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Acta Physiologiae Plantarum

, Volume 34, Issue 4, pp 1345–1351 | Cite as

Effects of sucrose concentration and exogenous hormones on growth and periplocin accumulation in adventitious roots of Periploca sepium Bunge

  • Jian Zhang
  • Wen-Yuan GaoEmail author
  • Juan Wang
  • Xing-lin Li
Original Paper

Abstract

Periploca sepium adventitious roots were cultured on 0.5 Murashige and Skoog solid media supplemented with exogenous hormones of different types and various concentrations, and with sucrose of different concentrations. Auxins (indole butyric acid (IBA) and naphthalene acetic acid (NAA)) and cytokinins (6-benzylaminopurine (BA) and kinetin (KT)) were selected as exogenous hormones for adventitious root proliferation. Compared with other hormones, IBA was the suitable auxin for adventitious root proliferation. Under this circumstance, every root explant generates 10–15 adventitious roots (1- to 2-cm long) after 30 days. However, nothing but callus was induced on the root explants when NAA was added into the medium and the same result was achieved when auxins (IBA or NAA) were added into the media together with cytokinins (BA or KT). The suitable concentration of IBA for adventitious root proliferation was 1–2 mg/l, when every root explant generated 10–20 adventitious roots (1- to 2-cm long). The optimum concentration of IBA for periplocin accumulation was 1 mg/l, when the periplocin content reached 95.46 μg/g. With regard to the investigation of sucrose concentration, 2–3% (w/v) sucrose was favorable for adventitious root proliferation as every root explant in this concentration generated 10–20 adventitious roots (1- to 2-cm long). The highest periplocin content (101.56 μg/g) was achieved at 5% (w/v) sucrose, whereas the periplocin content at 5% (w/v) sucrose did not show significant difference from the periplocin content (95.38 and 98.47 μg/g, respectively) at 3% (w/v) or 4% (w/v) sucrose.

Keywords

Periploca sepium Bunge Adventitious root proliferation Exogenous hormones Sucrose concentration Periplocin 

Abbreviations

MS

Murashige and Skoog

IBA

Indole butyric acid

NAA

Naphthalene acetic acid

6-BA

6-Benzylaminopurine

KT

Kinetin

LDH

Lactate dehydrogenase

Notes

Acknowledgments

This research was financially supported by the Main Project of Tianjin Science and Technology Support Program (09ZCKFSH01100).

References

  1. Baque MA, Hahn EJ, Paek KY (2010a) Growth, secondary metabolite production and antioxidant enzyme response of Morinda citrifolia adventitious root as affected by auxin and cytokinin. Plant Biotechnol Rep 4(2):109–116CrossRefGoogle Scholar
  2. Baque MA, Hahn EJ, Paek KY (2010b) Induction mechanism of adventitious root from leaf explants of Morinda citrifolia as affected by auxin and light quality. In Vitro Cell Dev Biol Plant 46(1):71–80CrossRefGoogle Scholar
  3. Baque MA, Elgirban A, Lee EJ, Paek KY (2011) Sucrose regulated enhanced induction of anthraquinone, phenolics, flavonoids biosynthesis and activities of antioxidant enzymes in adventitious root suspension cultures of Morinda citrifolia (L.) Acta Physiol Plant. doi: 10.1007/s11738-0837-2
  4. Bollmark M, Eliasson L (1986) Effects of exogenous cytokinins on root formation in pea cuttings. Physiol Plant 68:662–666CrossRefGoogle Scholar
  5. Calamar A, Klerk GJ (2002) Effect of sucrose on adventitious root regeneration in apple. Plant Cell Tiss Org Cult 70:207–212CrossRefGoogle Scholar
  6. Chen YP, Li GX, Wu P et al (2005) The reason and precaution of clinically adverse reaction of coxtex periplocae radicis. Chin Hosp Pharm J 25(7):689–690Google Scholar
  7. Cui XH, Charkrabarty D, Lee EJ, Paek KY (2010a) Production of adventitious roots and secondary metabolites by Hypericum perforatum L. in a bioreactor. Bioresour Technol 101(12):4708–4716PubMedCrossRefGoogle Scholar
  8. Cui XH, Murthy HN, Wu CH, Paek KY (2010b) Sucrose-induced osmotic stress affects biomass, metabolite, and antioxidant levels in root suspension cultures of Hypericum perforatum L. Plant Cell Tiss Organ Cult 103:7–14CrossRefGoogle Scholar
  9. Dornenburg H, Knorr D (1995) Strategies for the improvement of secondary metabolite production in plant cell cultures. Enzyme Microb Technol 17(8):674–684CrossRefGoogle Scholar
  10. Endress R (1994) Plant cells as producers of secondary compounds. In: Endress R (ed) Plant cell biotechnology. Springer, Berlin, pp 121–242Google Scholar
  11. Fitter AH (1996) Characteristics and functions of root systems. In: Waisel Y, Eshel A, Kafkafi U (eds) Plant roots: the hidden half. Marcel Dekker, Inc., New York, pp 1–20Google Scholar
  12. Haissig BE (1982) Carbohydrate and amino acid concentrations during adventitious root primordia development in Pinus banksiana Lamb. Cuttings. For Sci 28:813–821Google Scholar
  13. Humphries EC (1960) Inhibition of root development on petioles and hypocotyls of dwarf bean (Phaseolus vulgaris) by kinetin. Physiol Plant 13:659–663CrossRefGoogle Scholar
  14. Kim YS, Hahn EJ, Yeung EC, Paek KY (2003) Lateral root development and saponin accumulation as affected by IBA or NAA in adventitious root cultures of Panax ginseng CA Meyer. In Vitro Cell Dev Biol Plant 39:245–249CrossRefGoogle Scholar
  15. Kit SM (1964) Comparative biological effects of strophanthin-k, corelborin-p, cymarin and periplocin on the cat heart in lesions induced with zinc hydroxide. Farmakol Toksikol 27:699–701PubMedGoogle Scholar
  16. Kollarova K, Liskova D, Kakoniova D, Lux A (2004) Effect of auxins on Karwinskia humboldtiana root cultures. Plant Cell Tiss Org Cult 79:213–221CrossRefGoogle Scholar
  17. Liu JH, Reid DM (1992) Adventitious rooting in hypocotyls of sunflower (Helianthus annuus) seeding. IV. The role of changes in endogenous free and conjugated indole-3-acetic acid. Physiol Plant 86(2):285–292CrossRefGoogle Scholar
  18. Medford JI, Horgan R, EI-Sawi Z, Klee HJ (1989) Alterations of endogenous cytokinins in transgenic plants using a chimeric isopentenyl transferase gene. Plant Cell 1(4):403–413PubMedGoogle Scholar
  19. Pagnussat GC, Lanteri ML, Lombardo MC, Lamattina L (2004) Nitric oxide mediates the indole acetic acid induction activation of a mitogen-activated protein kinase cascade involved in adventitious root development. Plant Physiol 135:279–285PubMedCrossRefGoogle Scholar
  20. Ren XL, Liu H, Qi AD et al (2007) Improving of simultaneous determination of periplocin and 4-methoxy salicylic aldehyde in cortex periplocae with HPLC method. Tianjin J Tradit Chin Med 24(3):252–254Google Scholar
  21. Skoog F, Miller CO (1957) Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp Soc Exp Biol 54(11):118–130Google Scholar
  22. Taiz L, Zeiger E (1991) Ethylene and abscisic acid. In: Taiz L, Zeiger E (eds) Plant Physiology. The Benjamin/Cummings Publishing Company, Redwood City, pp 473–489Google Scholar
  23. Tian HQ, Russell SD (1998) Culture-induced changes in osmolality of tobacco cell suspensions using four exogenous sugars. Plant Cell Tiss Org Cult 55:9–13CrossRefGoogle Scholar
  24. Umehara K, Sumii N, Satoh H, Miyase T, Kuroyanagi M, Ueno A (1995) Studies on differentiation inducers. V. Steroid glycosides from periplocae radicis cortex. Chem Pharm Bull (Tokyo) 43:1565–1568CrossRefGoogle Scholar
  25. Wang TX, Li JY, Hu ZH (2003) Morphogenesis and structural development of the root tuber of Rehmannia glutinosa cv Hueichingensis. Acta Bot Boreal Occident Sin 23(7):1217–1223Google Scholar
  26. Wang H, Zu Y, Liu H (2007) Efficient rooting and root development after transfer of regenerated plantlets of Camptotheca acuminate. Eurasian J. For Res 10(2):179–184Google Scholar
  27. Wang Q, Ren XL, Wang Y et al (2008) Study on excretion of periplocin in rats. J Tianjin Univ Tradit Chin Med 27(1):29–32Google Scholar
  28. Wang XY, Gao XM, Liu H, Liu Y, Jiang M, Hu LM, Zhang BL (2010) Gene expression profiling of the proliferative effect of periplocin on mouse cardiac microvascular endothelial cells. Chin J Integr Med 16(1):33–40PubMedCrossRefGoogle Scholar
  29. Wu CH, Dewir YH, Hahn EJ, Paek KY (2006) Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots of Echinacea angustifolia. J Plant Biol 49:193–199CrossRefGoogle Scholar
  30. Xu LQ, Lu HZ, Zhang YL (1998) Clinical observation on treatment of 147 cases of chronic congestive heart—failure by Bei Wujiapi mixture. Yunnan J Tradit Chin Med Mater Med 19(4):29–31Google Scholar
  31. Zhang J, Gao WY, Li XL, Wang J, Li LM (2010) Induction of Periploca sepium in vitro plantlet and studies on dynamic accumulation of periplocin. Chin J Chin Mater Med 35(18):2392–2394Google Scholar
  32. Zhang J, Gao WY, Wang J, Li XL (2011) Effects of explant types and media salt strength on growth and secondary metabolite accumulation in adventitious roots of Periploca sepium Bunge. Acta Physiol Plant 33:2447–2452CrossRefGoogle Scholar
  33. Zimmermann MH, Ziegler H (1975) List of sugars and sugar alcohols in sieve-tube exudates. In: Zimmermann MH, Milburn JA (eds) Encyclopedia of plant physiology. New series, vol 1. Springer, Berlin, pp 480–503Google Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2012

Authors and Affiliations

  • Jian Zhang
    • 1
  • Wen-Yuan Gao
    • 2
    Email author
  • Juan Wang
    • 2
  • Xing-lin Li
    • 3
  1. 1.School of Traditional Chinese Materia MedicaTianjin University of Traditional Chinese MedicineTianjinPeople’s Republic of China
  2. 2.School of Pharmaceutical Science and TechnologyTianjin UniversityTianjinPeople’s Repubic of China
  3. 3.Institute of Biological Engineering of Traditional Chinese MedicineTianjin University of Science and TechnologyTianjinPeople’s Republic of China

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