Acta Physiologiae Plantarum

, Volume 35, Issue 7, pp 2075–2083 | Cite as

Morphological, physiological, cytological and phytochemical studies in diploid and colchicine-induced tetraploid plants of Echinacea purpurea (L.)

  • Mohammad Abdoli
  • Ahmad MoieniEmail author
  • Hassanali Naghdi Badi
Original Paper


Echinacea purpurea (L.) is one of the important medicinal plant species. To obtain the tetraploid plants of Echinacea purpurea with improved medicinal qualities, the root tips of two true leaves seedlings were imbibed in 0.25 % (w/v) colchicine solution for 24, 48, 72, 96 and 168 h. The ploidy level of plants was determined by chromosome counting of root tip cells, and confirmed by flow cytometric analysis. Tetraploid induction occurred in seedlings treated for 24, 48 and 72 h at colchicine solution. The morphological, physiological, cytological, and phytochemical characteristics of diploid and colchicine-induced tetraploid plants were compared. Results indicated that tetraploid plants had considerable larger stomata, pollen grain, seed and flower. Moreover, chloroplast number in guard cells, amount of chlorophyll (a, b, and a + b), carotenoids as well as width and thickness of leaves were increased in tetraploids. However, stomata frequency, leaf index, plant height, and quantum efficiency of photosystem II in tetraploid were lower than diploid plants. High-performance liquid chromatography analysis showed that leaves of the tetraploid plants had more cichoric acid (45 %) and chlorogenic acid (71 %) than diploid plants. It was concluded that morphological and physiological characteristics can be used as useful parameters for preliminary screening of putative tetraploids in this species.


Chromosome counting Colchicine Echinacea purpurea Flow cytometry Physiological characteristics Tetraploid 



Caffeic acid derivatives




Dry weight


Flow cytometry


High-performance liquid chromatography


Murashige and Skoog


Standard error


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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2013

Authors and Affiliations

  • Mohammad Abdoli
    • 1
  • Ahmad Moieni
    • 1
    Email author
  • Hassanali Naghdi Badi
    • 2
  1. 1.Faculty of Agriculture, Department of Plant Breeding and BiotechnologyUniversity of Tarbiat ModaresTehranIran
  2. 2.Cultivation and Development Department of Medicinal Plants Research CenterInstitute of Medicinal Plants, ACECRKarajIran

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