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Acta Physiologiae Plantarum

, Volume 34, Issue 2, pp 533–540 | Cite as

Changes in the transcript levels of microtubule-associated protein MAP65-1 during reorientation of cortical microtubules in azuki bean epicotyls

  • Kouichi SogaEmail author
  • Toshihisa Kotake
  • Kazuyuki Wakabayashi
  • Takayuki Hoson
Original Paper

Abstract

The relation between orientation of cortical microtubules and the transcript levels of a 65 kDa microtubule-associated protein (VaMAP65-1) was investigated along epicotyls of azuki bean (Vigna angularis) seedlings. In addition, the effects of 1-aminocyclopropane-1-carboxylic acid (ACC), the immediate precursor of ethylene, and hypergravity, a gravitational force exceeding 1g, on the relation were examined. The percentage of cells with longitudinal microtubule increased, whereas that with transverse microtubules decreased from the apical to the basal regions of epicotyls. The transcript levels of VaMAP65-1 decreased toward the basal region. ACC induced reorientation of cortical microtubules from transverse to longitudinal directions and down-regulation of VaMAP65-1 expression. Hypergravity also induced reorientation of cortical microtubules and down-regulation of the expression. Strong correlations were observed between the percentage of cells with longitudinal or transverse microtubules and the transcript levels of VaMAP65-1. These results suggest that down-regulation of VaMAP65-1 expression is at least partly involved in the regulation of the orientation of cortical microtubules in azuki bean epicotyls.

Keywords

Azuki bean (Vigna angularisCortical microtubule orientation Epicotyl region Ethylene Hypergravity 65 kDa Microtubule-associated protein (MAP65) 

Abbreviations

ACC

1-Aminocyclopropane-1-carboxylic acid

PBS

Phosphate-buffered saline

RT-PCR

Reverse transcription-PCR

VaMAP65-1

Vigna angularis microtubule-associated protein 65-1

Notes

Acknowledgments

The present study was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, a Grant for Ground-based Research for Space Utilization from Japan Space Forum, and by Sasakawa Scientific Research Grant from the Japan Science Society.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2011

Authors and Affiliations

  • Kouichi Soga
    • 1
    Email author
  • Toshihisa Kotake
    • 2
  • Kazuyuki Wakabayashi
    • 1
  • Takayuki Hoson
    • 1
  1. 1.Department of Biology and GeosciencesGraduate School of Science, Osaka City UniversityOsakaJapan
  2. 2.Division of Life ScienceGraduate School of Science and Engineering, Saitama UniversitySaitamaJapan

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