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Acta Physiologiae Plantarum

, Volume 35, Issue 2, pp 575–587 | Cite as

Transcriptional profile of the spring freeze response in the leaves of bread wheat (Triticum aestivum L.)

  • Guozhang Kang
  • Gezi Li
  • Wenping Yang
  • Qiaoxia Han
  • Hongzhen Ma
  • Yonghua Wang
  • Jiangping Ren
  • Yunji Zhu
  • Tiancai Guo
Original Paper

Abstract

Measurement of the electrolyte leakage rates in wheat leaves indicated that there was no significant difference in susceptibility to −5 °C spring freeze stress among five bread wheat cultivars at the floret primordium-differentiating stage of spike development. A global transcriptional profile was created using the Affymetrix Wheat GeneChip microarray for one wheat cultivar (Yumai 34) under −5 °C freeze stress. After assaying genes with significant regulation at 1 and 3 days after −5 °C freeze stress, we identified 600 genes that were previously annotated as showing changes in expression of at least than two-fold at one or both of the time points. Among these genes, we further analysed 102 genes whose expression levels changed at least eight-fold after 1 or 3 days of freeze stress. These genes encoded an ice recrystallization protein, cold-related proteins, CBF transcription factors, calcium-dependent protein kinases, Na+/H+ antiporters, aquaporins, and many metabolic enzymes. The results of this study were compared with those of a previous study on the sub-freeze hardening response in wheat and spring freeze stress in wheat and barley. Many genes, including those encoding WCOR413, LEA, glycine-rich RNA-binding protein, ferritin, aquaporin 2, and a pathogen-induced protein, showed similar expression levels in these studies. Spring freeze stress is a complex phenomenon involving physiological mechanisms and multiple genes that had not been previously characterised.

Keywords

cDNA arrays Gene expression Spring freeze stress Triticum aestivum L. 

Notes

Acknowledgments

This study was supported by funds from the National Transgenic Major Project (2009ZX08002-21B), the Special Fund for Agro-scientific Research in the Public Interest (201003002), and the National Basic Research Program of China (2009CB118602).

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

11738_2012_1099_MOESM1_ESM.doc (1.5 mb)
Supplementary material 1 (DOC 1558 kb)

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2012

Authors and Affiliations

  • Guozhang Kang
    • 1
  • Gezi Li
    • 1
  • Wenping Yang
    • 2
  • Qiaoxia Han
    • 1
  • Hongzhen Ma
    • 1
  • Yonghua Wang
    • 1
  • Jiangping Ren
    • 1
  • Yunji Zhu
    • 1
  • Tiancai Guo
    • 1
  1. 1.National Engineering Research Centre for Wheat, Key Laboratory of Physiology, Ecology and Genetic Improvement of Food Crops in Henan ProvinceHenan Agricultural UniversityZhengzhouChina
  2. 2.Department of AgricultureHenan Institute of Science and TechnologyXinxiangChina

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