Acta Physiologiae Plantarum

, Volume 35, Issue 8, pp 2407–2419 | Cite as

Characterization and gene mapping of a brittle culm mutant of diploid wheat (Triticum monococcum L.) with irregular xylem vessels development

  • Mohammad Javed Ansari
  • Ahmad Al-Ghamdi
  • Salma Usmani
  • Rahul Kumar
  • Adgaba Nuru
  • Kuldeep Singh
  • Harcharan Singh Dhaliwal
Original Paper
First Online:
Received:
Revised:
Accepted:

Abstract

Diploid wheat, Triticum monococcum L. (einkorn) is an ideal plant material for wheat functional genomics. Brittle culm mutant was identified by screening of the ethyl methane sulphonate-treated M2 progenies of a T. monococcum accession pau14087 by banding the plant parts manually. The brittle culm mutant with drooping leaves, early flowering, reduced tiller numbers and susceptible to lodging had also exhibited brittleness in all plant parts than the wild-type parents. Comprehensive mechanical strength, histological, biochemical, scanning electron microscopy, and Fourier transform infrared spectroscopy analyses of brittle culm mutant supplemented and complemented the findings that the mutant had defective cellulose biosynthesis pathway and deposition of cell wall materials on secondary cell wall of mechanical tissues. Microscopic studies demonstrated that the decrease in cellulose contents resulted in the irregular cell wall organization in xylem vessels of leaf vascular bundles. To map the brc5 mutant, mapping populations were developed by crossing the brittle culm mutant with wild Triticum boeoticum acc. pau5088, having non-brittle characters. The brittle culm mutation was mapped between SSR markers, Xcfd39 and Xgwm126 on 5AmL chromosome of T. monococcum, with genetic distances of 2.6 and 4.8 cM, respectively. The brc5 mutant mapped on 5AmL, being distinct from a previously mapped brittle culm mutant in wheat, has been designated as brc5. The work on fine mapping and map-based cloning of brc5 gene regulating synthesis and deposition of cellulose on the secondary cell wall is in progress.

Keywords

brc5 mutant Cellulose Gene mapping Irregular xylem vessels SSR marker Triticum monococcum (L.) 

Abbreviations

BSA

Bulk segregant analysis

brc5

Brittle culm mutant 5

EMS

Ethyl methane sulfonate

IIT

Indian Institute of Technology

M2

Second generation after mutagenesis

F2

Second filial generation

PCR

Polymerase chain reaction

RILs

Recombinant inbred lines

SEM

Scanning electron microscopy

FTIR

Fourier transform infrared spectroscopy

VBs

Vascular bundles

TS

Transverse section

WT

Wild-type

Notes

Acknowledgments

The authors are grateful to Deanship of Scientific Research and College of Food and Agriculture Science Research, King Saud University Riyadh for providing research support.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2013

Authors and Affiliations

  • Mohammad Javed Ansari
    • 1
    • 6
  • Ahmad Al-Ghamdi
    • 1
  • Salma Usmani
    • 3
  • Rahul Kumar
    • 2
  • Adgaba Nuru
    • 1
  • Kuldeep Singh
    • 4
  • Harcharan Singh Dhaliwal
    • 5
    • 6
  1. 1.Department of Plant Protection, College of Food and Agriculture SciencesKing Saud UniversityRiyadhKingdom of Saudi Arabia
  2. 2.D.K.M. College for WomenThiruvalluvar UniversityVelloreIndia
  3. 3.Department of BiotechnologyICFAI UniversityDehradunIndia
  4. 4.School of Agricultural BiotechnologyPunjab Agricultural UniversityLudhianaIndia
  5. 5.Akal School of BiotechnologyEternal UniversityBaru SahibIndia
  6. 6.Department of BiotechnologyIndian Institute of TechnologyRoorkeeIndia

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