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Acta Physiologiae Plantarum

, Volume 35, Issue 9, pp 2721–2726 | Cite as

Effects of different light quality on growth, chlorophyll concentration and chlorophyll biosynthesis precursors of non-heading Chinese cabbage (Brassica campestris L.)

  • XiaoXue Fan
  • Jie Zang
  • ZhiGang Xu
  • ShiRong Guo
  • XueLei Jiao
  • XiaoYing Liu
  • Ying Gao
Original Paper

Abstract

The aim of this study was to evaluate the effects of different light quality of light emitting diode (LED) on the growth, concentration of chlorophyll and chlorophyll biosynthesis precursors of non-heading Chinese cabbage (Brassica campestris L.). Seedlings of the cultivar Te Ai Qing were cultured for 28 days under 6 treatments: red light (R), blue light (B), green light (G), yellow light (Y), red plus blue light (RB) and dysprosium lamp (CK). Lighting experiments were performed under controlled conditions (photon flux density 150 μmol m−2 s−1; 12 h photoperiod; 18–20 °C). The fresh and dry mass were the greatest under RB, which were significantly higher than other light treatments. The fresh mass under RB was almost twice higher compared to other light treatments. Plant height was highest under R treatment and was lowest under B. RB treatment also lowered the plant height significantly. The highest soluble sugar concentration was observed under B. The soluble protein concentration was the greatest under RB. The R treatment was adverse to pigment accumulation. The concentration of photosynthetic pigments and chlorophyll biosynthesis precursors were higher under RB. The RB treatment was beneficial to pigment accumulation.

Keywords

5-Aminolevulinic acid Chlorophyll biosynthesis precursors LEDs Light quality Non-heading Chinese cabbage Photosynthetic pigments 

Abbreviations

ALA

5-Aminolevulinic acid

B

Blue LED

CK

Dysprosium lamp

G

Green LED

LED

Light emitting diode

Mg-Proto IX

Mg-proporphyrin IX

Pchlide

Protochlorophyllide

PFD

Photon flux density

Proto IX

Protoporphyrin IX

R

Red LED

RB

Red plus blue LED

Y

Yellow LED

Notes

Acknowledgments

This research was partially supported by the National Natural Science Foundation of China (30972035), the National 863 High Technology Program of China (2011AA03A114, 2013AA103003), the National Science and Technology Support Project of China (2011BAE01B01), Agricultural research special funds for public welfare projects (201303108), and Jiangsu Science and Technology Key Program (BE2011197). Prof. Feirong Gu of the College of Foreign Studies helped with the language editing of this manuscript.

References

  1. Avercheva OV, Berkovich YA, Erokhin AN, Zhigalova TV, Pogosyan SI, Smolyanina SO (2009) Growth and photosynthesis of Chinese cabbage plants grown under light-emitting diode-based light source. Russ J Plant Physiol 56:14–21CrossRefGoogle Scholar
  2. Britz SJ, Sager JS (1990) Photomorphogenesis and photoassimilation in soybean and sorghum grown under broad spectrum or blue-deficient light sources. Plant Physiol 125:448–454CrossRefGoogle Scholar
  3. Chang TT, Liu XY, Xu ZG, Yang Y (2010) Effects of light spectral energy distribution on growth and development of tomato seedlings. Scientia Agric Sinica 43:1748–1756 (in Chinese)Google Scholar
  4. Dei M (1985) Benzyladenine-induced stimulation of 5-aminolevulinic acid accumulation under various light intensities in levulinic acid-treated cotyledons of etiolated cucumber. Physiol Plantarum 6:153–160CrossRefGoogle Scholar
  5. Fairbairn NJ (1953) A modified anthrone reagent. Chem Ind 31:86Google Scholar
  6. Folta KM, Maruhnich SA (2007) Green light: a signal to slow down or stop. J Exp Bot 58:3099–3111PubMedCrossRefGoogle Scholar
  7. Fukuda N, Fujitan M, Ohta Y, Sase S, Nishimura S, Ezura H (2008) Directional blue light irradiation triggers epidermal cell elongation of abaxial side resulting in inhibition of leaf epinasty in geranium under red light condition. Sci Hortic 115:176–182CrossRefGoogle Scholar
  8. Heo JW, Shin KS, Kim SK, Paek KY (2006) Light quality affect sin vitro growth of grape ‘Teleki 5BB’. J Plant Biol 49:276–280CrossRefGoogle Scholar
  9. Hogewoning SW, Trouwborst G, Maljaars H, Poorter H, Ieperen WV, Harbinson J (2010) Blue light dose-responses of leaf photosynthesis, morphology, and chemical composition of Cucumis sativus grown under different combinations of red and blue light. J Exp Bot 61:3107–3117PubMedCrossRefGoogle Scholar
  10. Holm G (1954) Chlorophyll mutation in barley. Acta Agric Scandinavica 1:457–471CrossRefGoogle Scholar
  11. Hoober JK, Eggink LL (1999) Assembly of light-harvesting complex II and biogenesis of thylakoid membranes in chloroplasts. Photosynth Res 61:197–215CrossRefGoogle Scholar
  12. Jilani A, Kar S, Bose S, Tripathy BC (1996) Regulation of the carotenoid content and chloroplast development by levulinic acid. Physiol Plantarum 96:139–145CrossRefGoogle Scholar
  13. Kamiya A, Ikegami I, Hase E (1981) Effects of light on chlorophyll formation in cultured tobacco cells I. chlorophyll accumulation and phototransformation of protochlorophyll(ide) in callus cells under blue and red light. Plant Cell Physiol 8:1385–1396Google Scholar
  14. Kigel J, Cosgrove DJ (1991) Photoinhibition of stem elongation by blue and red light. Plant Physiol 95:1049–1056PubMedCrossRefGoogle Scholar
  15. Kim SJ, Hahn EJ, Heo JW, Paek KY (2004) Effects of LEDs on net photosynthetic rate, growth and leaf stomata of Chrysanthemum plantlets in vitro. Sci Hortic 101:143–151CrossRefGoogle Scholar
  16. Kurilcik A, Canova MR, Dapkuniene S, Zilinskaite S, Kurilcik G (2008) In vitro culture of Chrysanthemum plantlets using light-emitting diodes. Cent Eur J Biol 2:161–167CrossRefGoogle Scholar
  17. Lee HJ, Ball MD, Parham R, Rebeiz CA (1992) Enzymic conversion of protoporphyrin IX to Mg-protoporphyrin IX in a subplastidic membrane fraction of cucumber etiochloroplasts. Plant Physiol 3:1131–1140Google Scholar
  18. Li HM, Xu ZG, Tang CM (2010) Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypium hirsutum L.) plantlets in vitro. Plant Cell Tiss Org 103:155–163CrossRefGoogle Scholar
  19. Li HM, Xu ZG, Tang CM (2013) Effects of different light resources on growth of upland cotton (Gossypium hirsutum L.) seedlings. Crop Sci AcceptGoogle Scholar
  20. Liu XY, Guo SR, Xu ZG, Jiao XL, Takafumi T (2011) Regulation of chloroplast ultrastructure, cross-section anatomy of leaves and morphology of stomata of cherry tomato by different light irradiations of LEDs. Hortscience 46:217–221Google Scholar
  21. Marsac NT, Houmard J (1993) Adaptation of cyanobacteria to environmental stimuli: new steps towards molecular mechanisms. FEMS Microbiol Rev 1:119–189Google Scholar
  22. Neff MM, Fankhauser C, Chory J (2000) Light: an indicator of time and place. Genes Dev 14:257–271PubMedGoogle Scholar
  23. Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2003) Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell Tiss Org Cult 73:43–52CrossRefGoogle Scholar
  24. Porra RJ (1997) Recent process in porphyrin and chlorophyll biosynthesis. Photochem Photobiol B 65:492–516CrossRefGoogle Scholar
  25. Poudel PR, Kataoka I, Mochioka R (2008) Effect of red-and blue-light-emitting diodes on growth and morphogenesis of grapes. Plant Cell Tiss Org 2:147–153CrossRefGoogle Scholar
  26. Rebeiz CA, Mattheis JR, Smith BB, Rebeiz CC, Dayton DF (1975) Chloroplast biogenesis and accumulation of protochlorophyll by isolaced etioplasts and developing chloroplasts. Arch Biochem Biophys 171:549–567PubMedCrossRefGoogle Scholar
  27. Rivkin RB (1989) Influence of irradiance and spectral quality on the carbon metabolism of phytoplankton. I. Photosynthesis, chemical composition and growth. Mar Ecol Prog Ser 55:291–304CrossRefGoogle Scholar
  28. Saebo A, Krekling T, Appelgren M (1995) Light quality affects photosynthesis and leaf anatomy of birch plantlets in vitro. Plant Cell Tiss Org Cult 41:177–185CrossRefGoogle Scholar
  29. Shin KS, Murthy HN, Heo JW, Hahn EJ, Paek KY (2008) The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants. Acta Physiol Plant 30:339–343CrossRefGoogle Scholar
  30. Sood S, Gupta V, Tripathy BC (2005) Photoregulation of the greening process of wheat seedlings grown in red light. Plant Mol Biol 59:269–287PubMedCrossRefGoogle Scholar
  31. Stobart AK, Griffiths WT, Bukhari AI, Sherwood RP (1985) The effect of Cd2+ on the biosynthesis of chlorophyll in leaves of barley. Physiol Plantarum 3:293–298CrossRefGoogle Scholar
  32. Tanaka R, Tanaka A (2007) Tetrapyrrole biosynthesis in higher plants. Plant Biol 58:321–346CrossRefGoogle Scholar
  33. Tanaka A, Ito H, Tanaka R, Tanaka NK, Yoshida K, Okada K (1998) Chlorophyll a oxygenase (CAO) is involved in chlorophyll b formation from chlorophyll a. Proc Natl Acad Sci USA 21:12719–12723CrossRefGoogle Scholar
  34. Terashima I, Fujita T, Inoue T, Chow WS, Oguchi R (2009) Green light drives leaf photosynthesis more efficiently than red light in strong white light: revisiting the enigmatic question of why leaves are green. Plant Cell Physiol 50:684–697PubMedCrossRefGoogle Scholar
  35. Wang H, Gu M, Cui JX, Shi K (2009) Effects of light quality on CO2 assimilation, chlorophyll-fluorescence quenching, expression of Calvin cycle genes and carbohydrate accumulation in Cucumis sativus. J Photochem Photobiol B 96:30–37PubMedCrossRefGoogle Scholar
  36. Went FW (1957) The experimental control of plant growth. Chronica Botanica Co, Waltham Mass, p 343Google Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2013

Authors and Affiliations

  1. 1.College of Horticulture, Nanjing Agricultural UniversityNanjingChina
  2. 2.College of Agronomy, Nanjing Agricultural UniversityNanjingChina

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