Acta Physiologiae Plantarum

, Volume 35, Issue 1, pp 31–40 | Cite as

Capacity to control oxidative stress-induced caspase-like activity determines the level of tolerance to salt stress in two contrasting maize genotypes

  • Marshall Keyster
  • Ashwil Klein
  • Morné Du Plessis
  • Alex Jacobs
  • Abidemi Kappo
  • Gábor Kocsy
  • Gábor Galiba
  • Ndiko Ludidi
Original Paper

Abstract

The response of two maize (Zea mays L.) genotypes, named GR (salt-tolerant) and SK (salt-sensitive), to salt stress (150 mM NaCl) was investigated under controlled environmental growth conditions. Genotype SK experienced more oxidative damage than the GR genotype when subjected to salt stress, which corresponded to higher O2 production rate and H2O2 content in the SK genotype than the GR genotype. Induction of caspase-like activity in response to salt stress was stronger in the SK genotype than in the GR genotype. On the other hand, induction of antioxidant enzyme activity to scavenge O2 and H2O2 in response to salt stress was weaker in the SK genotype than in the GR genotype. Consequently, the higher level of oxidative damage in the SK genotype in response to salt stress was manifested as more extensive cell death and biomass reduction in the SK genotype than it was in the GR genotype. Our results suggest that a direct relationship exists between salt stress-induced oxidative damage and cell death-inducing caspase-like activity, with tolerance to the salt stress being controlled by the efficiency of the plant antioxidant enzymes in limiting salt stress-induced oxidative damage and thus limiting cell death-inducing caspase-like activity.

Keywords

Antioxidant enzymes Caspase-like activity Cell death Salt stress Oxidative stress Lipid peroxidation 

Abbreviations

Ac-DEVD-pNA

N-Acetyl-Asp-Glu-Val-Asp-p-Nitroanilide

ANOVA

Analysis of variance

APX

Ascorbate peroxidase

EDTA

Ethylenediaminetetraacetic acid

DW

Dry weight

GSH

Glutathione

GPX

Glutathione peroxidase

MDA

Malondialdehyde

MES

2-(N-Morpholino)ethanesulfonic acid

NADPH

Nicotinamide adenine dinucleotide phosphate

PMSF

Phenylmethylsulfonyl fluoride

ROS

Reactive oxygen species

SDS

Sodium dodecyl sulphate

SOD

Superoxide dismutase

TCA

Trichloroacetic acid

WST-1

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium

XTT

2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carbox-anilide

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2012

Authors and Affiliations

  • Marshall Keyster
    • 1
    • 2
  • Ashwil Klein
    • 1
  • Morné Du Plessis
    • 2
  • Alex Jacobs
    • 1
  • Abidemi Kappo
    • 2
  • Gábor Kocsy
    • 3
  • Gábor Galiba
    • 3
  • Ndiko Ludidi
    • 1
  1. 1.Department of BiotechnologyUniversity of the Western CapeBellvilleSouth Africa
  2. 2.Institute for Plant BiotechnologyStellenbosch UniversityMatielandSouth Africa
  3. 3.Agricultural Institute, Hungarian Academy of Sciences, Center for Agricultural ResearchMartonvásárHungary

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