Acta Physiologiae Plantarum

, Volume 35, Issue 3, pp 771–779 | Cite as

In vitro propagation and conservation of Indian sarsaparilla, Hemidesmus indicus L. R. Br. through somatic embryogenesis and synthetic seed production

  • Meena K. Cheruvathur
  • Nezia Najeeb
  • T. Dennis Thomas
Original Paper

Abstract

A protocol has been developed for achieving somatic embryogenesis from callus derived from nodal cuttings and production of synthetic seeds in Hemidesmus indicus L. R. Br. a highly traded ethnomedicinal plant. Proembryogenic, friable, light yellowish callus was induced from the basal cut end of the nodal cuttings on Murashige and Skoog (MS) medium supplemented with 3 μM indole-3-butyric acid (IBA). The highest rate of somatic embryogenesis (92 %) was observed when the callus was subcultured on half strength MS medium supplemented with 2 μM IBA. On induction medium somatic embryos were developed up to the torpedo stage. Further elongation and germination of somatic embryos were obtained in MS medium supplemented with 4 μM 6-benzylaminopurine (BA) in combination with 1.5 μM gibberellic acid (GA3). Somatic embryos were collected and suspended in a matrix of MS medium containing sodium alginate (3 % W/V) dropped into 75 mM calcium chloride (CaCl2·2H2O) solution for the production of synthetic seeds and later transferred to MS medium for germination. The synthetic seeds were successfully germinated on medium even after 120 days of storage at 4 °C. The plantlets were eventually transferred to soil with 92 % success.

Keywords

Conservation Ethnomedicinal plant Hemidesmus indicus In vitro propagation Somatic embryos Synthetic seeds 

Abbreviations

BA

6-Benzylaminopurine

2,4-D

2,4-Dichlorophenoxyacetic acid

GA3

Gibberellic acid

IBA

Indole-3-butyric acid

MS

Murashige and Skoog

NAA

Naphthalene acetic acid

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2012

Authors and Affiliations

  • Meena K. Cheruvathur
    • 1
  • Nezia Najeeb
    • 1
  • T. Dennis Thomas
    • 1
  1. 1.Postgraduate and Research Department of BotanySt. Thomas CollegeKottayamIndia

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