Advertisement

Acta Physiologiae Plantarum

, Volume 35, Issue 1, pp 119–128 | Cite as

Electrolyte leakage and chlorophyll a fluorescence among castor bean cultivars under induced water deficit

  • Ana Paula de Faria
  • José Pires Lemos-Filho
  • Luzia Valentina Modolo
  • Marcel Giovanni Costa FrançaEmail author
Original Paper

Abstract

We evaluated leaf fragments of three castor bean cultivars after being subjected to water stress. Leaf discs were exposed to polyethylene glycol (PEG-6000) solutions for tissue dehydration at various water potentials. After water-stress imposition, electrolyte leakage and chlorophyll a fluorescence were used jointly on the same leaf fragments cut from the same plant leaf. Furthermore, these two experimental procedures were adapted to unequivocally distinguish cultivars’ responses to water stress. Electrolyte leakage, ion efflux, membrane injury index and maximum quantum yield of photosystem II showed genotypic differences between cultivars. Despite these genotypic differences, the photosystem II electron transport rate was not significantly affected by water stress. The membrane injury shown may have been transient, probably due to a disarrangement in the phospholipid bilayer. The use of the two experimental procedures on the same leaf samples was less time-consuming and allowed for more reliable results. Furthermore, the procedures proved efficient for selection of physiological water-stress tolerance traits and could be employed in other plant experimental models.

Keywords

Water stress PEG Photosynthesis Protoplasmic resistance Ricinus communis 

Notes

Acknowledgments

The authors are grateful to EMBRAPA-Algodão and the Instituto Agronômico de Campinas for kindly supplying castor bean seeds and to J.S. Garcia, C.K.R. Barbosa, A.C. Souza and M.S. Silva for assistance with plant cultivation and the experiments. We also thank Alistair Hayward for the translation and review of the English version of the text. This work was supported by FAPEMIG.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11738_2012_1054_MOESM1_ESM.jpg (343 kb)
Supplementary material 1 (JPEG 343 kb)

References

  1. Anvisa—Agência Nacional de Vigilância Sanitária, Ministério da Saúde (2005) Formulário Nacional. Ministério da Saúde. Brasília, DF, Brasil: Editora ANVISAGoogle Scholar
  2. Azevêdo DMP, Lima EF, Batista FAZ (1997) Recomendações técnicas para o cultivo da mamoneira (Ricinus communis L.) no Brasil. Campina Grande, PB, Brasil: CNPAGoogle Scholar
  3. Bajji M, Kinet JM, Lutts S (2002) The use of electrolyte leakage method for assessing cell membrane stability as water stress tolerance test in durum wheat. Plant Growth Regul 36:61–70CrossRefGoogle Scholar
  4. Bandurska H (2000) Does proline accumulated in leaves of water deficits stressed barley plants confine cell membrane injury? I. Free proline accumulation and membrane injury index in drought and osmotically stressed plants. Acta Physiol Plant 22:409–415CrossRefGoogle Scholar
  5. Dai Z, Edwards GE, Ku MSB (1992) Control of photosynthesis and stomatal conductance in Ricinus communis L. (castor bean) by leaf to air vapor pressure deficit. Plant Physiol 99:1426–1434PubMedCrossRefGoogle Scholar
  6. Ehwald R, Richter E, Schlangstedt M (1984) Solute leakage from isolated parenchyma of Allium cepa and Kalanchoë daigremontiana. J Exp Bot 35(8):1095–1103CrossRefGoogle Scholar
  7. Ferreira GB, Beltrão NEM, Severino LS, Gondim TMS, Pedrosa MB (2006) A cultura da mamona no Cerrado: riscos e oportunidades. Embrapa. http://www.infoteca.cnptia.embrapa.br/handle/doc/276576. Accessed 17 Nov 2011
  8. Germ M, Kreft I, Stibilj V, Urbanc-Berčič O (2007) Combined effects of selenium and drought on photosynthesis and mitochondrial respiration in potato. Plant Physiol Biochem 45:162–167PubMedCrossRefGoogle Scholar
  9. Han B, Kermode AR (1996) Dehydrin-like proteins in castor bean seeds and seedlings are differentially produced in response to ABA and water-deficit-related stresses. J Exp Bot 47(300):933–939CrossRefGoogle Scholar
  10. Heckenberger U, Roggatz U, Schurr U (1998) Effect of drought stress on the cytological status in Ricinus communis. J Exp Bot 49(319):181–189Google Scholar
  11. Hoagland DR, Arnon DI (1950) The water culture method for growing plants without soils. California Agricultural Experimental Station, BerkeleyGoogle Scholar
  12. Kocheva KV, Lambrev P, Georgiev GI, Goltsev V, Karabaliev M (2004) Evaluation of chlorophyll fluorescence and membrane injury in the leaves of barley cultivars under osmotic stress. Bioelectrochemistry 63:121–124PubMedCrossRefGoogle Scholar
  13. Lauriano JA, Lidon FC, Carvalho CA, Campos PS, Matos MC (2000) Drought effects on membrane lipids and photosynthetic activity in different peanuts cultivars. Photosynthetica 38:7–12CrossRefGoogle Scholar
  14. Levitt J (1980) Responses of plants to environmental stresses. In: Water, radiation, salt and other stresses, vol II. Academic Press, New YorkGoogle Scholar
  15. Li G, Wan S, Zhou J, Yang Z, Qin P (2010) Leaf chlorophyll fluorescence, hyperspectral reflectance, pigments content malondialdehyde and proline accumulation responses of castor bean (Ricinus communis L.) seedlings to salt stress levels. Ind Crop Prod 31:13–19CrossRefGoogle Scholar
  16. Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 603:591–593Google Scholar
  17. Loggini B, Scartazza A, Brugnoli E, Navari-Izzo F (1999) Antioxidative defense system, pigment composition, and photosynthetic efficiency in two wheat cultivars subjected to drought. Plant Physiol 119:1091–1099PubMedCrossRefGoogle Scholar
  18. Marques da Silva J, Arrabaça MC (2004) Photosynthesis in the water-stressed C4 grass Setaria sphacelata is mainly limited by stomata with both rapidly and slowly imposed water deficits. Physiol Plant 121:409–420CrossRefGoogle Scholar
  19. Martinoia E, Schramm MJ, Kaiser G, Kaiser WM, Heber U (1986) Transport of anions in isolated vacuoles. 1. Permeability to anions and evidence for a Cl-uptake system. Plant Physiol 80:895–901PubMedCrossRefGoogle Scholar
  20. McKersie BD, Stinson RH (1980) Effect of dehydration on leakage and membrane structure in Lotus corniculatus L. seeds. Plant Physiol 66:316–320PubMedCrossRefGoogle Scholar
  21. Money NP (1989) Osmotic pressure of aqueous polyethylene glycols. Relationship between molecular weight and vapor pressure deficit. Plant Physiol 91:766–769PubMedCrossRefGoogle Scholar
  22. Ögren E, Öquist G (1985) Effects of drought on photosynthesis, chlorophyll fluorescence and photoinhibition susceptibility in intact willow leaves. Planta 166:380–388CrossRefGoogle Scholar
  23. Palta JP, Levitt J, Stadelmann EJ (1977) Freezing injury in onion bulb cells. I. Evaluation of the conductivity methods and analysis of ion and sugar efflux from injured cells. Plant Physiol 60:393–397PubMedCrossRefGoogle Scholar
  24. Pinheiro HA, Silva JV, Endres L, Ferreira VM, Câmara CA, Cabral FF, Oliveira JF, Carvalho LWT, Santos JM, Santos-Filho BC (2008) Leaf gas exchange, chloroplastic pigments and dry matter accumulation in castor bean (Ricinus communis L.) seedlings subjected to salt stress conditions. Ind Crop Prod 27:385–392CrossRefGoogle Scholar
  25. Prášil I, Zámečník J (1998) The use of a conductivity measurement method for assessing freezing injury. I. Influence of leakage time, segment number, size and shape in a sample on evaluation of the degree of injury. Environ Exp Bot 40:1–10CrossRefGoogle Scholar
  26. Premachandra GS, Saneoka H, Ogata S (1989) Nutrio-physiological evaluation of polyethylene glycol test of cell membrane stability in maize. Crop Sci 29:1287–1292CrossRefGoogle Scholar
  27. Premachandra GS, Ogata S, Saneoka H (1990) Cell membrane stability and leaf water relations as affected by nitrogen nutrition under water stress in maize. Soil Sci Plant Nutr 36:653–659CrossRefGoogle Scholar
  28. Rovere ELL, Avzaradel AC, Monteiro JMG (2009) Potential synergy between adaptation and mitigation strategies: production of vegetables oils and biodiesel in northeastern Brazil. Clim Res 40:233–239CrossRefGoogle Scholar
  29. Saneoka H, Moghaieb REA, Premachandra GS, Fujita K (2004) Nitrogen nutrition and water stress effects on cell membrane stability and leaf water relations in Agrostis palustris Huds. Environ Exp Bot 52:131–138CrossRefGoogle Scholar
  30. Sayar R, Khemira H, Kameli A, Mosbahi M (2008) Physiological tests as peredictive appreciation for drought tolerance in durum wheat (Triticum durum Desf.). Agron Res 6:79–90Google Scholar
  31. Schapendonk AHCM, Spitters CJT, Groot PJ (1989) Effects of water stress on photosynthesis and chlorophyll fluorescence of five potato cultivar. Potato Res 32:17–32CrossRefGoogle Scholar
  32. Senaratna T, McKersie BD (1983) Characterization of solute efflux from dehydration injured soybean (Glycine max L. Merr) seeds. Plant Physiol 72:911–914PubMedCrossRefGoogle Scholar
  33. Thiele A, Krause GH, Winter K (1998) In situ study of photoinhibition of photosynthesis and xanthophyll cycle activity in plants growing in natural gaps of the tropical forest. Aust J Plant Physiol 25:189–195CrossRefGoogle Scholar
  34. Vasquez-Tello A, Zuily-Fodil Y, Phan Thi AT, Vieira da Silva JB (1990) Electrolyte and Pi leakage and soluble sugar content as physiological test for screening resistance to water stress in Phaseolus and Vigna species. J Exp Bot 41:827–832CrossRefGoogle Scholar
  35. Vieira da Silva JB (1976) Water stress, ultrastructure and enzymatic activity. In: Lange OL, Kapen L, Schulze ED (eds) Water and plant life: problems and modern approaches. Springer, Berlin, pp 207–224CrossRefGoogle Scholar
  36. Wang JZ, Cui LJ, Wang Y, Li JL (2009) Growth, lipid peroxidation and photosynthesis in two tall fescue cultivars differing in heat tolerance. Biol Plant 53:237–242CrossRefGoogle Scholar
  37. Zhao L, Liu F, Xu W, Di C, Zhou S, Xue Y, Yu J, Su Z (2009) Increased expression of OsSPX1 enhances cold/subfreezing tolerance in tobacco and Arabidopsis thaliana. Plant Biotech J 7:550–561CrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2012

Authors and Affiliations

  • Ana Paula de Faria
    • 1
  • José Pires Lemos-Filho
    • 1
  • Luzia Valentina Modolo
    • 1
  • Marcel Giovanni Costa França
    • 1
    Email author
  1. 1.Departamento de BotânicaUniversidade Federal de Minas GeraisBelo HorizonteBrazil

Personalised recommendations