Acta Physiologiae Plantarum

, Volume 36, Issue 6, pp 1529–1537 | Cite as

Determination of genomic instability and DNA methylation effects of Cr on maize (Zea mays L.) using RAPD and CRED-RA analysis

  • Filiz Aygun Erturk
  • Guleray Agar
  • Esra Arslan
  • Gokce Nardemir
  • Zehra Sahin
Original Paper
First Online:
Received:
Revised:
Accepted:

Abstract

In this research, we aimed to evaluate DNA damage levels, DNA methylation, protein and phytohormone levels changes in corn (Zea mays L.) seedlings exposed to chromium nitrate (Cr). The results showed that the used all chromium doses caused a decreasing mitotic index, genomic template stability and soluble protein levels and increasing in RAPDs profile changes (DNA damage) and DNA hypermethylation. In additional, in the high-pressure liquid chromatography analyses, it was also observed that Cr contamination caused a decreasing in the growth-promoting hormones including gibberellic acid (GA), zeatin (ZA) and indole acetic acid (IAA) levels, in contrast to abscisic acid (ABA) level. The results of this experiment have clearly shown that Cr has epigenetic effect as well as genotoxic effect. Some of phytohormones decreasing (GA, ZA and IAA) and especially increasing of ABA levels under Cr stress may be a part of the defense system against the stress.

Keywords

Chromium CRED-RA RAPD DNA methylation 

Abbreviations

GTS

Genomic template stability

GA

Gibberellic acid

ZA

Zeatin

IAA

Indole acetic acid

ABA

Absisic acid

ROS

Reactive oxygen species

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2014

Authors and Affiliations

  • Filiz Aygun Erturk
    • 1
  • Guleray Agar
    • 2
  • Esra Arslan
    • 2
  • Gokce Nardemir
    • 3
  • Zehra Sahin
    • 2
  1. 1.Faculty of Science, Department of Molecular BiologyAvrasya UniversityTrabzonTurkey
  2. 2.Laboratory of Molecular Biology, Department of Biology, Faculty of ScienceAtatürk UniversityErzurumTurkey
  3. 3.Department of Molecular Biology and Genetics, Faculty of ScienceAgri Ibrahim Cecen UniversityAgriTurkey

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