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Acta Physiologiae Plantarum

, Volume 34, Issue 5, pp 1679–1688 | Cite as

Photosynthetic activity and leaf antioxidative responses of Atriplex portulacoides subjected to extreme salinity

  • Maali Benzarti
  • Kilani Ben Rejeb
  • Ahmed Debez
  • Dorsaf Messedi
  • Chedly Abdelly
Original Paper

Abstract

Responses of Atriplex portulacoides upon 40-day-long exposure to salinity (0–1,000 mM NaCl) were investigated. Mother plants originated from a sabkha located in a semi-arid region of Tunisia. The plant relative growth rate and leaf expansion increased significantly at 200 mM NaCl but decreased at higher salinities. Interestingly, the plants survived salinity as high as 1,000 mM NaCl without displaying salt-induced toxicity symptoms. Despite significant increase in leaf Na+ and Cl concentrations upon salt treatment, no significant effect on leaf relative water content was registered. Chlorophyll contents and the gas exchange parameters showed a significant stimulation at the optimal salinity (200 mM NaCl) followed by a decline at higher salinities. Extreme salinity hardly impacted the maximal efficiency of photosystem II photochemistry (F v/F m), but a marked decrease in the relative quantum yield of photosystem II (ФPSII) was observed, along with a significant increase in non-photochemical quenching (NPQ). Leaf malondialdehyde and carotenoid contents were generally unaffected following salt exposure, whereas those of anthocyanins, polyphenols, and proline increased significantly, being maximal at 1,000 mM NaCl. Leaf superoxide dismutase (EC 1.15.1.1), ascorbate peroxidase (EC 1.11.1.11), and glutathione reductase (EC 1.6.4.2) activities were significantly stimulated by salinity, whereas catalase (EC 1.11.1.6) activity was maximal in the 0–400 mM NaCl range. As a whole, protecting the photosynthetic machinery from salt-induced photodamage together with the sustained antioxidant activity may account for the performance of A. portulacoides under high salinity.

Keywords

Halophyte Oxidative stress Photosynthesis Pigments Salinity 

Abbreviations

A

Net photosynthetic rate

APX

Ascorbate peroxidase

AsA

Ascorbate

CAT

Catalase

Chl

Chlorophyll

Ci

Intracellular CO2 concentration

E

Transpiration rate

F0

Minimal fluorescence

Fm

Maximal fluorescence

Fv/Fm

Maximum quantum efficiency of PSII photochemistry

gs

Stomatal conductance

GR

Glutathione reductase

GSH

Glutathione

LWR

Leaf weight rate

MDA

Malonyldialdehyde

NPQ

Non-photochemical quenching

NADP+

Nicotinamide dinucleotide phosphate

1O2

Singlet oxygen

O2˙

Anion superoxide

OH˙

Hydroxyl radicals

PSII

Photosystem II

RGR

Relative growth rate

ROS

Reactive oxygen species

SLR

Specific leaf rate

SOD

Superoxide dismutase

ФPSII

Relative quantum yield of PSII

Notes

Acknowledgments

This work was supported by the Tunisian Ministry of Higher Education and Scientific Research (LR10CBBC02) and the Tunisian-French CMCU (Comité Mixte de Coopération Universitaire) network (08G0917).

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2012

Authors and Affiliations

  • Maali Benzarti
    • 1
  • Kilani Ben Rejeb
    • 1
    • 2
  • Ahmed Debez
    • 1
  • Dorsaf Messedi
    • 1
  • Chedly Abdelly
    • 1
  1. 1.Laboratoire des Plantes ExtrêmophilesCentre de Biotechnologie de Borj-Cedria (CBBC)Hammam-LifTunisia
  2. 2.Physiologie Cellulaire et Moléculaire des Plantes, UR5EAC 7180 CNRS, Université Pierre et Marie Curie (UPMC)Paris Cedex 05France

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