Acta Physiologiae Plantarum

, Volume 34, Issue 2, pp 617–629

Antioxidant system in programmed cell death of sycamore (Acer pseudoplatanus L.) cultured cells

  • Nicla Contran
  • Mariagrazia Tonelli
  • Paolo Crosti
  • Raffaella Cerana
  • Massimo Malerba
Original Paper

Abstract

Reactive oxygen species (ROS) have pleiotropic effects in plants. ROS can lead to cellular damage and death or play key roles in control and regulation of biological processes, such as programmed cell death (PCD). This dual role of ROS, as toxic or signalling molecules, is possible because plant antioxidant system (AS) is able to achieve a tight control over ROS cellular levels, balancing properly their production and scavenging. AS response in plant PCD has been clearly described only in the hypersensitive response in incompatible plant–pathogen interactions and in the senescence process and has not been completely unravelled. In sycamore (Acer pseudoplatanus L.) cultured cells PCD can be induced by Fusicoccin (Fc), Tunicamycin (Tu), and Brefeldin A (Ba). These chemicals induce comparable PCD time course and extent, while H2O2 production is detectable only in Fc- and, to a lesser extent, in Ba-treated cells. In this paper the AS has been investigated during PCD of sycamore cells, measuring the effects of the three inducers on the cellular levels of non-enzymatic and enzymatic antioxidants. Results show that the AS behaviour is different in the PCD induced by the three chemicals. In Fc-treated cells AS is mainly devoted to decrease the concentration of toxic intracellular H2O2 levels. On the contrary, in cells treated with Tu and Ba, the cell redox state is shifted to a more reduced state and the enzymatic AS is partially down-regulated, allowing ROS to act as signalling molecules.

Keywords

Reactive oxygen species Programmed cell death Antioxidant enzyme Ascorbate–glutathione cycle Acer pseudoplatanus L. 

Abbreviations

APX

Ascorbate peroxidase

AS

Antioxidant system

ASA

Ascorbic acid

Ba

Brefeldin A

CAT

Catalase

DHA

Dehydroascorbic acid

DR

Dehydroascorbate reductase

Fc

Fusicoccin

GPX

Glutathione peroxidase

GR

Glutathione reductase

GSH

Reduced glutathione

GSSG

Oxidized glutathione

H2O2

Hydrogen peroxide

MDA

Malondialdehyde

MR

Monodehydroascorbate reductase

PCD

Programmed cell death

ROS

Reactive oxygen species

SOD

Superoxide dismutase

Tu

Tunicamycin

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2011

Authors and Affiliations

  • Nicla Contran
    • 1
  • Mariagrazia Tonelli
    • 1
  • Paolo Crosti
    • 1
  • Raffaella Cerana
    • 2
  • Massimo Malerba
    • 1
  1. 1.Department of Biotechnology and BiosciencesUniversity of Milano-BicoccaMilanItaly
  2. 2.Department of Environmental SciencesUniversity of Milano-BicoccaMilanItaly

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