Acta Physiologiae Plantarum

, Volume 35, Issue 3, pp 959–967

Drought tolerance of Periploca sepium during seed germination: antioxidant defense and compatible solutes accumulation

Original Paper


Periploca sepium Bunge is a native and widespread shrub on the Loess Plateau, an arid and semi-arid region in China. To understand the adaptability of its seed germination to dry environments, we investigated the germination rate, water relations, lipid peroxidation, antioxidant capacity and accumulation of major organic solutes during seed germination under water deficit conditions. Results showed that seeds pre-treated by hydration–dehydration or −0.9 MPa PEG germinated faster than control seeds, indicating strong resistance of P. sepium to drought condition. The re-dried seeds showed higher proline, total free amino acids (TFAA) and soluble proteins (SP) contents than control dry seeds, indicating the maintenance of physiological advancement when dehydrated. Osmotic stress made seed germination stay on the plateau phase (phase II). However, germinating seeds moved into phase III immediately once transferred into distilled water. Large increases in SP and soluble sugars (SS) of both re-dried and osmotic stressed seeds help themselves to resist drought stress. The re-hydrated seeds showed significantly higher levels of proline, TFAA, SP and SS than control seeds. The largely accumulated SS during osmotic stress declined sharply when transferred into distilled water. Our data demonstrate that P. sepium’s tolerance to drought stresses during germination is associated with enhanced activity of antioxidant enzymes and accumulation of some compatible solutes. Seed physiological advancement progressed slowly under low water conditions and it was maintained when seeds were air dried. This strategy ensures high and more rapid seed germination of P. sepium under drying and wetting conditions in drought-prone regions.


Germination mechanisms Organic solutes Hydration–dehydration Osmotic stress 


  1. Abrahamsen M, Sudia TM (1966) Studies on the soluble carbohydrates and carbohydrate precursors in germinating soybean seed. Am J Bot 53:108–114CrossRefGoogle Scholar
  2. Ahmadi A, Mardeh AS, Poustini K, Jahromi ME (2007) Influence of osmo and hydropriming on seed germination and seedling growth in wheat (Triticum aestivum L.) cultivars under different moisture and temperature conditions. Pak J Biol Sci 10:4043–4049PubMedCrossRefGoogle Scholar
  3. Amini F, Ehsanpour AA (2005) Soluble proteins, proline, carbohydrates and Na+/K+ changes in two tomato (Lycopersicon esculentum Mill.) cultivars under in vitro salt stress. Am J Biochem Biotechnol 1:212–216Google Scholar
  4. An YY, Liang ZS, Zhang Y (2011a) Seed germination responses of Periploca sepium Bunge, a dominant shrub in the Loess hilly regions of China. J Arid Environ 75:504–508CrossRefGoogle Scholar
  5. An YY, Liang ZS, Zhao RK, Zhang J, Wang XJ (2011b) Organ-dependent responses of Periploca sepium to repeated dehydration and rehydration. S Afr J Bot 77:446–454CrossRefGoogle Scholar
  6. Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399PubMedCrossRefGoogle Scholar
  7. Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59:206–216CrossRefGoogle Scholar
  8. Bartels D, Sunkar R (2005) Drought and salt tolerance in plants. Crit Rev Plant Sci 24:23–58CrossRefGoogle Scholar
  9. Bewley JD (1997) Seed germination and dormancy. Plant Cell 9:1055–1066PubMedCrossRefGoogle Scholar
  10. Blokhina O, Virolainen E, Fagerstedt KV (2003) Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot 91:179–194PubMedCrossRefGoogle Scholar
  11. Bohnert HJ, Shen B (1999) Transformation and compatible solutes. Sci Hortic 78:237–260CrossRefGoogle Scholar
  12. Bradford KJ (1990) A water relations analysis of seed germination rates. Plant Physiol 94:840–849PubMedCrossRefGoogle Scholar
  13. Chaves MM, Pereira JS, Maroco J, Rodrigues ML, Ricardo CPP, Osorio ML, Carvalho I, Faria T, Pinheiro C (2002) How plants cope with water stress in the field. Photosynthesis and growth. Ann Bot 89:907–916PubMedCrossRefGoogle Scholar
  14. Chen ZH, Cuin TA, Zhou MX, Twomey A, Naidu BP, Shabala S (2007) Compatible solute accumulation and stress-mitigating effects in barley genotypes contrasting in their salt tolerance. J Exp Bot 58:4245–4255PubMedCrossRefGoogle Scholar
  15. Ducic T, Liric-Rajlic I, Mitrovic A, Radotic K (2003) Activities of antioxidant systems during germination of Chenopodium rubrum seeds. Biol Plant 47:527–533CrossRefGoogle Scholar
  16. EI-Maarouf-Bouteau H, Bailly C (2008) Oxidative signaling in seed germination and dormancy. Plant Signal Behav 3:175–182CrossRefGoogle Scholar
  17. Fan XW, Li FM, Song L, Xiong YC, An LZ, Jia Y, Fang XW (2009) Defense strategy of old and modern spring wheat varieties during soil drying. Physiol Plant 136:310–323PubMedCrossRefGoogle Scholar
  18. Ghassemi-Golezani K, Aliloo AA, Valizadeh M, Moghaddam M (2008) Effects of hydro and osmo-priming on seed germination and field emergence of lentil (Lens culinaris Medik.). Not Bot Horti Agrobo 36:29–33Google Scholar
  19. Hegarty TW (1977) Seed activation and seed germination under moisture stress. New Phytol 78:349–359CrossRefGoogle Scholar
  20. Ismail AM, Ella ES, Vergara GV, Mackill DJ (2009) Mechanisms associated with tolerance to flooding during germination and early seedling growth in rice (Oryza sativa). Ann Bot 103:197–209PubMedCrossRefGoogle Scholar
  21. Jones HG, Corlett JE (1992) Current topics in drought physiology. J Agr Sci 119:291–296CrossRefGoogle Scholar
  22. Kameli A, Losel DM (1995) Contribution of carbohydrates and other solutes to osmotic adjustment in wheat leaves under water-stress. J Plant Physiol 145:363–366CrossRefGoogle Scholar
  23. Kaya MD, Okcu G, Atak M, Cikili Y, Kolsarici O (2006) Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). Eur J Agron 24:291–295CrossRefGoogle Scholar
  24. Li W, Wang QJ, Wei SP, Shao MA, Yi L (2008) Soil desiccation for Loess soils on natural and regrown areas. Forest Ecol Manag 255:2467–2477CrossRefGoogle Scholar
  25. Mattioli R, Costantino P, Torovato M (2009) Proline accumulation in plants: not only stress. Plant Signal Behav 4:1016–1018PubMedCrossRefGoogle Scholar
  26. Miazek A, Bogdan J, Zagdanska B (2001) Effects of water deficit during germination of wheat seeds. Biol Plant 44:397–403CrossRefGoogle Scholar
  27. Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410PubMedCrossRefGoogle Scholar
  28. Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9:490–498PubMedCrossRefGoogle Scholar
  29. Moradi A, Younesi O (2009) Effects of osmo- and hydro-priming on seed parameters of grain sorghum (Sorghum bicolor L.). Aust J Basic Appl Sci 3:1696–1700Google Scholar
  30. Morgan JM (1984) Osmoregulation and water stress in higher plants. Annu Rev Plant Physiol 35:299–319CrossRefGoogle Scholar
  31. Paul AK, Mukherji S (1972) Change in respiration rate of rice seedlings as affected by storage and viability, and its possible relation with catalase and peroxidase activities during germination. Biol Plant 14:414–419CrossRefGoogle Scholar
  32. Rai VK (2002) Role of amino acids in plant responses to stresses. Biol Plant 45:481–487CrossRefGoogle Scholar
  33. Sharma S, Villamon JG, Verslues PE (2011) Essential role of tissue-specific proline synthesis and catabolism in growth and redox balance at low water potential. Plant Physiol 157:292–304PubMedCrossRefGoogle Scholar
  34. Sidari M, Mallamaci C, Muscolo A (2008) Drought, salinity and heat differently affect seed germination of Pinus pinea. J Forest Res 13:326–330CrossRefGoogle Scholar
  35. Soltani A, Gholipoor A, Zeinali E (2006) Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environ Exp Bot 55:195–200CrossRefGoogle Scholar
  36. Szabados L, Savoure A (2010) Proline: a multifunctional amino acid. Trends Plant Sci 15:89–97PubMedCrossRefGoogle Scholar
  37. Teketay D (1996) Germination ecology of twelve indigenous and eight exotic multipurpose leguminous species from Ethiopia. Forest Ecol Manag 80:209–223CrossRefGoogle Scholar
  38. Tobe K, Zhang LP, Qiu GY, Shimizu H, Omasa K (2001) Characteristics of seed germination in five non-haplotypic Chinese desert shrub species. J Arid Environ 47:191–201CrossRefGoogle Scholar
  39. Verbruggen N, Hermans C (2008) Proline accumulation in plants: a review. Amino Acids 35:753–759PubMedCrossRefGoogle Scholar
  40. Wang ZL, Wang G, Liu XM (1998) Germination strategy of the temperate sandy desert annual chenopod Agriophyllum squarrosum. J Arid Environ 40:69–76CrossRefGoogle Scholar
  41. Yang CH, Wang YY, Zhou ZF, Zhang GC (2006) Response of gas exchange parameters of Periploca sepium Bunge to soil water content in Loess Plateau. Forest Res 19:231–234 (in Chinese)Google Scholar
  42. Zeng YJ, Wang YR, Zhang JM (2010) Is reduced seed germination due to water limitation a special survival strategy used by xerophytes in arid dunes? J Arid Environ 74:508–511CrossRefGoogle Scholar
  43. Zhu ZB, Liang ZS, Han RL (2009) Saikosaponin accumulation and antioxidative protection in drought-stressed Bupleurum chinense DC. Plants. Environ Exp Bot 66:326–333CrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2012

Authors and Affiliations

  1. 1.College of Life SciencesNorthwest A&F UniversityYanglingPeople’s Republic of China
  2. 2.School of Life scienceZhejiang Sci-Tech UniversityHangzhouPeople’s Republic of China

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