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Acta Physiologiae Plantarum

, Volume 35, Issue 7, pp 2205–2217 | Cite as

Root restriction affected anthocyanin composition and up-regulated the transcription of their biosynthetic genes during berry development in ‘Summer Black’ grape

  • Bo Wang
  • Jianjun He
  • Yang Bai
  • Xiuming Yu
  • Jiefa Li
  • Caixi Zhang
  • Wenping Xu
  • Xianjin Bai
  • Xiongjun Cao
  • Shiping Wang
Original Paper

Abstract

Root restriction was applied to ‘Summer black’ grape (Vitis vinifera L. × Vitis labrusca L.) to investigate its effect on anthocyanin biosynthesis in grape berry during development. Anthocyanin composition and expression patterns of 16 genes in anthocyanin pathway were thus analyzed. The results showed that the anthocyanin levels in berry skin were significantly increased and the anthocyanin profile was enriched. Gene expression pattern revealed that the increased anthocyanins coincide with the up-regulated expression of all 16 genes investigated, including phenylalanine ammonia-lyase, 4-coumarate CoA ligase, chalcone synthase 1, chalcone synthase 2, chalcone synthase 3, chalcone isomerase, flavanone 3-hydroxylase 1, flavanone 3-hydroxylase 2, flavonoid 3′-hydroxylase (F3′H), flavonoid 3′,5′-hydroxylase (F3′5′H), di-hydroflavonol 4-reductase, leucoanthocyanidin dioxygenase, O-methyltransferases (OMT), UDP-glucose:flavonoid 3-O-glucosyl-transferase (3GT), UDP-glucose:flavonoid 5-O-glucosyl-transferase (5GT) and glutathione S-transferase (GST). The increased total anthocyanins predominantly resulted from the increase of tri-hydroxylated, methoxylated and mono-glycosylated rather than di-hydroxylated, non-methoxylated, and di-glycosylated forms, which might be due to the differential regulation of F3′5′H/F3′H, OMT and 3GT, respectively.

Keywords

Vitis vinifera L. × Vitis labrusca L. Root restriction Anthocyanins Gene expression Composition Content 

Abbreviation

TSS

Total soluble solid

TA

Titratable acid

Cy

Cyanidin

Pn

Peonidin

Dp

Delphinidin

Pt

Petunidin

Mv

Malvidin

Pg

Pelargonidin

PAL

Phenylalanine ammonia-lyase

C4H

Cinnamate-4-hydroxylase

4CL

4-Coumarate CoA ligase

CHS

Chalcone synthase

CHI

Chalcone isomerase

F3H

Flavanone 3β-hydroxylase

F3′H

Flavonoid 3′-hydroxylase

F3′5′H

Flavonoid 3′,5′-hydroxylase

DFR

Di-hydroflavonol 4-reductase

LDOX

Leucoanthocyanidin dioxygenase

OMT

O-Methyltransferase

3GT

UDP-Glucose:flavonoid 3-O-glucosyltransferase

5GT

UDP-Glucose:flavonoid 5-O-glucosyltransferase

ACT

Anthocyanin acyltransferase

Glu

Glucoside

GST

Glutathione S-transferase

DAA

Days after anthesis

Notes

Acknowledgments

This research was supported by the Special Funds of Modern Industrial Technology System for Agriculture (CARS-30) and National Natural Science Foundation of China (31201580/C150101). We wish to thank Dr. Juan Xu from National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, for her comments and corrections.

Supplementary material

11738_2013_1257_MOESM1_ESM.doc (36 kb)
Supplementary Table S1 (DOC 35 kb)

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2013

Authors and Affiliations

  • Bo Wang
    • 1
  • Jianjun He
    • 1
  • Yang Bai
    • 2
  • Xiuming Yu
    • 1
  • Jiefa Li
    • 1
  • Caixi Zhang
    • 1
  • Wenping Xu
    • 1
  • Xianjin Bai
    • 3
  • Xiongjun Cao
    • 4
  • Shiping Wang
    • 1
  1. 1.Department of Plant Science, School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghaiChina
  2. 2.Guangxi Nanning Xiangsi Grape Agriculture and Technology Limited CompanyNanningChina
  3. 3.Guangxi Academy of Agricultural SciencesNanningChina
  4. 4.Grape and Wine Research Institute of Guangxi Agriculture Science AcademyNanningChina

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