and cytokinins in explant cultures of kale and tobacco

  • Jiří Luštinec
  • Fatima Cvrčková
  • Jana Čížková
  • Jaroslav Doležel
  • Miroslav Kamínek
  • Viktor Žárský
Original Paper

Abstract

We describe complex multiple concentration dependencies for the response of isolated pith tissues to plant biologically active substances. Kale and tobacco stem pith explants were cultured on agar media containing combinations of sucrose, cytokinin [kinetin or benzyladenine (BA)] and auxin [indole-3-acetic acid (IAA) or naphthaleneacetic acid (NAA)]. Absorption of these components by explants and their effects on explant mass, contents of soluble proteins, starch and sugars, and activity of ADP-glucose pyrophosphorylase (AGPase) were studied in relation to their concentration. Up to ten pronounced statistically significant maxima (peaks or waves) were repeatedly detected in the dose–response curves over a concentration range of several logarithmic orders. Slight maxima were observed in the corresponding absorption curves. Pronounced maxima of sucrose absorption were induced by IAA and BA, and those of NAA absorption were induced by sucrose. Both types of multiple maxima (in dose–response and absorption curves) may be due to changes in concentration of intracellular solutes (sugars, auxins and cytokinins), thereby affecting metabolic processes that act as sinks for external solutes and elicit feedback appearance of maxima in absorption curves. Good correspondence between external concentrations at which maxima of different compared curves occur in addition to statistical significance of individual maxima and repeatability of experimental results supports the conclusion that the multiple maxima exhibited are genuine. We consider it possibile that the multiple maxima are associated with endopolyploidy or mixoploidy and/or epigenomic diversity of pith cells that show different sensitivities to biologically active solutes.

Keywords

Brassica oleracea Nicotiana tabacum Absorption AGPase Endopolyploidy  Epigenomic diversity Mixoploidy Soluble proteins Starch accumulation Sugars 

Notes

Acknowledgments

The authors thank Prof. Per Nissen for encouragement and support, Mrs Anna Bastrová and Ing. Lenka Preclíková for technical assistance and Dr. Petr Cápal for informations concerning genomic diversification in cell cultures.

References

  1. Boerjan W, Genetello C, Van Montanu M, Inzé D (1992) A new bioassay for auxins and cytokinins. Plant Physiol 99:1090–1098PubMedCentralPubMedCrossRefGoogle Scholar
  2. Bradford MH (1972) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye-binding. Anal Biochem 72:248–254CrossRefGoogle Scholar
  3. De Veylder L, Larkin JC, Schnittger A (2011) Molecular control and function of endoreplication in development and physiology. Trends Plant Sci 16:626–634CrossRefGoogle Scholar
  4. Doležel J, Greilhuber J, Suda J (2007) Estimation of nuclear DNA content in plants using flow cytometry. Nat Protoc 2:2233–2244PubMedCrossRefGoogle Scholar
  5. Dubois M, Gilles KM, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356CrossRefGoogle Scholar
  6. Gibson S (2004) Sugar and phytohormone pathways: navigating a signalling network. J Exp Bot 55:253–264PubMedCrossRefGoogle Scholar
  7. Horák J, Landa Z, Luštinec J (1971) Production of polyploid plants from tissue cultures of Brassica oleracea L. Phyton 28:7–10Google Scholar
  8. Horák J, Luštinec J, Měsíček J, Kamínek M, Poláčková D (1975) Regeneration of diploid and polyploid plants from stem pith explants of diploid marrow stem kale (B. oleracea L.). Ann Bot 39:571–577Google Scholar
  9. Hylton C, Smith AM (1992) The rb mutation of peas causes structural and regulatory changes in ADP glucose pyrophosphorylase from developing embryos. Plant Physiol 99:1626–1634PubMedCentralPubMedCrossRefGoogle Scholar
  10. Ibraheem O, Hove RM, Bradley G (2008) Sucrose assimilation and the role of sucrose transporters in plant wound response. Afr J Biotech 7:4850–4854Google Scholar
  11. Keeling PL, Myers AM (2010) Biochemistry and genetics of starch synthesis. Annu Rev Food Sci Technol 1:271–303PubMedCrossRefGoogle Scholar
  12. Koch K (2004) Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. Curr Opin Plant Biol 7:235–246PubMedCrossRefGoogle Scholar
  13. Koshland DE Jr, Goldbeter A, Stock JB (1982) Amplification and adaptation in regulatory and sensory systems. Science 217:220–225PubMedCrossRefGoogle Scholar
  14. Kudo N, Kimura Y (2001) Flow cytometric evidence for endopolyploidy in seedlings of some Brassica species. Theor Appl Genet 102:104–110CrossRefGoogle Scholar
  15. Kutík J, Beneš K (1979) Structural aspects of the regulation of starch accumulation in stem pith explants of kale. Biol Plant 21:351–354CrossRefGoogle Scholar
  16. Luštinec J (1988) Uptake and dose responses of auxin in vitro: multiphasic concentration-dependencies. In: Kutáček M, Bandurski RS, Krekule J (eds) Physiology and biochemistry of auxins in plants. Academia, Praha, pp 241–246Google Scholar
  17. Luštinec J, Hadačová V, Kamínek M (1974) The effect of various cytokinins and auxins on starch formation in kale and tobacco explants. In: Schreiber K, Schütte HR, Sembdner G (eds) Biochemistry and chemistry of plant growth regulators. Inst Biochem Pflanz, Halle, pp 311–313Google Scholar
  18. Luštinec J, Kamínek M, Hadačová V (1976) Hormonal control of starch accumulation and cell expansion in tobacco stem pith. Acta Univ Nicolai Copernici 18:117–120Google Scholar
  19. Luštinec J, Hadačová V, Kamínek M, Procházka Ž (1983) Quantitative determination of starch, amylose and amylopectin in plant tissue using glass fiber paper. Anal Biochem 132:265–271PubMedCrossRefGoogle Scholar
  20. Luštinec J, Kamínek M, Beneš K, Conrad K (1984) Hormone-like effect of vascular tissue on starch accumulation in stem explants of kale, B. oleracea. Physiol Plant 61:224–230CrossRefGoogle Scholar
  21. Miguel C, Marum L (2011) An epigenetic view of plant cells cultured in vitro: somaclonal variation and beyond. J Exp Bot 62:3713–3725PubMedCrossRefGoogle Scholar
  22. Miller CO (1961) Kinetin and related compounds in plant growth. Annu Rev Plant Physiol 12:395–408CrossRefGoogle Scholar
  23. Murashige T, Nakano R (1966) Tissue culture as a potential tool in obtaining polyploid plants. J Hered 57:114–118Google Scholar
  24. Murashige T, Nakano R (1967) Chromosome complement as a determinant of the morphogenic potential of tobacco cells. Am J Bot 54:963–970CrossRefGoogle Scholar
  25. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497CrossRefGoogle Scholar
  26. Nissen P (1974) Uptake mechanisms: inorganic and organic. Annu Rev Plant Physiol 25:53–79CrossRefGoogle Scholar
  27. Nissen P (1985) Dose responses of auxins. Physiol Plant 65:357–374CrossRefGoogle Scholar
  28. Nissen P (1988) Dose responses of cytokinins. Physiol Plant 74:450–456CrossRefGoogle Scholar
  29. Nissen P (1991) Multiphasic uptake mechanisms in plants. Int Rev Cytol 126:89–134CrossRefGoogle Scholar
  30. Rolland F, Baena-Gonzalez E, Sheen J (2006) Sugar sensing and signalling in plants: conserved and novel mechanisms. Annu Rev Plant Biol 57:675–709PubMedCrossRefGoogle Scholar
  31. Scaramuzzi F, Palubo R, De Gaetano A (1973) Interaction of sucrose and naphthaleneacetic acid on rhizogenesis and callogenesis of stem segments of Viburnum lantana L. cultured in vito in the dark and in the light, vol 277. C R Academic Science, Paris, pp 493–496Google Scholar
  32. Schistad IJ, Nissen P (1984) Cytokinin-induced retention of chlorophyll in senescing barley leaves: complexity of dose response. Physiol Plant 61:566–570CrossRefGoogle Scholar
  33. Smeekens S, Ma J, Hanson J, Rolland F (2010) Sugar signals and molecular network controlling plant growth. Curr Opin Plant Biol 13:274–279PubMedCrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2014

Authors and Affiliations

  • Jiří Luštinec
    • 1
  • Fatima Cvrčková
    • 2
  • Jana Čížková
    • 3
  • Jaroslav Doležel
    • 3
  • Miroslav Kamínek
    • 1
  • Viktor Žárský
    • 1
    • 2
  1. 1.Institute of Experimental BotanyAcademy of Sciences of the Czech RepublicPrague 6Czech Republic
  2. 2.Department of Experimental Plant Biology, Faculty of ScienceCharles UniversityPrague 2Czech Republic
  3. 3.Institute of Experimental Botany, Centre of Plant Structural and Functional GenomicsAcademy of Sciences of the Czech RepublicOlomoucCzech Republic

Personalised recommendations