Advertisement

Acta Physiologiae Plantarum

, Volume 35, Issue 5, pp 1675–1689 | Cite as

Development of a Polygonum minus cell suspension culture system and analysis of secondary metabolites enhanced by elicitation

  • Muhammad Faizan A. Shukor
  • Ismanizan Ismail
  • Zamri Zainal
  • Normah Mohd Noor
Original Paper

Abstract

Polygonum minus has been reported to contain valuable metabolites and to date, there is no report on using cell culture technique for metabolite production in P. minus. Naphthalene acetic acid (NAA) concentrations in the range of 2–6 mg L−1 were used in a matrix of combinations with dichlorophenoxyacetic acid (2,4-D) concentrations in the range of 2–10 mg L−1 as plant growth regulators (PGRs) to induce callus cultures. Media that were supplemented with 2 mg L−1 2,4-D + 4 mg L−1 NAA, 2 mg L−1 2,4-D + 6 mg L−1 NAA and 6 mg L−1 2,4-D + 8 mg L−1 NAA were effective for callus induction (93.3 % of the explants produced callus). To establish cell culture, the best growth was obtained from medium that was supplemented with 1 mg L−1 2,4-D + 2 mg L−1 NAA. From a 1-g inoculum size, the fresh weight increases exponentially after 5–10 days of culture, and a 26.71 g maximum fresh weight was obtained after 25 days of culture. The cell culture medium was then analyzed using gas chromatography–mass spectrometry (GC–MS). Jasmonic acid (100, 50, 25 and 5 μM), salicylic acid (100, 50, 25 and 5 μM), yeast extract (500, 250 and 100 mg L−1) and glass beads were used in this research as elicitors. The cell cultures were then incubated with the different elicitors for 1, 2, 3 and 4 days. Several compounds with high peak area percentages were detected, including 2-furancarboxaldehyde, 5-hydroxymethyl, furfural, and 2-cyclopenten-1-one, 2-hydroxy. These results show the diversity of metabolites released by P. minus cell into the culture medium under control conditions.

Keywords

Suspension culture Secondary metabolite Elicitation Polygonum minus 

Notes

Acknowledgments

We would like to thank the Universiti Kebangsaan Malaysia for financing the research project under the Research University grant (UKM-AP-KPB-17-2010).

References

  1. Abdulmalik O, Safo MK, Chen Q, Yang J, Brugnara C, Ohene-Frempong K, Abraham DJ, Asakura T (2005) 5-hydroxymethyl-2-furfural modifies intracellular sickle haemoglobin and inhibits sickling of red blood cells. Br J Haematol 128:552–561PubMedCrossRefGoogle Scholar
  2. Almagro L, Perez AJL, Pedreno MA (2011) New method to enhance ajmalicine production in Catharanthus roseus cell cultures based on the use of cyclodextrins. Biotechnol Lett 33:381–385PubMedCrossRefGoogle Scholar
  3. Atanas P, Simeon P, Elena K, Milen G, Mladenka I (2005) Volatile and polar compounds in Rosa damascene Mill 1803 cell suspension. J Biotechnol 118:89–97CrossRefGoogle Scholar
  4. Baharum SN, Bunawan H, Abd Ghani M, Mustapha WAW, Noor MN (2010) Analysis of the chemical composition of the essential oil of Polygonum minus Huds. using two-dimensional gas chromatography–time-of-flight mass spectrometry (GC–TOF MS). Molecules 15:7006–7015Google Scholar
  5. Blando F, Scardino AP, De Bellis L, Nicoletti I, Giovinazzo G (2005) Characterization of in vitro anthocyanin-producing sour cherry (Prunus cerasus L.) callus cultures. Food Res Int 38:937–942CrossRefGoogle Scholar
  6. Buchbauer G, Jirovetz L, Wasicky M, Nikiforov A (1994) Volatiles of common horsechest nut (Aesculus hippocastanum L.) (Hippocastanaceae) blossoms. J Essent Oil Res 6:93–95CrossRefGoogle Scholar
  7. Chetana R, Ramawat KG (2009) Elicitor induced accumulation of stilbenes in cell suspension cultures of Cayratia trifolia (L.) Domin. Plant Biotechnol Rep 3:135–138CrossRefGoogle Scholar
  8. Claudio DO, Agnieszka C, Christopher D, Paul KB (2009) Induction of secondary metabolism in grape cell cultures by jasmonates. Funct Plant Biol 36:323–338CrossRefGoogle Scholar
  9. Condori J, Sivakumar G, Hubstenberger J, Dolan MC, Sobolev VS, Medina-Bolivar F (2010) Induced biosynthesis of resveratrol and the prenylated stilbenoids arachidin-1 and arachidin-3 in hairy root cultures of peanut: effects of culture medium and growth stage. Plant Physiol Biochem 48:310–318PubMedCrossRefGoogle Scholar
  10. Creelman RA, Mullet JE (1997) Biosynthesis and action of jasmonates in plants. Annu Rev Plant Physiol Plant Mol Biol 48:355–382PubMedCrossRefGoogle Scholar
  11. Da Silva UF, Borba EL, Semir J, Marsaioli AJ (1999) A simple solid injection device for the analyses of Bulbophyllum (Orchidaceae) volatiles. Phytochemistry 50:31–34CrossRefGoogle Scholar
  12. Dalluge J, Beens J, Brinkman UAT (2003) Comprehensive two dimensional gas chromatography, a powerful and versatile analytical tool. J Chromatogr A 1000:69–108PubMedCrossRefGoogle Scholar
  13. Dicosmo F, Misawa M (1995) Plant cells and tissue culture, alternatives for metabolite production. Biotechnol Adv 13:425–453PubMedCrossRefGoogle Scholar
  14. Donenburg H, Knorr D (1995) Strategies for the improvement of secondary metabolite production in plant cell cultures. Enzyme Microb Technol 17:674–684CrossRefGoogle Scholar
  15. Durner J, Shah J, Klessig DF (1997) Salicylic acid and disease resistance in plants. Trends Plant Sci 2:266–274CrossRefGoogle Scholar
  16. Elena ES, Beatriz EJ, Jairo RM (2004) Comparison of different extraction methods for the analysisof volatile secondary metabolites of Lippia alba (Mill.) N.E. Brown, grown in Colombia, and evaluation of its in vitro antioxidant activity. J Chromatogr A 1025:93–103CrossRefGoogle Scholar
  17. Estrada ZME, Cruz-Sosa F, Rodrıguez-Monroy M, Verde-Calvo JR, Vernon-Carter EJ (2009) Phenylpropanoid production in callus and cell suspension cultures of Buddleja cordata Kunth. Plant Cell Tissue Organ Cult 97:39–47CrossRefGoogle Scholar
  18. Fan GQ, Zhai XQ, Zhai CJ, Bi HT (2001) Callus induction from leaves of different Paulownia species and its plantlet regeneration. J For Res 12:209–214CrossRefGoogle Scholar
  19. Farmer EE, Ryan CA (1990) Interplant comunication: airbone methyl jasmonate induces synthesis of proteinase inhibitor in plant leaves. Proc Natl Acad Sci 87:7713–7716PubMedCrossRefGoogle Scholar
  20. Fernandes ES, Passos GF, Medeiros R, da Cunha FM, Ferreira J, Campos MM, Pianowski LF, Calixto JB (2007) Anti-inflammatory effects of compounds alpha-humulene and (−)-trans-caryophyllene isolated from the essential oil of Cordia verbenacea. Eur J Pharmacol 569:228–236PubMedCrossRefGoogle Scholar
  21. Ferri M, Dipalo SCF, Bagni N, Tassoni A (2011) Chitosan elicits mono-glucosylated stilbene production and release in fed-batch bioreactor cultures of grape cells. Food Chem 124:1473–1479CrossRefGoogle Scholar
  22. Gunjan P, Ashok KS (2008) Statistical elicitor optimization studies for the enhancement of azadirachtin production in bioreactor Azadirachta indica cell cultivation. Biochem Eng J 40:218–226CrossRefGoogle Scholar
  23. Huber GW, Iborra S, Corma A (2006) Synthesis of transportation fuels from biomass: chemistry, catalysts, and engineering. Chem Rev 106:4044–4098PubMedCrossRefGoogle Scholar
  24. Huda-Faujan N, Noriham A, Norrakiah AS, Babji AS (2007) Antioxidative activities of water extracts of some Malaysian herbs. ASEAN Food J 14:61–68Google Scholar
  25. Hui C, Feng C (2000) Effects of yeast elicitor on the growth and secondary metabolism of a high-tanshinone-producing line of the Ti transformed Salvia miltiorrhiza cells in suspension culture. Process Biochem 35:837–840CrossRefGoogle Scholar
  26. Joo JH, Jetten AM (2009) Molecular mechanisms involved in farnesol-induced apoptosis. Cancer Lett 287:123–135PubMedCrossRefGoogle Scholar
  27. Jose L, Jose JSS (1999) Molecular biology of jasmonic acid biosynthesis in plants. Plant Physiol Biochem 37:373–380CrossRefGoogle Scholar
  28. Kajani AA, Mofid MR, Abolfazli K, Tafreshi SAH (2010) Encapsulated activated charcoal as a potent agent for improving taxane synthesis and recovery from cultures. Biotechnol Appl Biochem 56:71–76PubMedCrossRefGoogle Scholar
  29. Kang SM, Min JY, Kim YD, Karigar CS, Kim SW, Goo GH, Choi MS (2009) Effect of biotic elicitors on the accumulation of bilobalide and ginkgolides in Ginkgo biloba cell cultures. J Biotechnol 139:84–88PubMedCrossRefGoogle Scholar
  30. Keen NT (1992) The molecular biology of disease reistance. Plant Mol Biol 19(1):109–122PubMedCrossRefGoogle Scholar
  31. Kromidas L, Perrier E, Flanagan J, Rivero R, Bonnet I (2006) Release of antimicrobial actives from microcapsules by the action of axillary bacteria. Int J Cosmet Sci 28:103–108PubMedCrossRefGoogle Scholar
  32. Lee A, Cho K, Jang S, Randeep R, Hitoshi I, Ganesh KA, Jaehan S, Oksoo H (2004) Inverse correlation between jasmonic acid and salicylic acid during early wound response in rice. Biochem Biophys Res Comm 318:734–738PubMedCrossRefGoogle Scholar
  33. Lo KY, Nadali BJ, Chan LK (2012) Investigation on the effect of subculture frequency and inoculum size on the artemisinin content in a cell suspension culture of Artemisia annua L. Aust J Crop Sci 6(5):801–807Google Scholar
  34. Lucia CV, Consuelo DM, Emilia G, Soledad PC (2006) Analysis of volatile compounds of eucalyptus honey by solid phase extraction followed by gas chromatography coupled to mass spectrometry. Eur Food Res Technol 224:27–31CrossRefGoogle Scholar
  35. Mitra, S (ed) (2003) Chapter 2: Principles of extraction. Sample preparation techniques in analytical chemistry. Wiley, New YorkGoogle Scholar
  36. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  37. Nadia P, Izabela KI, Shalini J, Kylie M, Tracey W, Philip F (2003) Effect of methyl jasmonate and p-coumaric acid on anthocyanin composition in a sweet potato cell suspension culture. Biochem Eng J 14:171–177CrossRefGoogle Scholar
  38. Neumann KH (2009) Plant cell and tissue culture—a tool in biotechnology. 181 Principles and Practice. Springer, BerlinGoogle Scholar
  39. Nurazah Z, Radzali M, Syahida A, Maziah M (2009) Effects of plant growth regulators on callus induction from Cananga odorata flower petal explants. Afr J Biotechnol 8:2740–2743Google Scholar
  40. Orban N, Imre B, Zoltan S, Bela D (2008) Influence of different elicitors on the synthesis of anthraquinone derivatives in Rubia tinctorum L. cell suspension cultures. Dyes Pigments 77:249–257CrossRefGoogle Scholar
  41. Pezzuto JM (1995) Natural product cancer chemoprotective agents. In: Arnason JT, Mata R, Romeo JT (eds) Recent advances in phytochemistry. Phytochemistry of medicinal plants, vol 29. Plenum, New York, pp 19–45Google Scholar
  42. Ransika DA, Koki F, Tatsuya A, Kenichi K (2009) Volatile and non volatile monoterpenes produced by elicitor-stimulated Cupressus lusitanica cultured cells. J Plant Physiol 166:720–728CrossRefGoogle Scholar
  43. Rao RS, Ravishankar GA (2002) Plant cell cultures: chemical factories for secondary metabolites. Biotechnol Adv 20:101–153PubMedGoogle Scholar
  44. Raskin I, Skubatz H, Tang W, Meeuse BJD (1990) Salicylic acid levels in thermogenic and non-thermogenic plants. Ann Bot 66:369–373Google Scholar
  45. Ravishankar GA, Venkataraman LV (1993) Role of plant cell culture in food biotechnology: current trends, limitations and future prospects. In: Prakash J, Pierik RLM (eds) Plant biotechnology: commercial prospects and problems. Oxford IBH Press, New Delhi, pp 255–274Google Scholar
  46. Reymond P, Farmer EE (1998) Jasmonate and salicylate as global signals for defense gene expression. Curr Opin Plant Biol 1:404–411PubMedCrossRefGoogle Scholar
  47. Sahai O (1994) Plant tissue culture. In: Gabelman A (ed) Bioprocess production of flavor, fragrance, and color ingredients. Wiley, NewYork, pp 239–275Google Scholar
  48. Sampedro MAS, Jorge FT (2005) Yeast extract and methyl jasmonate-induced silymarin production in cell cultures of Silybum marianum (L.) Gaertn. J Biotechnol 119:60–69CrossRefGoogle Scholar
  49. Shinjiro O (2005) Callus and cell suspension culture of bamboo plant, Phyllostachys nigra. Plant Biotechnol 22:119–125CrossRefGoogle Scholar
  50. Shobana S, Vidhya VG, Ramya M (2009) Antibacterial activity of garlic varieties (Ophioscordon and Sativum) on enteric pathogens. Curr Res J Biol Sci 1:123–126Google Scholar
  51. Shulaev V, Leon J, Raskin I (1995) Is salicylic acid a translocated signal of systemic acquired resistance in tobacco? Plant Cell 7:1691–1701PubMedGoogle Scholar
  52. Sikirat RA, Morufat OB, Benjamin AO (2009) Effects of plant growth regulators and explant types on callus formation in African yam bean (Sphenostylis stenocarpa (Hochst. Ex A. Rich) Harms). Kasetsart J (Nat Sci) 43:442–448Google Scholar
  53. Travis SW, Harsh PB, Jorge MV (2002) Jasmonic acid-induced hypericin production in cell suspension cultures of Hypericum perforatum L. (St. John’s wort). Phytochemistry 60:289–293CrossRefGoogle Scholar
  54. Tyagi PL, Comai Byers B (2001) Comparison of plant regeneration from root, shoot and leaf explants in pigeon pea (Cajanus cajan) cultivars. SABRAO J Breed Genet 33:59–71Google Scholar
  55. Tzer MC, Mohd AA, Oi ML, Fadzillah M, Nor’AiniNordin HL (2005) Effective elicitation factors in Morinda elliptica cell suspension culture. Process Biochem 40:3397–3405CrossRefGoogle Scholar
  56. Umer R, Shaukat A, Ghulam MA, Najma A, Shahid MM (2009) Establishment of an efficient callus induction and plant regeneration system in Pakistani wheat (Triticum aestivum) cultivars. Electron J Biotechnol. doi: 10.2225/vol12-issue3-fulltext-1 Google Scholar
  57. Vanisree M, Hsin ST (2004) Plant cell cultures—an alternative and efficient source for the production of biologically important secondary metabolites. Int J Appl Sci Eng 2:29–48Google Scholar
  58. Wan Hassan WE (2007) Healing Herbs of Malaysia. Cetakulang Federal Land Development Authority (FELDA), Kuala LumpurGoogle Scholar
  59. Wasternack C, Stenzel I, Hause B, Kutter C, Maucher H, Neumerkel J, Feussner I, Miersch O (2006) The wound response in tomato-role of jasmonic acid. J Plant Physiol 163(3):297–306PubMedCrossRefGoogle Scholar
  60. Weichert H, Stenzel I, Berndt E, Wasternack C, Feussner I (1999) Metabolic profiling of oxylipins upon salicylate treatment in barley leaves—preferential induction of the reductase pathway by salicylate. FEBS Lett 464:133–137PubMedCrossRefGoogle Scholar
  61. Wink M (1994) The cell-culture medium—a functional extracellular compartment of suspension-cultured cells. Plant Cell Tissue Organ Cult 38:307–319CrossRefGoogle Scholar
  62. Xu M, Yang B, Dong J, Lu D, Jin H, Sun L, Zhu Y, Xu X (2011) Enhancing hypericin production of Hypericum perforatum cell suspension culture by ozone exposure. Biotechnol Prog 27:1101–1106PubMedCrossRefGoogle Scholar
  63. Yaakob BK (1987) Kesom oil—a natural source of aliphatic aldehydes. Perfum Flavour 12:27–30Google Scholar
  64. Yang ZS, Chen GD, Li YX, Chen J (2009) Characterization of callus formation in leaf of Euphorbia helioscopia. Afr J Plant Sci 3:122–126Google Scholar
  65. Yanpaisan W, King NJC, Doran PM (1999) Flow cytometry of plant cells with applications in large-scale bioprocessing. Biotechnol Adv 17:3–27PubMedCrossRefGoogle Scholar
  66. Yu LJ, Lan WZ, Qin WN, Xu HB (2001) Effects of salicylic acid on fungal elicitor-induced membrane-lipid peroxidation and taxol production in cell suspension cultures of Taxus chinensis. Process Biochem 37:477–482CrossRefGoogle Scholar
  67. Zamboni A, Gatto P, Cestaro A, Pilati S, Viola R, Mattivi F, Moser C, Velasco R (2009) Grapevine cell early activation of specific responses to DIMEB, a resveratrol elicitor. BMC Genomics 10:363. doi: 10.1186/1471-2164-10-363 PubMedCrossRefGoogle Scholar
  68. Zhang J, Guo Z (2006) Effect of methyl jasmonic acid on baccatin III biosynthesis. Tsinghua Sci Technol 11:363–367CrossRefGoogle Scholar
  69. Zhang CH, Fevereiro PS, He G, Chen Z (2007) Enhanced paclitaxel productivity and release capacity of Taxus chinensis cell suspension cultures adapted to chitosan. Plant Sci 172:158–163CrossRefGoogle Scholar
  70. Zhao JT, Lawrence CD, Robert V (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283–333PubMedCrossRefGoogle Scholar
  71. Zhenzhen C, Iryna S, Anja K, Dietrich K (2012) Exudation: an expanding technique for continuous production and release of secondary metabolites from plant cell suspension and hairy root cultures. Plant Cell Rep 31:461–477CrossRefGoogle Scholar
  72. Zhu LW, Zhong JJ, Tang YJ (2008) Significance of fungal elicitors on the production of ganoderic acid and Ganoderma polysaccharides by the submerged culture of medicinal mushroom Ganoderma lucidum. Process Biochem 43:1359–1370CrossRefGoogle Scholar
  73. Zi XL, Anna PKL, Sobri H (2009) Callus Induction of Ocimum sanctum and estimation of its total flavonoids content. Asian J Agric Sci 1(2):55–61Google Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2013

Authors and Affiliations

  • Muhammad Faizan A. Shukor
    • 1
    • 2
  • Ismanizan Ismail
    • 1
    • 2
  • Zamri Zainal
    • 1
    • 2
  • Normah Mohd Noor
    • 2
  1. 1.School of Biosciences and Biotechnology, Faculty of Science and TechnologyUniversiti Kebangsaan MalaysiaBangiMalaysia
  2. 2.Institute of System Biology (INBIOSIS)Universiti Kebangsaan MalaysiaBangiMalaysia

Personalised recommendations